In Vitro Gut Metabolism of [U-13C]-Quinic Acid, The Other Hydrolysis Product of Chlorogenic Acid

2018 ◽  
Vol 62 (22) ◽  
pp. 1800396 ◽  
Author(s):  
Martine Naranjo Pinta ◽  
Ivan Montoliu ◽  
Anna-Marja Aura ◽  
Tuulikki Seppänen-Laakso ◽  
Denis Barron ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Hydrolysis Product ◽  
Quinic Acid ◽  
The Other ◽  
Gut Metabolism

scholarly journals Front cover: In Vitro Gut Metabolism of [U-13 C]-Quinic Acid, The Other Hydrolysis Product of Chlorogenic Acid

2018 ◽  
Vol 62 (22) ◽  
pp. 1870094
Author(s):  
Martine Naranjo Pinta ◽  
Ivan Montoliu ◽  
Anna-Marja Aura ◽  
Tuulikki Seppänen-Laakso ◽  
Denis Barron ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Hydrolysis Product ◽  
Quinic Acid ◽  
The Other ◽  
Front Cover ◽  
Gut Metabolism

scholarly journals Relevance and Standardization of In Vitro Antioxidant Assays: ABTS, DPPH, and Folin–Ciocalteu

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Helena Abramovič ◽  
Blaž Grobin ◽  
Nataša Poklar Ulrih ◽  
Blaž Cigić
Keyword(s):  
Ascorbic Acid ◽  
Chlorogenic Acid ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
Sulfonic Acid ◽  
Epigallocatechin Gallate ◽  
The Other ◽  
Oh Groups ◽  
Standard Compound

Trolox, gallic acid, chlorogenic acid, caffeic acid, catechin, epigallocatechin gallate, and ascorbic acid are antioxidants used as standards for reaction with chromogenic radicals, 2,2-diphenyl-1-picrylhydrazyl (DPPH⋅) and 2,2′-azino-bis-3-ethylbenzotiazolin-6-sulfonic acid (ABTS⋅+), and Folin–Ciocalteu (FC) reagent. The number of exchanged electrons has been analyzed as function of method and solvent. A majority of compounds exchange more electrons in FC assay than in ABTS and DPPH assays. In reaction with chromogenic radicals, the largest number of electrons was exchanged in buffer (pH 7.4) and the lowest reactivity was in methanol (DPPH) and water (ABTS). At physiological pH, the number of exchanged electrons of polyphenols exceeded the number of OH groups, pointing to the important contribution of partially oxidized antioxidants, formed in the course of reaction, to the antioxidant potential. For Trolox, small impact on the number of exchanged electrons was observed, confirming that it is more suitable as a standard compound than the other antioxidants.

Isolation and Characterization of a Chlorogenic Acid Esterase from Aspergillus niger

1980 ◽  
Vol 35 (3-4) ◽  
pp. 209-212 ◽  
Author(s):  
B. Schöbel ◽  
W. Pollmann
Keyword(s):  
Enzyme Activity ◽  
Chlorogenic Acid ◽  
Divalent Cations ◽  
Hydrolysis Product ◽  
The Other ◽  
Temperature Optimum ◽  
Pig Liver ◽  
Isolation And Characterization

Abstract The isolation and characterization of a specific chlorogenic acid esterase is described. The enzyme activity is measured by determination of the hydrolysis product caffeic acid. The enzyme had been concentrated by means of ultrafiltration and column-chromatography. The pH- and temperature optimum were 6.5 and 45 °C respectively. Divalent cations were not required for the enzyme activity. As other esterases, this enzyme is inhibited by di-isopropyl-phosphorofluoridate. The Km-value is 0.70 mᴍ chlorogenic acid, the molecular weight 240000. The described enzyme is specific for chlorogenic acid. On the other hand a typical unspecific esterase like the pig liver esterases does not split chloro­genic acid. The isoelectric focusing reveals several isoenzymes of chlorogenase within a pI-range of 4.0-4.5.

scholarly journals Selective overexpression of the QUTE gene encoding catabolic 3-dehydroquinase in multicopy transformants of Aspergillus nidulans

1990 ◽  
Vol 265 (2) ◽  
pp. 337-342 ◽  
Author(s):  
R K Beri ◽  
S Grant ◽  
C F Roberts ◽  
M Smith ◽  
A R Hawkins
Keyword(s):  
Chlorogenic Acid ◽  
Aspergillus Nidulans ◽  
Gene Cluster ◽  
Quinic Acid ◽  
The Other ◽  
Gene Encoding ◽  
Repressor Gene ◽  
Catabolic Enzymes ◽  
Multiple Copies

The three enzymes necessary to catabolize quinate to protocatechuate are inducible by quinic acid, and transcription of their corresponding genes is controlled by the action of a positively acting activator gene and a negatively acting repressor gene. Transformed strains of Aspergillus nidulans containing multiple copies of the activator gene (QUTA) but single copies of the other QUT genes retain normal regulation of the gene cluster and do not show any overexpression of the three quinic acid catabolic enzymes. Transformed strains containing equal multiple copies of the activator gene (QUTA) and QUTE (encoding catabolic 3-dehydroquinase), but single copies of the other QUT genes, retain normal regulation of the QUT gene cluster, but selectively overexpress the QUTE gene upon quinic acid induction. Data are presented that strongly suggested that the gene QUTG, which is physically located within the QUT gene cluster and for which no function has been identified, is not required for expression of the gene cluster and does not encode a chlorogenic acid esterase.

Demonstrating the involvement of an active efflux mechanism in the intestinal absorption of chlorogenic acid and quinic acid using a Caco-2 bidirectional permeability assay

2021 ◽  
Author(s):  
Olivier Mortelé ◽  
Jennifer Jörissen ◽  
Irina Spacova ◽  
Sarah Lebeer ◽  
Alexander L. N. van Nuijs ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Intestinal Absorption ◽  
Quinic Acid ◽  
Active Efflux ◽  
Efflux Mechanism ◽  
Permeability Assay

The intestinal absorption of chlorogenic acid and quinic acid was investigated using an in vitro bidirectional Caco-2 permeability assay and LC-MS/MS.

Caffeic and chlorogenic acids inhibit key enzymes linked to type 2 diabetes (in vitro): a comparative study

2015 ◽  
Vol 26 (2) ◽  
Author(s):  
Ganiyu Oboh ◽  
Odunayo M. Agunloye ◽  
Stephen A. Adefegha ◽  
Ayodele J. Akinyemi ◽  
Adedayo O. Ademiluyi
Keyword(s):  
Type 2 Diabetes ◽  
Chlorogenic Acid ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
Quinic Acid ◽  
Inhibitory Effect ◽  
Dependent Manner ◽  
Key Enzymes

AbstractChlorogenic acid is a major phenolic compound that forms a substantial part of plant foods and is an ester of caffeic acid and quinic acid. However, the effect of the structures of both chlorogenic and caffeic acids on their antioxidant and antidiabetic potentials have not been fully understood. Thus, this study sought to investigate and compare the interaction of caffeic acid and chlorogenic acid with α-amylase and α-glucosidase (key enzymes linked to type 2 diabetes) activities in vitro.The inhibitory effect of the phenolic acids on α-amylase and α-glucosidase activities was evaluated. Thereafter, their antioxidant activities as typified by their 1,1-diphenyl-2 picrylhydrazyl radical scavenging ability and ferric reducing antioxidant properties were determined.The results revealed that both phenolic acids inhibited α-amylase and α-glucosidase activities in a dose-dependent manner (2–8 μg/mL). However, caffeic acid had a significantly (p<0.05) higher inhibitory effect on α-amylase [ICThe esterification of caffeic acid with quinic acid, producing chlorogenic acid, reduces their ability to inhibit α-amylase and α-glucosidase activities. Thus, the inhibition of α-amylase and α-glucosidase activities by the phenolic acids could be part of the possible mechanism by which the phenolic acids exert their antidiabetic effects.

Simulated Gastrointestinal Biotransformation of Chlorogenic Acid, Flavonoids, Flavonolignans and Triterpenoid Saponins in Cecropia obtusifolia Leaf Extract

Planta Medica ◽  
2020 ◽  
Author(s):  
Andrés Rivera-Mondragón ◽  
Laura Peeters ◽  
Anastasiader Auwera Van ◽  
Annelies Breynaert ◽  
Catherina Caballero-George ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Leaf Extract ◽  
Chemical Constituents ◽  
The Other ◽  
Bioactive Metabolites ◽  
Triterpenoid Saponins ◽  
Cecropia Obtusifolia ◽  
Machine Learning Model ◽  
Automated Machine Learning

AbstractIt is well known that biotransformation processes in the human body are crucial to form potentially bioactive metabolites from particular classes of natural products. However, little research has been conducted concerning the bioavailability of polyphenols, especially in the colon. The gastrointestinal stability and colonic biotransformation of the crude extract of the leaves of Cecropia obtusifolia, rich in flavone C-glycosides, was investigated under in vitro conditions, and the processing and interpretation of results were facilitated by using an automated machine learning model. This investigation revealed that flavone C-glycosides and flavonolignans from C. obtusifolia were stable throughout their passage in the simulated gastrointestinal tract including the colon phase. On the other hand, the colon bacteria extensively metabolized chlorogenic acid, flavonol, and triterpenoid O-glycosides. This investigation revealed that the colonic microbiota has an important role in the biotransformation of some chemical constituents of this extract.

Anti-hepatitis B virus activity of chlorogenic acid, quinic acid and caffeic acid in vivo and in vitro

2009 ◽  
Vol 83 (2) ◽  
pp. 186-190 ◽  
Author(s):  
Gui-Feng Wang ◽  
Li-Ping Shi ◽  
Yu-Dan Ren ◽  
Qun-Fang Liu ◽  
Hou-Fu Liu ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Chlorogenic Acid ◽  
Caffeic Acid ◽  
Quinic Acid ◽  
Virus Activity ◽  
B Virus

Enhancement of ADP-induced Platelet Aggregation by Adrenaline in Vivo and Its Prevention

1973 ◽  
Vol 29 (02) ◽  
pp. 490-498 ◽  
Author(s):  
Hiroh Yamazaki ◽  
Itsuro Kobayashi ◽  
Tadahiro Sano ◽  
Takio Shimamoto
Keyword(s):  
Platelet Count ◽  
Platelet Aggregation ◽  
Platelet Rich Plasma ◽  
The Other ◽  
Endothelial Surface ◽  
Important Interaction ◽  
Blood Coagulability ◽  
The Difference

SummaryThe authors previously reported a transient decrease in adhesive platelet count and an enhancement of blood coagulability after administration of a small amount of adrenaline (0.1-1 µg per Kg, i. v.) in man and rabbit. In such circumstances, the sensitivity of platelets to aggregation induced by ADP was studied by an optical density method. Five minutes after i. v. injection of 1 µg per Kg of adrenaline in 10 rabbits, intensity of platelet aggregation increased to 115.1 ± 4.9% (mean ± S. E.) by 10∼5 molar, 121.8 ± 7.8% by 3 × 10-6 molar and 129.4 ± 12.8% of the value before the injection by 10”6 molar ADP. The difference was statistically significant (P<0.01-0.05). The above change was not observed in each group of rabbits injected with saline, 1 µg per Kg of 1-noradrenaline or 0.1 and 10 µg per Kg of adrenaline. Also, it was prevented by oral administration of 10 mg per Kg of phenoxybenzamine or propranolol or aspirin or pyridinolcarbamate 3 hours before the challenge. On the other hand, the enhancement of ADP-induced platelet aggregation was not observed in vitro, when 10-5 or 3 × 10-6 molar and 129.4 ± 12.8% of the value before 10∼6 molar ADP was added to citrated platelet rich plasma (CPRP) of rabbit after incubation at 37°C for 30 second with 0.01, 0.1, 1, 10 or 100 µg per ml of adrenaline or noradrenaline. These results suggest an important interaction between endothelial surface and platelets in connection with the enhancement of ADP-induced platelet aggregation by adrenaline in vivo.

ALDOSTERONE STIMULATION IN VITRO

1965 ◽  
Vol 50 (2) ◽  
pp. 301-309 ◽  
Author(s):  
Jürg Müller
Keyword(s):  
Ammonium Chloride ◽  
Human Urine ◽  
Incubation Medium ◽  
The Other ◽  
Ammonium Ions ◽  
Response Curves ◽  
Urine Extract ◽  
Similar Dose ◽  
Aldosterone Production

ABSTRACT An extract of human urine, which was previously shown to stimulate aldosterone production by rat adrenal sections, was further purified. Evidence was obtained that its aldosterone-stimulating effect was due to the presence of ammonium ions. Addition of ammonium chloride and of urine extract to the incubation medium caused identical increases in aldosterone production in vitro. In addition to ammonium ions, rubidium and caesium ions also stimulated aldosterone production up to 250% that of control values without a significant effect on corticosterone production. Similar dose-response curves were obtained when increasing concentrations of potassium, ammonium, rubidium and caesium ions were tested. Aldosterone production was maximal at concentrations of 7 mval/1 and was significantly lower at higher concentrations. When ammonium chloride and ACTH were simultaneously added to the incubation medium, the production of aldosterone and of corticosterone was lower than with ACTH alone. On the other hand, the stimulating activity on aldosterone and corticosterone production by »TPN« (NADP) and glucose-6-phosphate was enhanced by the simultaneous addition of ammonium chloride.

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