Simultaneous determination of1H-1H and1H-13C residual dipolar couplings in a chiral liquid crystal solvent using a natural abundance HSQC experiment

2005 ◽  
Vol 43 (7) ◽  
pp. 512-519 ◽  
Author(s):  
Vasilios M. Marathias ◽  
Igor Goljer ◽  
Alvin C. Bach II
Keyword(s):  
Liquid Crystal ◽  
Simultaneous Determination ◽  
Residual Dipolar Couplings ◽  
Natural Abundance ◽  
Dipolar Couplings ◽  
Chiral Liquid Crystal

Lyotropic liquid crystal to measure residual dipolar couplings in dimethyl sulfoxide based on modified cellulose nanocrystals

2020 ◽  
Vol 44 (11) ◽  
pp. 4262-4265 ◽  
Author(s):  
Zong-Kai Ma ◽  
Xiao-Yang Han ◽  
Han Liu ◽  
Jia-Cheng Ji ◽  
Si-Yong Qin ◽  
...  
Keyword(s):  
Liquid Crystal ◽  
Dimethyl Sulfoxide ◽  
Cellulose Nanocrystals ◽  
Residual Dipolar Couplings ◽  
Organic Molecules ◽  
Dipolar Couplings ◽  
Lyotropic Liquid Crystal ◽  
Modified Cellulose

A novel lyotropic liquid crystal was developed for the measurement of RDCs of organic molecules with no background signals.

Simultaneous Determination of Protein Backbone Structure and Dynamics from Residual Dipolar Couplings

2006 ◽  
Vol 128 (47) ◽  
pp. 15100-15101 ◽  
Author(s):  
Guillaume Bouvignies ◽  
Phineus Markwick ◽  
Rafael Brüschweiler ◽  
Martin Blackledge
Keyword(s):  
Simultaneous Determination ◽  
Residual Dipolar Couplings ◽  
Dipolar Couplings ◽  
Protein Backbone ◽  
Structure And Dynamics ◽  
Backbone Structure

REDOR recoupling in polymer-stabilized liquid crystals undergoing MAS — Two-dimensional NMR applications with strongly aligned proteins

2008 ◽  
Vol 86 (6) ◽  
pp. 608-615 ◽  
Author(s):  
Jean-François Trempe ◽  
Ekaterina Pomerantseva ◽  
Kalle Gehring
Keyword(s):  
Liquid Crystal ◽  
Residual Dipolar Couplings ◽  
Pulse Sequence ◽  
Magic Angle Spinning ◽  
Dipolar Couplings ◽  
Magic Angle ◽  
Two Dimensional ◽  
Pf1 Phage ◽  
Angle Spinning ◽  
Polymer Stabilized

We recently introduced a technique, termed SAD-REDOR, for recoupling residual dipolar couplings in a single-domain polymer-stabilized liquid crystal (PSLC) undergoing magic-angle spinning (MAS). The experiment was demonstrated with 1H–15N dipolar couplings in a small molecule. Here, we show the applicability of the technique to a strongly aligned protein and we describe a novel two-dimensional experiment to generate in-phase and anti-phase (IPAP) doublets in the 1H dimension of an HSQC NMR spectrum. This pulse sequence, SAD-IPAP, was tested on a sample of 15N-labeled ubiquitin (5 mmol/L) in a polyacrylamide-stabilized Pf1 phage liquid crystal (20 mg/mL). 15N–1H residual dipolar couplings (RDCs) were measured with the SAD-IPAP pulse sequence at spinning speeds of 1000 and 1250 Hz. RDSs were also measured using the conventional HSQC-IPAP method in a sample of 15N-ubiquitin dissolved in a solution of Pf1 phage (1 mg/mL). The resulting RDCs were fitted to the solution structure of ubiquitin to estimate the alignment tensor and to determine the accuracy of the measured couplings. The results highlight the benefits of SAD-REDOR for the measurement of RDCs in strongly aligned macromolecules.Key words: residual dipolar couplings (RDCs), polymer-stabilized liquid crystals (PSLCs), rotational echo double resonance (REDOR) recoupling, magic-angle spinning (MAS), ubiquitin, biomolecular nuclear magnetic resonance (NMR), proteins.

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