scholarly journals Exosomal MicroRNAs in Milk from Mothers Delivering Preterm Infants Survive in Vitro Digestion and Are Taken Up by Human Intestinal Cells

2018 ◽  
Vol 62 (11) ◽  
pp. 1701050 ◽  
Author(s):  
Sarah Kahn ◽  
Yalin Liao ◽  
Xiaogu Du ◽  
Wei Xu ◽  
Jie Li ◽  
...  
Keyword(s):  
Preterm Infants ◽  
In Vitro Digestion ◽  
Intestinal Cells ◽  
Human Intestinal Cells

scholarly journals Human milk glycosaminoglycans inhibit in vitro the adhesion of Escherichia coli and Salmonella fyris to human intestinal cells

2015 ◽  
Vol 79 (4) ◽  
pp. 603-607 ◽  
Author(s):  
Giovanni V. Coppa ◽  
Bruna Facinelli ◽  
Gloria Magi ◽  
Emanuela Marini ◽  
Lucia Zampini ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Human Milk ◽  
Intestinal Cells ◽  
Human Intestinal Cells

scholarly journals Bifidobacterium longum subsp. infantis ATCC 15697 and Goat Milk Oligosaccharides Show Synergism In Vitro as Anti-Infectives against Campylobacter jejuni

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 348 ◽  
Author(s):  
Erinn M. Quinn ◽  
Helen Slattery ◽  
Dan Walsh ◽  
Lokesh Joshi ◽  
Rita M. Hickey
Keyword(s):  
Campylobacter Jejuni ◽  
Bifidobacterium Longum ◽  
Goat Milk ◽  
Host Cells ◽  
Intestinal Cells ◽  
Milk Oligosaccharides ◽  
Bifidobacterium Strain ◽  
Ht 29 ◽  
Human Intestinal Cells

Bifidobacteria are known to inhibit, compete with and displace the adhesion of pathogens to human intestinal cells. Previously, we demonstrated that goat milk oligosaccharides (GMO) increased the attachment of Bifidobacterium longum subsp. infantis ATCC 15697 to intestinal cells in vitro. In this study, we aimed to exploit this effect as a mechanism for inhibiting pathogen association with intestinal cells. We examined the synergistic effect of GMO-treated B. infantis on preventing the attachment of a highly invasive strain of Campylobacter jejuni to intestinal HT-29 cells. The combination decreased the adherence of C. jejuni to the HT-29 cells by an average of 42% compared to the control (non-GMO treated B. infantis). Increasing the incubation time of the GMO with the Bifidobacterium strain resulted in the strain metabolizing the GMO, correlating with a subsequent 104% increase in growth over a 24 h period when compared to the control. Metabolite analysis in the 24 h period also revealed increased production of acetate, lactate, formate and ethanol by GMO-treated B. infantis. Statistically significant changes in the GMO profile were also demonstrated over the 24 h period, indicating that the strain was digesting certain structures within the pool such as lactose, lacto-N-neotetraose, lacto-N-neohexaose 3′-sialyllactose, 6′-sialyllactose, sialyllacto-N-neotetraose c and disialyllactose. It may be that early exposure to GMO modulates the adhesion of B. infantis while carbohydrate utilisation becomes more important after the bacteria have transiently colonised the host cells in adequate numbers. This study builds a strong case for the use of synbiotics that incorporate oligosaccharides sourced from goat′s milk and probiotic bifidobacteria in functional foods, particularly considering the growing popularity of formulas based on goat milk.

In vitro exposure of human intestinal cells to iron phosphate nanoparticles indicate no direct cytotoxicity

2013 ◽  
Vol 221 ◽  
pp. S242
Author(s):  
Lea von Moos ◽  
Ioannis Trantakis ◽  
Peter Rast ◽  
Florentine Hilty-Vancura ◽  
Michael Zimmermann ◽  
...  
Keyword(s):  
Iron Phosphate ◽  
Intestinal Cells ◽  
In Vitro Exposure ◽  
Direct Cytotoxicity ◽  
Human Intestinal Cells

Lactococcus lactis subsp. cremoris strain JFR1 attenuates Salmonella adhesion to human intestinal cells in vitro

2016 ◽  
Vol 90 ◽  
pp. 147-153 ◽  
Author(s):  
Justina Su Zhang ◽  
Anilda Guri ◽  
Milena Corredig ◽  
Rocio Morales-Rayas ◽  
Ashraf Hassan ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Intestinal Cells ◽  
Lactococcus Lactis Subsp ◽  
Human Intestinal Cells

Cytotoxicity of coffee in human intestinal cells in vitro and its inhibition by peroxidase

1992 ◽  
Vol 6 (5) ◽  
pp. 417-421 ◽  
Author(s):  
S.K. Itagaki ◽  
T. Kobayashi ◽  
Y. Kitagawa ◽  
S. Iwata ◽  
Y. Suwa ◽  
...  
Keyword(s):  
Intestinal Cells ◽  
Human Intestinal Cells

Impact of food components during in vitro digestion of silver nanoparticles on cellular uptake and cytotoxicity in intestinal cells

2015 ◽  
Vol 396 (11) ◽  
pp. 1255-1264 ◽  
Author(s):  
Dajana Lichtenstein ◽  
Johanna Ebmeyer ◽  
Patrick Knappe ◽  
Sabine Juling ◽  
Linda Böhmert ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cellular Uptake ◽  
In Vitro Digestion ◽  
Intestinal Cells ◽  
Digestion Process ◽  
X Ray Scattering ◽  
Food Components ◽  
Uptake Studies

Abstract Because of the rising application of nanoparticles in food and food-related products, we investigated the influence of the digestion process on the toxicity and cellular uptake of silver nanoparticles for intestinal cells. The main food components – carbohydrates, proteins and fatty acids – were implemented in an in vitro digestion process to simulate realistic conditions. Digested and undigested silver nanoparticle suspensions were used for uptake studies in the well-established Caco-2 model. Small-angle X-ray scattering was used to estimate particle core size, size distribution and stability in cell culture medium. Particles proved to be stable and showed radii from 3.6 to 16.0 nm. Undigested particles and particles digested in the presence of food components were comparably taken up by Caco-2 cells, whereas the uptake of particles digested without food components was decreased by 60%. Overall, these findings suggest that in vivo ingested poly (acrylic acid)-coated silver nanoparticles may reach the intestine in a nanoscaled form even if enclosed in a food matrix. While appropriate for studies on the uptake into intestinal cells, the Caco-2 model might be less suited for translocation studies. Moreover, we show that nanoparticle digestion protocols lacking food components may lead to misinterpretation of uptake studies and inconclusive results.

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