Syringaresinol Protects against Type 1 Diabetic Cardiomyopathy by Alleviating Inflammation Responses, Cardiac Fibrosis, and Oxidative Stress

2020 ◽  
Vol 64 (18) ◽  
pp. 2000231
Author(s):  
Guangru Li ◽  
Lei Yang ◽  
Lifeng Feng ◽  
Jiu Yang ◽  
Yafei Li ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetic Cardiomyopathy ◽  
Cardiac Fibrosis ◽  
And Oxidative Stress ◽  
Inflammation Responses

Abstract 219: NAD Redox Imbalance Accelerates Diabetic Cardiomyopathy via Protein Acetylation and Oxidative Stress

2020 ◽  
Vol 127 (Suppl_1) ◽  
Author(s):  
Ying Ann Chiao ◽  
Christine Light ◽  
Xiaojian Shi ◽  
Rong Tian ◽  
Junichi Sadoshima ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mouse Models ◽  
Diabetic Cardiomyopathy ◽  
Cardiac Fibrosis ◽  
Redox Balance ◽  
Protein Acetylation ◽  
Redox Imbalance ◽  
Nadh Ratio ◽  
Systolic And Diastolic Function ◽  
And Oxidative Stress

Diabetes is long linked to lowered NAD/NADH ratio, aka NAD redox imbalance, but its causal role to diabetic cardiomyopathy is not established. We used mouse models with latent decrease in cardiac NAD/NADH ratio (cardiac-specific Ndufs4-KO, cKO) and elevated cardiac NAD levels to directly test whether cardiac NAD redox imbalance accelerates diabetic cardiomyopathy. Control and cKO mice were subjected to 8-week T1D stress, and longitudinal cardiac function was measured by echocardiography. Accelerated declines in systolic and diastolic function were observed in T1D cKO mice. Insulin depletion and hyperglycemia were similar in T1D control and T1D cKO mice, and serum metabolomic analyses showed unchanged aqueous and lipid metabolite levels. These metabolite results suggested that T1D control and cKO hearts were stressed under similar diabetic conditions. Importantly, elevation of cardiac NAD levels to attenuate NAD redox imbalance mitigated the accelerated functional declines in T1D cKO hearts. The data from mouse models with manipulated NAD redox states suggested that NAD redox imbalance accelerates diabetic cardiomyopathy. Cardiac fibrosis levels were not different in T1D control and cKO hearts, while transcript levels of fibrotic genes, including Adamts proteinases, integrins, laminins, matrix metalloproteinases and collagens, also showed no difference. Therefore, the accelerated functional declines in T1D cKO hearts are not due to altered extracellular matrix environment, but are rather due to cardiomyocyte dysfunction. We next determined whether the accelerated cardiac dysfunction is mediated via protein acetylation and oxidative stress. NAD-dependent global protein acetylation and inhibitory acetylation of superoxide dismutase 2 were elevated in T1D cKO hearts. Inhibition of SOD2 concomitantly promoted elevation of protein oxidation levels in T1D cKO hearts. The results suggested that NAD redox balance-dependent protein acetylation regulates oxidative stress to promote diabetic cardiomyopathy.

scholarly journals Deletion of RasGRF1 Attenuated Interstitial Fibrosis in Streptozotocin-Induced Diabetic Cardiomyopathy in Mice through Affecting Inflammation and Oxidative Stress

2018 ◽  
Vol 19 (10) ◽  
pp. 3094 ◽  
Author(s):  
Tzu-Hsien Tsai ◽  
Cheng-Jei Lin ◽  
Sarah Chua ◽  
Sheng-Ying Chung ◽  
Shyh-Ming Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diastolic Function ◽  
Diabetic Cardiomyopathy ◽  
Cardiac Fibrosis ◽  
Interstitial Fibrosis ◽  
Cardiac Inflammation ◽  
Matrix Deposition ◽  
Stress Markers ◽  
Group 2 ◽  
And Oxidative Stress

Background: Diabetic cardiomyopathy (DCM) is characterized by cardiac fibrosis and stiffness, which often develops into heart failure. This study investigated the role of Ras protein-specific guanine nucleotide releasing factor 1 (RasGRF1) in the development of DCM. Methods: Forty-eight mice were divided into four groups (n = 12 per group): Group 1: Wild-type (WT) mice, Group 2: RasGRF1 deficiency (RasGRF1−/−) mice. Group 3: Streptozotocin (STZ)-induced diabetic WT mice, Group 4: STZ-induced diabetic RasGRF1−/− mice. Myocardial functions were assessed by cardiac echography. Heart tissues from all of the mice were investigated for cardiac fibrosis, inflammation, and oxidative stress markers. Results: Worse impaired diastolic function with elevation serum interleukin (IL)-6 was found in the diabetic group compared with the non-diabetic groups. Serum IL-6 levels were found to be elevated in the diabetic compared with the non-diabetic groups. However, the diabetic RasGRF1−/− mice exhibited lower serum IL-6 levels and better diastolic function than the diabetic WT mice. The diabetic RasGRF1−/− mice were associated with reduced cardiac inflammation, which was shown by lower invading inflammation cells, lower expression of matrix metalloproteinase 9, and less chemokines compared to the diabetic WT mice. Furthermore, less oxidative stress as well as extracellular matrix deposition leading to a reduction in cardiac fibrosis was also found in the diabetic RasGRF1−/− mice compared with the diabetic WT mice. Conclusion: The deletion of RasGRF1 attenuated myocardial fibrosis and improved cardiac function in diabetic mice through inhibiting inflammation and oxidative stress.

scholarly journals Beneficial Effect of Aqueous Garlic Extract on Inflammation and Oxidative Stress Status in the Kidneys of Type 1 Diabetic Rats

2016 ◽  
Vol 32 (3) ◽  
pp. 329-336 ◽  
Author(s):  
Abolfazl Nasiri ◽  
Nasrin Ziamajidi ◽  
Roghayeh Abbasalipourkabir ◽  
Mohammad Taghi Goodarzi ◽  
Massoud Saidijam ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Beneficial Effect ◽  
Diabetic Rats ◽  
Garlic Extract ◽  
Oxidative Stress Status ◽  
And Oxidative Stress

Type 1 diabetes and oxidative stress markers

2021 ◽  
Vol 242 ◽  
pp. 174
Author(s):  
Widad Sobhi ◽  
Rania Derguine ◽  
Saliha Boucheffa ◽  
Abdelhalim Khenchouche ◽  
Nada Boutrid ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Type 1 Diabetes ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
And Oxidative Stress

Diabetic Cardiomyopathy and Oxidative Stress

2014 ◽  
pp. 25-32 ◽  
Author(s):  
Somasundaram Arumugam ◽  
Vengadeshprabhu Karuppagounder ◽  
Rajarajan A. Thandavarayan ◽  
Vigneshwaran Pitchaimani ◽  
Hirohito Sone ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetic Cardiomyopathy ◽  
And Oxidative Stress
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