Enhanced M2 macrophage polarization in high n-3 polyunsaturated fatty acid transgenic mice fed a high-fat diet

2016 ◽  
Vol 60 (11) ◽  
pp. 2481-2492 ◽  
Author(s):  
Mi-Young Song ◽  
Jie Wang ◽  
Youngyi Lee ◽  
Juhyung Lee ◽  
Keun-Sang Kwon ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Transgenic Mice ◽  
Polyunsaturated Fatty Acid ◽  
High Fat Diet ◽  
Macrophage Polarization ◽  
M2 Macrophage ◽  
High Fat ◽  
M2 Macrophage Polarization

scholarly journals Effects of Steady Low-Intensity Exercise on High-Fat Diet Stimulated Breast Cancer Progression Via the Alteration of Macrophage Polarization

2020 ◽  
Vol 19 ◽  
pp. 153473542094967
Author(s):  
Min Kyoon Kim ◽  
Yesl Kim ◽  
SeungHwa Park ◽  
Eunju Kim ◽  
Yerin Kim ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Growth ◽  
High Fat Diet ◽  
Tumor Volume ◽  
Macrophage Polarization ◽  
M2 Macrophage ◽  
High Fat ◽  
Low Intensity ◽  
M2 Macrophage Polarization ◽  
Low Intensity Exercise

Physical inactivity and high-fat diet, especially high saturated fat containing diet are established risk factors for breast cancer that are amenable to intervention. High-fat diet has been shown to induce tumor growth and metastasis by alteration of inflammation but steady exercise has anti-tumorigenic effects. However, the mechanisms underlying the effects of physical activity on high-fat diet stimulated breast cancer initiation and progression are currently unclear. In this study, we examined how the intensity of physical activity influences high fat diet-stimulated breast cancer latency and progression outcomes, and the possible mechanisms behind these effects. Five-week-old female Balb/c mice were fed either a control diet or a high-fat diet for 8 weeks, and then 4T1 mouse mammary tumor cells were inoculated into the mammary fat pads. Exercise training occurred before tumor cell injection, and tumor latency and tumor volume were measured. Mice with a high-fat diet and low-intensity exercise (HFLE) had a longer tumor latency period, slower tumor growth, and smaller tumor volume in the final tumor assessment compared with the control, high-fat diet control (HFDC), and high-fat diet with moderate-intensity exercise (HFME) groups. Steady low- and moderate-intensity exercise had no effect on cell proliferation but induced apoptosis by activating caspase-3 through the alteration of Bcl-2, Bcl-xL, and Bax expression. Furthermore, steady exercise reduced M2 macrophage polarization in breast tumor tissue, which has been linked to tumor growth. The myokine, myostatin, reduced M2 macrophage polarization through the inhibition of the JAK-STAT signaling pathway. These results suggest that steady low-intensity exercise could delay breast cancer initiation and growth and reduce tumor volume through the induction of tumor cell apoptosis and the suppression of M2 macrophage polarization.

Opuntia ficus-indica seed attenuates hepatic steatosis and promotes M2 macrophage polarization in high-fat diet–fed mice

2016 ◽  
Vol 36 (4) ◽  
pp. 369-379 ◽  
Author(s):  
Jung-Woo Kang ◽  
Jun-Kyu Shin ◽  
Eun-Ji Koh ◽  
Hyojeong Ryu ◽  
Hyoung Ja Kim ◽  
...  
Keyword(s):  
Hepatic Steatosis ◽  
High Fat Diet ◽  
Macrophage Polarization ◽  
M2 Macrophage ◽  
High Fat ◽  
Opuntia Ficus Indica ◽  
M2 Macrophage Polarization

scholarly journals Huang-Lian-Jie-Du Decoction Attenuates Atherosclerosis and Increases Plaque Stability in High-Fat Diet-Induced ApoE-/- Mice by Inhibiting M1 Macrophage Polarization and Promoting M2 Macrophage Polarization

2021 ◽  
Vol 12 ◽  
Author(s):  
Yinhe Cai ◽  
Junmao Wen ◽  
Siwen Ma ◽  
Zhexing Mai ◽  
Qunzhang Zhan ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Macrophage Polarization ◽  
Density Lipoprotein ◽  
Lipid Lowering ◽  
Foam Cell Formation ◽  
M2 Macrophage ◽  
Mrna Levels ◽  
High Fat ◽  
Plaque Stability ◽  
M1 Macrophage

Macrophage polarization plays a vital impact in triggering atherosclerosis (AS) progression and regression. Huang-Lian-Jie-Du Decoction (HLJDD), a famous traditional Chinese decoction, displays notable anti-inflammatory and lipid-lowering effects in different animal models. However, its effects and mechanisms on AS have not been clearly defined. We determined whether HLJDD attenuated atherosclerosis and plaques vulnerability by regulating macrophage polarization in ApoE−/− mice induced by high-fat diet (HFD). Furthermore, we investigated the effects of HLJDD on macrophage polarization in oxidized low-density lipoprotein (ox-LDL) induced RAW264.7 cells. For in vivo assay, compared with the model group, HLJDD ameliorated lipid metabolism, with significantly decreased levels of serum triglyceride, total cholesterol (CHOL), and lipid density lipoprotein. HLJDD suppressed serum tumor necrosis factor α (TNF-α) and IL-1β levels with increased serum IL-10 level, and inhibited mRNA level of NLRP3 inflammasome in carotid tissues. HLJDD enhanced carotid lesion stability by decreasing macrophage infiltration together with increased expression of collagen fibers and α-SMA. Moreover, HLJDD inhibited M1 macrophage polarization, which decreased the expression and mRNA levels of M1 markers [inducible nitric oxide synthase (iNOS) and CD86]. HLJDD enhanced alternatively activated macrophage (M2) activation, which increased the expression and mRNA levels of M2 markers (Arg-1 and CD163). For in vitro assay, HLJDD inhibited foam cell formation in RAW264.7 macrophages disturbed by ox-LDL. Besides, groups with ox-LDL plus HLJDD drug had a lower expression of CD86 and mRNA levels of iNOS, CD86, and IL-1β, but higher expression of CD163 and mRNA levels of Arg-1, CD163, and IL-10 than ox-LDL group. Collectively, our results revealed that HLJDD alleviated atherosclerosis and promoted plaque stability by suppressing M1 polarization and enhancing M2 polarization.

Role of 14-3-3η protein on cardiac fatty acid metabolism and macrophage polarization after high fat diet induced type 2 diabetes mellitus

2017 ◽  
Vol 88 ◽  
pp. 92-99 ◽  
Author(s):  
Remya Sreedhar ◽  
Somasundaram Arumugam ◽  
Rajarajan A. Thandavarayan ◽  
Vengadeshprabhu Karuppagounder ◽  
Yusuke Koga ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Fatty Acid ◽  
High Fat Diet ◽  
Acid Metabolism ◽  
Macrophage Polarization ◽  
High Fat

scholarly journals Metabolomic and genomic profiling of n‐3 polyunsaturated fatty acid effects on muscle metabolism in mice fed a high fat diet

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Arwa Aljawadi ◽  
Monique LeMieux ◽  
Nishan S Kalupahana ◽  
Nalin Siriwardhana ◽  
Kate Claycombe ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Polyunsaturated Fatty Acid ◽  
High Fat Diet ◽  
Muscle Metabolism ◽  
Genomic Profiling ◽  
High Fat
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