Neurostimulation success rate of repetitive‐pulse focused ultrasound in an In Vivo Giant Axon Model: An acoustic parametric study

2021 ◽  
Author(s):  
Jérémy Vion‐Bailly ◽  
Ivan M. Suarez‐Castellanos ◽  
Jean‐Yves Chapelon ◽  
Alexandre Carpentier ◽  
W. Apoutou N'Djin
Keyword(s):  
Success Rate ◽  
Parametric Study ◽  
Focused Ultrasound ◽  
Giant Axon ◽  
Axon Model

scholarly journals A causal study of the phenomenon of ultrasound neurostimulation applied to an in vivo invertebrate nervous model

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jérémy Vion-Bailly ◽  
W. Apoutou N’Djin ◽  
Ivan Mauricio Suarez Castellanos ◽  
Jean-Louis Mestas ◽  
Alexandre Carpentier ◽  
...  
Keyword(s):  
Success Rate ◽  
Focused Ultrasound ◽  
Pulse Repetition Frequency ◽  
Pulse Amplitude ◽  
Cellular Level ◽  
Acoustic Parameters ◽  
Giant Axons ◽  
Ultrasound Stimulation ◽  
Early Results

Abstract Focused ultrasound are considered to be a promising tool for the treatment of neurological conditions, overcoming the limitations of current neurostimulation techniques in terms of spatial resolution and invasiveness. Much evidence to support the feasibility of ultrasound activation of neurons at the systemic level has already been provided, but to this day, the biophysical mechanisms underlying ultrasound neurostimulation are still widely unknown. In order to be able to establish a clear and robust causality between acoustic parameters of the excitation and neurobiological characteristics of the response, it is necessary to work at the cellular level, or alternatively on very simple animal models. The study reported here responds to three objectives. Firstly, to propose a simple nervous model for the study of the ultrasound neurostimulation phenomenon, associated with a clear and simple experimental protocol. Secondly, to compare the characteristics of this model’s nervous response to ultrasound neurostimulation with its nervous response to mechanical and electrical stimulation. Thirdly, to study the role played by certain acoustic parameters in the success rate of the phenomenon of ultrasound stimulation. The feasibility of generating action potentials (APs) in the giant axons of an earthworm’s ventral nerve cord, using pulsed ultrasound stimuli (f = 1.1 MHz, Ncycles = 175–1150, PRF = 25–125 Hz, Npulses = 20, PA = 2.5–7.3 MPa), was demonstrated. The time of generation (TOG) of APs associated with ultrasound stimulation was found to be significantly shorter and more stable than the TOG associated with mechanical stimulation (p < 0.001). By applying a causal approach to interpret the results of this study, it was concluded that, in this model, the nervous response to focused ultrasound is initiated along the afferent neurons, in between the mechanosensors and the synaptic connections with the giant axons. Additionally, early results are provided, highlighting a trend for the success rate of ultrasound neurostimulation and number of APs triggered per response to increase with increasing pulse repetition frequency (p < 0.05 and p < 0.001, respectively), increasing pulse duration and increasing pulse amplitude.

Ultrasound-based Safety Assessment during Moving Organ Tracking Towards In Vivo Focused Ultrasound Therapy

2019 ◽  
Author(s):  
A Mariani ◽  
◽  
L Morchi ◽  
A Diodato ◽  
A Cafarelli ◽  
...  
Keyword(s):  
Safety Assessment ◽  
Focused Ultrasound ◽  
Ultrasound Therapy

scholarly journals Single-cell mapping of focused ultrasound-transfected brain

Gene Therapy ◽  
2021 ◽  
Author(s):  
A. S. Mathew ◽  
C. M. Gorick ◽  
R. J. Price
Keyword(s):  
Single Cell ◽  
Focused Ultrasound ◽  
Cell Types ◽  
Brain Cell ◽  
Therapeutic Modality ◽  
Full Potential ◽  
Stress Genes ◽  
Multiple Cell ◽  
Differential Gene

AbstractGene delivery via focused ultrasound (FUS) mediated blood-brain barrier (BBB) opening is a disruptive therapeutic modality. Unlocking its full potential will require an understanding of how FUS parameters (e.g., peak-negative pressure (PNP)) affect transfected cell populations. Following plasmid (mRuby) delivery across the BBB with 1 MHz FUS, we used single-cell RNA-sequencing to ascertain that distributions of transfected cell types were highly dependent on PNP. Cells of the BBB (i.e., endothelial cells, pericytes, and astrocytes) were enriched at 0.2 MPa PNP, while transfection of cells distal to the BBB (i.e., neurons, oligodendrocytes, and microglia) was augmented at 0.4 MPa PNP. PNP-dependent differential gene expression was observed for multiple cell types. Cell stress genes were upregulated proportional to PNP, independent of cell type. Our results underscore how FUS may be tuned to bias transfection toward specific brain cell types in vivo and predict how those cells will respond to transfection.

scholarly journals Thalamic Connectivity in Patients with Essential Tremor Treated with MR Imaging–guided Focused Ultrasound: In Vivo Fiber Tracking by Using Diffusion-Tensor MR Imaging

Radiology ◽  
2014 ◽  
Vol 272 (1) ◽  
pp. 202-209 ◽  
Author(s):  
Max Wintermark ◽  
Diane S. Huss ◽  
Binit B. Shah ◽  
Nicholas Tustison ◽  
T. Jason Druzgal ◽  
...  
Keyword(s):  
Mr Imaging ◽  
Essential Tremor ◽  
Focused Ultrasound ◽  
Diffusion Tensor ◽  
Fiber Tracking ◽  
Diffusion Tensor Mr Imaging

In vivo MRI visualization of release from liposomes triggered by local application of pulsed low-intensity non-focused ultrasound

2014 ◽  
Vol 10 (5) ◽  
pp. e901-e904 ◽  
Author(s):  
Silvia Rizzitelli ◽  
Pierangela Giustetto ◽  
Cinzia Boffa ◽  
Daniela Delli Castelli ◽  
Juan Carlos Cutrin ◽  
...  
Keyword(s):  
Focused Ultrasound ◽  
Local Application ◽  
Low Intensity

scholarly journals Trans-abdominal in vivo placental vessel occlusion using High Intensity Focused Ultrasound

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Caroline J. Shaw ◽  
Ian Rivens ◽  
John Civale ◽  
Kimberley J. Botting ◽  
Gail ter Haar ◽  
...  
Keyword(s):  
High Intensity ◽  
Focused Ultrasound ◽  
High Intensity Focused Ultrasound ◽  
Vessel Occlusion ◽  
Placental Vessel
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