Dual NMR for probing intrinsic bone structure and a potential gut‐bone axis in ovariectomized rats

2021 ◽  
Author(s):  
Weiwei He ◽  
Louise M. A. Jakobsen ◽  
Line F. Zachariassen ◽  
Axel K. Hansen ◽  
Henrik J. Andersen ◽  
...  
Keyword(s):  
Bone Structure ◽  
Ovariectomized Rats

scholarly journals Zhuang-Gu-Fang Treats Osteoporosis in Ovariectomized Rats by Increasing the Osteogenesis-Related Factors Leptin, Ghrelin, and PYY

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Yuanjin Chen ◽  
Rui Bai ◽  
Wenhui Chen ◽  
Shuanglei Li ◽  
Yunxia Jiang
Keyword(s):  
Bone Metabolism ◽  
Therapeutic Effect ◽  
Wistar Rats ◽  
Dose Group ◽  
Bone Structure ◽  
Ovariectomized Rats ◽  
Type I ◽  
Mineral Density ◽  
Related Factors ◽  
Expression Levels

Zhuang-Gu-Fang is a Chinese medicinal compound mixture, which is mainly composed of traditional remedies like the Epimedium Herb, Astragalus, and Eucommia among many others. The study is aimed at investigating the therapeutic effect of Zhuang-Gu-Fang in ovariectomized rats. Fifty six-month-old Wistar rats were randomly selected and divided into 5 groups (n = 10), namely, model group, positive group, low-dose Chinese medicine group, medium-dose group, and high-dose group. Another 10 sham operation Wistar rats were taken as a negative control group. After 3 months of intervention, the bone mineral density (BMD), procollagen type I N-peptide (PINP), beta C-terminal cross-linked telopeptides of type I collagen carboxyl-terminal peptide (β-CTX), Leptin, Ghrelin, and Peptide YY (PYY) of each group were measured. Besides, the ultrastructure of bone structure and osteoblasts was also observed by transmission electron microscopy. Western blot method was used to detect the expression levels of Leptin and Ghrelin in bone tissue, and RT-PCR detected the mRNA expression levels of Leptin and Ghrelin. BMD test indicated that Zhuang-Gu-Fang could effectively prevent the loss of tibia bone in ovariectomized rats. Histomorphology analysis showed that Zhuang-Gu-Fang could preserve trabecular bone structure integrity and improve osteoblast ultrastructure. Notably, the study found out that Zhuang-Gu-Fang worked through balancing the bone metabolism via increasing bone formation/resorption ratio. Additionally, Zhuang-Gu-Fang highlighted the recovery effects in multiple levels of osteogenesis- and osteanagenesis-related factors Leptin, Ghrelin, and PYY. Conclusively, the study proved the therapeutic potential of the Zhuang-Gu-Fang for postmenopausal osteoporosis (PMOP) and further revealed that its therapeutic effect was related to the balance of bone metabolism and the recovery effects of bone-related factors Leptin, Ghrelin, and PYY.

scholarly journals Regional Acceleratory Phenomenon after Orthodontic Force Exertion in Ovariectomized Rats

2018 ◽  
Vol 69 (2) ◽  
pp. 925
Author(s):  
A. I. TSOLAKIS ◽  
L. KHALDI ◽  
I. BITSANIS ◽  
I. A. DONTAS
Keyword(s):  
Bone Structure ◽  
Mature Female ◽  
Ovariectomized Rats ◽  
Osteoporotic Bone ◽  
Limited Information ◽  
Orthodontic Force ◽  
Ovx Rats ◽  
Female Wistar Rats ◽  
Regional Acceleratory Phenomenon ◽  
Orthodontic Forces

The application of orthodontic forces may be one of the factors that produce a regional acceleratory phenomenon (RAP) in mandibular and maxillary bones. The effect of exerted forces on bone tissue ahead of their point of application has not been extensively studied. Moreover, limited information exists regarding this phenomenon on osteoporotic bone. The study aim was to examine the role of orthodontic forces on the expression of RAP in normal and osteoporotic mature rats. Thirty-six eight-month-old skeletally mature female Wistar rats, half of which had been previously ovariectomized (OVX) at the age of 6 months, were subjected to orthodontic movement of the upper right first molar. An orthodontic force of 60 gr* was generated through a closed coil spring for 14 days. The maxillae were then removed and the area ahead of the first molar was examined histologically. On the side of orthodontic force application, distortion of bone structure and woven bone formation were observed in non-OVX rats, whereas in the OVX rats, extensive remodeling was apparent. In conclusion, the application of orthodontic forces on both normal and osteoporotic mature rats in the present study created a RAP ahead of the loaded teeth demonstrated histologically, indicating increased bone resorption and formation in the OVX rats.

scholarly journals Direct administration of zoledronate acid improves bone structure in local osteoporotic lesion of ovariectomized rats

2013 ◽  
Author(s):  
Yohei Matsuo ◽  
Masafumi Kashii ◽  
Tsuyoshi Sugiura ◽  
Tokimitsu Morimoto ◽  
Hirotsugu Honda ◽  
...  
Keyword(s):  
Bone Structure ◽  
Ovariectomized Rats ◽  
Direct Administration

scholarly journals 7. Density of Lumbal Vertebrae Bone Ovariectomized Rat (Rattus Norvegicus) Given the Extract Sipatah – patah ( Cissus quadrangularis Salisb )

2017 ◽  
Vol 11 (1) ◽  
pp. 39-44
Author(s):  
Putri Dewi ◽  
Mustafa Sabri ◽  
Erdiansyah Rahmi ◽  
M Jalaluddin ◽  
Nuzul Asmilia ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Bone Repair ◽  
Bone Structure ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Density Level ◽  
Osteoblast Activity ◽  
Cissus Quadrangularis ◽  
Stem Extract ◽  
High Level

The aims of this research was to determine the density level of lumbal vertebrae on ovariectomized rats administered with Cissus qudrangula Salisb stem extract with various doses. In this research twelve female rats were randomizedly divided into 4 groups with 3 treatment repetition. K1-groups were ovariectomized rats without Cissus quadrangula Salisb trunct extract administration; K2, K3, and K4, were ovariectomized and given with Cissus quadrangula Salisb extract  with 500 mg/kg/Bw, 700 mg/kg/Bw, and 900 mg/kg/Bw as doses perday for 30 days. On day-31, rats were euthanized using chloroform and the os lumbal-5 were collected, followed with histological preparations. Research parameters were the density of active osteoblasts, passive osteoblasts, and osteoclasts. The results showed that the damage in K1 was clearly marked with the high number of osteoclasts, K2 and K3 indicate lower osteoclasts levels than K1 and had repaired bone structure characterized with active cuboidal shaped  osteoblast  activity and passive flat shaped osteoblasts and K4 showed increasing  level of active osteoblasts. The administration of Cissus quadrangula Salisb stem extract at 900 mg/kg/bw for 30 days showed bone repair due to ovariectomy indicated with high level of active osteoblasts.

scholarly journals Swim-trained rats have greater bone mass, density, strength, and dynamics

2001 ◽  
Vol 91 (4) ◽  
pp. 1663-1668 ◽  
Author(s):  
K. J. Hart ◽  
J. M. Shaw ◽  
E. Vajda ◽  
M. Hegsted ◽  
S. C. Miller
Keyword(s):  
Bone Mineral Density ◽  
Bone Mineral ◽  
Bone Structure ◽  
Mass Density ◽  
Weight Bearing ◽  
Femoral Shaft ◽  
Ovariectomized Rats ◽  
Bone Mass Density ◽  
Control Group ◽  
Mineral Density

Weight-bearing exercise is traditionally recommended for improving bone health in postmenopausal women. Effects of swim exercise were studied as an alternative to weight-bearing exercise in ovariectomized rats. Rats in a swim group (Sw, n = 8) swam for 12 wk, 5 days/wk for 60 min per session. A control group (Con, n = 9) engaged in no structured exercise. Femurs were analyzed for bone mineral density and for bone mineral content by dual energy X-ray absorptiometry, biomechanical properties by three-point bending (Instron), and bone structure and formation by histomorphometry. Food intake did not differ among groups. Final body weights were significantly lower in Sw compared with Con ( P< 0.05). Swimmers had significantly greater femoral shaft bone mineral density and content ( P < 0.05) compared with Con. Femurs of the Sw group had greater mechanical properties ( P< 0.05) compared with Con. Histomorphometric data were significantly better in the Sw group compared with Con after the 12-wk intervention ( P < 0.05). In conclusion, data from this study demonstrate some beneficial effects of swim exercise on bone structure, turnover, and strength.

scholarly journals The Effect of Additive Formula Diet on Bone Structure of the Femur in Ovariectomized Rats

2017 ◽  
Vol 26 (2) ◽  
pp. 215-222
Author(s):  
Kanami Sato ◽  
Takahiro Takahashi ◽  
Hiroshi Nakada ◽  
Toshiro Sakae ◽  
Takehiro Watanabe ◽  
...  
Keyword(s):  
Bone Structure ◽  
Ovariectomized Rats ◽  
Formula Diet

scholarly journals Effect of oligofructose or dietary calcium on repeated calcium and phosphorus balances, bone mineralization and trabecular structure in ovariectomized rats

2002 ◽  
Vol 88 (4) ◽  
pp. 365-377 ◽  
Author(s):  
Katharina E. Scholz-Ahrens ◽  
Yahya Açil ◽  
Jürgen Schrezenmeir
Keyword(s):  
Bone Structure ◽  
Bone Mineralization ◽  
Lumbar Vertebra ◽  
Ovariectomized Rats ◽  
Bone Area ◽  
Trabecular Thickness ◽  
Trabecular Bone Area ◽  
Mineral Levels ◽  
Trabecular Number ◽  
Recommended Level

We investigated the effects of dietary oligofructose and Ca on bone structure in ovariectomized rats, using microradiography and histomorphometry. Ninety-six animals were allocated to seven experimental groups: G1, sham-operated; G2–G7, ovariectomized. Semi-purified diets containing 5 g Ca/kg (recommended content) without oligofructose (G1, G2) or with 25, 50 or 100 g oligofructose/kg (G3, G4, G5) or 10 g Ca/kg (high content) without oligofructose (G6) or with 50 g oligofructose/kg (G7) were fed for 16 weeks. At the recommended level of Ca, high oligofructose (G5) increased femur mineral levels in ovariectomized rats, while medium oligofructose did so at high Ca. Increasing Ca in the absence of oligofructose did not increase femur mineral content. Trabecular bone area (%) analysed in the tibia was 10·3 (SEM 1·2) (G1), 7·7 (SEM 0·6) (G2), 9·3 (SEM 0·7) (G3), 9·4 (SEM 1·0) (G4), 9·5 (SEM 0·7) (G5), 10·2 (SEM 0·8) (G6), and 12·6 (SEM 0·8) (G7). At the recommended level of Ca, 25 g oligofructose/kg prevented loss of trabecular area due to increased trabecular thickness, while 50 or 100 g oligofructose/kg increased trabecular perimeter. At high Ca, oligofructose prevented loss of bone area due to increased trabecular number but similar thickness (G7v. G6). When Ca was raised in the presence of oligofructose (G7), trabecular area and cortical thickness were highest, while loss of trabecular connectivity was lowest of all groups. At the same time, lumbar vertebra Ca was higher; 44·0 (SEM 0·8) (G7) compared with 41·6 (SEM 0·8) (G2), 41·4 (SEM 0·7) (G4), and 40·5 (SEM 1·0) mg (G6). We conclude that ovariectomy-induced loss of bone structure in the tibia was prevented but with different trabecular architecture, depending on whether dietary Ca was increased, oligofructose was incorporated, or both. Oligofructose was most effective when dietary Ca was high.

scholarly journals Quality of bone repair in ovariectomized rats with bone lesions treated with phytotherapic and homeopathic Arnica montana

2017 ◽  
Vol 20 (1) ◽  
pp. 25 ◽  
Author(s):  
Edmundo Silva ◽  
Vanessa Fernandes Pereira ◽  
Luana Marotta Reis Vasconcellos ◽  
Felipe Eduardo Oliveira ◽  
Adriana Aigotti Haberbeck Brandão
Keyword(s):  
Bone Repair ◽  
Bone Structure ◽  
The Elderly ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Bone Lesions ◽  
Arnica Montana ◽  
Sham Surgery ◽  
International Data

<p><strong>Objective: </strong>National and international data show that in the next years the elder population tends to have an exponential increase becoming to be significantly higher than other ages.  Among the frequent diseases in the elderly, osteoporosis is a disease that decreases bone mass and deteriorates bone structure causing fragility and a high risk of fracture. This study aimed to evaluate the effect of phytotherapic and homeopathic <em>Arnica montana</em> on bone repair quality and its possible use in the treatment of bone fracture in patients with osteoporosis. <strong>Material and Methods:</strong> This experimental study was performed in Wistar adult female rats divided in 4 groups according to the following treatments: ovariectomized treated with homeopathic<em> A. montana</em> 6CH (OVZ 6CH); ovariectomized treated with phytotherapic <em>A. montana</em> (OVZ TM); ovariectomized with placebo (OVZ PL) and rats with sham surgery and placebo (Sham PL). In a period of 45 days after ovariectomy or sham surgery, all animals got a monocortical lesion. They were euthanized after 6, 12, 18 and 28 days and had the tibiae removed to evaluate dimensions and bone repair by radiographic density, biomechanical test and scanning electronic microscopy (SEM). Data were analyzed by ANOVA and TUKEY tests (p &lt; 0.05). <strong>Results:</strong> Results showed that treatment with <em>Arnica</em> 6CH was better than <em>Arnica</em> TM considering bone  resistance and bone repair quality. <strong>Conclusion: </strong>This study concluded that treatment with homeopathic <em>A. montana</em> was more efficient than with phytotherapic Arnica in bone regeneration in rats with osteoporosis.</p><p><strong>Keywords</strong></p><p><em>Arnica montana</em>; Homeopathy; Osteoporosis; Phytotherapy; Radiographic bone density. </p>

scholarly journals Virgin Coconut Oil Supplementation Prevents Bone Loss in Osteoporosis Rat Model

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Zil Hayatullina ◽  
Norliza Muhammad ◽  
Norazlina Mohamed ◽  
Ima-Nirwana Soelaiman
Keyword(s):  
Bone Loss ◽  
Rat Model ◽  
Bone Structure ◽  
Bone Microarchitecture ◽  
Coconut Oil ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Antioxidant Compounds ◽  
Virgin Coconut Oil ◽  
The Right

Oxidative stress and free radicals have been implicated in the pathogenesis of osteoporosis. Therefore, antioxidant compounds have the potential to be used in the prevention and treatment of the disease. In this study, we investigated the effects of virgin coconut oil (VCO) on bone microarchitecture in a postmenopausal osteoporosis rat model. VCO is a different form of coconut oil as it is rich with antioxidants. Three-month-old female rats were randomly grouped into baseline, sham-operated, ovariectomized control (Ovx), and ovariectomized rats fed with 8% VCO in their diet for six weeks (Ovx+VCO). Bone histomorphometry of the right femora was carried out at the end of the study. Rats supplemented with VCO had a significantly greater bone volume and trabecular number while trabecular separation was lower than the Ovx group. In conclusion, VCO was effective in maintaining bone structure and preventing bone loss in estrogen-deficient rat model.

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