In situ solid-state NMR characterization of pharmaceutical materials: An example of drug-polymer thermal mixing

2020 ◽  
Author(s):  
Jinglin Yin ◽  
Chengbin Huang ◽  
Hanxi Guan ◽  
Zhenfeng Pang ◽  
Yongchao Su ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Thermal Mixing ◽  
Nmr Characterization ◽  
Pharmaceutical Materials

scholarly journals Unambiguous Ex Situ and in Cell 2D 13C Solid-State NMR Characterization of Starch and Its Constituents

2018 ◽  
Vol 19 (12) ◽  
pp. 3817 ◽  
Author(s):  
Alexandre Poulhazan ◽  
Alexandre Arnold ◽  
Dror Warschawski ◽  
Isabelle Marcotte
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Amylose Content ◽  
Amorphous State ◽  
Ex Situ ◽  
Starch Structure ◽  
Nmr Characterization ◽  
Crystalline Domains

Starch is the most abundant energy storage molecule in plants and is an essential part of the human diet. This glucose polymer is composed of amorphous and crystalline domains in different forms (A and B types) with specific physicochemical properties that determine its bioavailability for an organism, as well as its value in the food industry. Using two-dimensional (2D) high resolution solid-state nuclear magnetic resonance (SS-NMR) on 13C-labelled starches that were obtained from Chlamydomonas reinhardtii microalgae, we established a complete and unambiguous assignment for starch and its constituents (amylopectin and amylose) in the two crystalline forms and in the amorphous state. We also assigned so far unreported non-reducing end groups and assessed starch chain length, crystallinity and amylose content. Starch was then characterized in situ, i.e., by 13C solid-state NMR of intact microalgal cells. Our in-cell methodology also enabled the identification of the effect of nitrogen starvation on starch metabolism. This work shows how solid-state NMR can enable the identification of starch structure, chemical modifications and biosynthesis in situ in intact microorganisms, eliminating time consuming and potentially altering purification steps.

Sign in / Sign up

Export Citation Format

Share Document