(‐)‐Epigallocatechin‐3‐gallate Ameliorates Insulin Resistance and Mitochondrial Dysfunction in HepG2 Cells: Involvement of Bmal1

2017 ◽  
Vol 61 (12) ◽  
pp. 1700440 ◽  
Author(s):  
Yashi Mi ◽  
Guoyuan Qi ◽  
Yuqi Gao ◽  
Runnan Li ◽  
Yiwen Wang ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Mitochondrial Dysfunction ◽  
Hepg2 Cells

Nascent Protein Labeling Strategy Disclosed Mitochondrial Proteomic Response in Punicalagin Intervened Insulin Resistance of HepG2 Cells

2022 ◽  
Author(s):  
Zhengyi Zhang ◽  
Mengqi Zeng ◽  
Xiao Han ◽  
Zhanwu Hou ◽  
Zhen Wang ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Mitochondrial Dysfunction ◽  
Hepg2 Cells ◽  
Metabolic Diseases ◽  
Protein Labeling ◽  
Obesity And Diabetes ◽  
The Common ◽  
Proteomic Response

Insulin Resistance (IR), as the common pathophysiological basis, is closely related to a variety of metabolic diseases, such as obesity and diabetes. IR is often accompanied with mitochondrial dysfunction which...

scholarly journals Polysaccharide Derived from Nelumbo nucifera Lotus Plumule Shows Potential Prebiotic Activity and Ameliorates Insulin Resistance in HepG2 Cells

Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1780
Author(s):  
Bao Le ◽  
Pham-Thi-Ngoc Anh ◽  
Seung-Hwan Yang
Keyword(s):  
Insulin Resistance ◽  
Hepg2 Cells ◽  
Structural Characteristics ◽  
Physicochemical Characterization ◽  
Deae Cellulose ◽  
Hot Water ◽  
Nelumbo Nucifera ◽  
Molar Ratio ◽  
Prebiotic Activity ◽  
Insulin Resistant

Polysaccharides are key bioactive compounds in lotus plumule tea, but their anti-diabetes activities remain unclear. The purpose of this study was to investigate the prebiotic activities of a novel polysaccharide fraction from the Nelumbo nucifera lotus plumule, and to examine its regulation of glucose metabolism in insulin-resistant HepG2 cells. The N. nucifera polysaccharide (NNP) was purified after discoloration, hot water extraction, ethanol precipitation, and DEAE-cellulose chromatography to obtain purified polysaccharide fractions (NNP-2). Fourier transform infrared spectroscopy was used to analyze the main structural characteristics and functional group of NNP-2. Physicochemical characterization indicated that NNP-2 had a molecular weight of 110.47 kDa and consisted of xylose, glucose, fructose, galactose, and fucose in a molar ratio of 33.4:25.7:22.0:10.5:8.1. The prebiotic activity of NNP-2 was demonstrated in vitro using Lactobacillus and Bifidobacterium. Furthermore, NNP-2 showed bioactivity against α-glucosidase (IC50 = 97.32 µg/mL). High glucose-induced insulin-resistant HepG2 cells were used to study the effect of NNP-2 on glucose consumption, and the molecular mechanism of the insulin transduction pathway was studied using RT-qPCR. NNP-2 could improve insulin resistance by modulating the IRS1/PI3K/Akt pathway in insulin-resistant HepG2 cells. Our data demonstrated that the Nelumbo nucifera polysaccharides are potential sources for nutraceuticals, and we propose functional food developments from the bioactive polysaccharides of N. nucifera for the management of diabetes.

Sign in / Sign up

Export Citation Format

Share Document