scholarly journals Anisotropic diffusion in stretched hydrogels containing erythrocytes: evidence of cell-shape distortion recorded by PGSE NMR spectroscopy

2016 ◽  
Vol 55 (5) ◽  
pp. 438-446 ◽  
Author(s):  
Dmitry Shishmarev ◽  
Konstantin I. Momot ◽  
Philip W. Kuchel
Keyword(s):  
Nmr Spectroscopy ◽  
Anisotropic Diffusion ◽  
Cell Shape ◽  
Shape Distortion ◽  
Pgse Nmr

scholarly journals Relationship between Li+ diffusion and ion conduction for single-crystal and powder garnet-type electrolytes studied by 7Li PGSE NMR spectroscopy

2019 ◽  
Vol 21 (42) ◽  
pp. 23589-23597 ◽  
Author(s):  
Kikuko Hayamizu ◽  
Yasuhiko Terada ◽  
Kunimitsu Kataoka ◽  
Junji Akimoto ◽  
Tomoyuki Haishi
Keyword(s):  
Nmr Spectroscopy ◽  
Ionic Conductivity ◽  
Single Crystal ◽  
Ion Conduction ◽  
Diffusion Constants ◽  
Pgse Nmr

Li-diffusion constants of single-crystal and powder garnets were determined and plotted versus ionic conductivity. Estimated NE carrier numbers were larger than atomic Li numbers for metal containing garnets and insensitive to temperature.

Thermal dynamics of lithium salt mixtures of ionic liquid in water by PGSE NMR spectroscopy

RSC Advances ◽  
2016 ◽  
Vol 6 (43) ◽  
pp. 36394-36406 ◽  
Author(s):  
Raju Nanda
Keyword(s):  
Ionic Liquid ◽  
Nmr Spectroscopy ◽  
Lithium Salt ◽  
Thermal Dynamics ◽  
Salt Mixtures ◽  
Pgse Nmr

The dynamics of ionic liquid–H2O–Li+ salt mixtures have been studied through PGSE NMR spectroscopy.

Modulated polarization microscopy displays the dynamics of cytoskeletal structures in living, motile cells

1995 ◽  
Vol 53 ◽  
pp. 902-903
Author(s):  
J. R. Kuhn ◽  
M. Poenie
Keyword(s):  
Mitotic Spindle ◽  
Actin Filaments ◽  
Cell Shape ◽  
Dynamic Structure ◽  
Living Cells ◽  
Cytoskeletal Proteins ◽  
Polarization Microscopy ◽  
Video Enhancement ◽  
Ultrastructural Studies ◽  
Motile Cells

Cell shape and movement are controlled by elements of the cytoskeleton including actin filaments an microtubules. Unfortunately, it is difficult to visualize the cytoskeleton in living cells and hence follow it dynamics. Immunofluorescence and ultrastructural studies of fixed cells while providing clear images of the cytoskeleton, give only a static picture of this dynamic structure. Microinjection of fluorescently Is beled cytoskeletal proteins has proved useful as a way to follow some cytoskeletal events, but long terry studies are generally limited by the bleaching of fluorophores and presence of unassembled monomers.Polarization microscopy has the potential for visualizing the cytoskeleton. Although at present, it ha mainly been used for visualizing the mitotic spindle. Polarization microscopy is attractive in that it pro vides a way to selectively image structures such as cytoskeletal filaments that are birefringent. By combing ing standard polarization microscopy with video enhancement techniques it has been possible to image single filaments. In this case, however, filament intensity depends on the orientation of the polarizer and analyzer with respect to the specimen.

Video Graphics and High-Voltage Electron Microscopy For Analysis of Microtubule Distributions In Fixed Cells

1990 ◽  
Vol 48 (1) ◽  
pp. 524-525
Author(s):  
Richard Mcintosh ◽  
David Mastronarde ◽  
Kent McDonald ◽  
Rubai Ding
Keyword(s):  
Electron Microscopy ◽  
Cross Sections ◽  
Cell Shape ◽  
Video Camera ◽  
Computer Memory ◽  
High Voltage Electron Microscopy ◽  
Thin Sections ◽  
Voltage Electron ◽  
Morphogenetic Event ◽  
Set Of Points

Microtubules (MTs) are cytoplasmic polymers whose dynamics have an influence on cell shape and motility. MTs influence cell behavior both through their growth and disassembly and through the binding of enzymes to their surfaces. In either case, the positions of the MTs change over time as cells grow and develop. We are working on methods to determine where MTs are at different times during either the cell cycle or a morphogenetic event, using thin and thick sections for electron microscopy and computer graphics to model MT distributions.One approach is to track MTs through serial thin sections cut transverse to the MT axis. This work uses a video camera to digitize electron micrographs of cross sections through a MT system and create image files in computer memory. These are aligned and corrected for relative distortions by using the positions of 8 - 10 MTs on adjacent sections to define a general linear transformation that will align and warp adjacent images to an optimum fit. Two hundred MT images are then used to calculate an “average MT”, and this is cross-correlated with each micrograph in the serial set to locate points likely to correspond to MT centers. This set of points is refined through a discriminate analysis that explores each cross correlogram in the neighborhood of every point with a high correlation score.

Characterization of a Motile Cellular Domain Arising During the Amoebo-Flagellate Transformation of Physarum Polycephalum

1980 ◽  
Vol 38 ◽  
pp. 552-553
Author(s):  
K.I. Pagh ◽  
M.R. Adelman
Keyword(s):  
Cell Shape ◽  
Physarum Polycephalum ◽  
Slime Mold ◽  
Live Cells ◽  
Cellular Domain

Unicellular amoebae of the slime mold Physarum polycephalum undergo marked changes in cell shape and motility during their conversion into flagellate swimming cells (l). To understand the processes underlying motile activities expressed during the amoebo-flagellate transformation, we have undertaken detailed investigations of the organization, formation and functions of subcellular structures or domains of the cell which are hypothesized to play a role in movement. One focus of our studies is on a structure, termed the “ridge” which appears as a flattened extension of the periphery along the length of transforming cells (Fig. 1). Observations of live cells using Nomarski optics reveal two types of movement in this region:propagation of undulations along the length of the ridge and formation and retraction of filopodial projections from its edge. The differing activities appear to be associated with two characteristic morphologies, illustrated in Fig. 1.

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