scholarly journals Long-term exposure to dietary sources of genistein induces estrogen-independence in the human breast cancer (MCF-7) xenograft model

2014 ◽  
Vol 59 (3) ◽  
pp. 413-423 ◽  
Author(s):  
Juan E. Andrade ◽  
Young H. Ju ◽  
Chandra Baker ◽  
Daniel R. Doerge ◽  
William G. Helferich
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Xenograft Model ◽  
Human Breast ◽  
Mcf 7

Crosstalk with insulin and dependence on PI3K/Akt/mTOR rather than MAPK pathways in upregulation of basal growth following long-term oestrogen deprivation in three human breast cancer cell lines

2011 ◽  
Vol 5 (2) ◽  
Author(s):  
Swagat Ray ◽  
Philippa D. Darbre
Keyword(s):  
Breast Cancer ◽  
Growth Inhibition ◽  
Cell Lines ◽  
Human Breast Cancer ◽  
Human Breast Cancer Cell ◽  
Breast Cancer Cell Lines ◽  
Human Breast ◽  
Oestrogen Deprivation ◽  
Mcf 7

Abstract: MCF-7, T-47-D, ZR-75-1 human breast cancer cell lines are dependent on oestrogen for growth but can adapt to grow during long-term oestrogen deprivation. This serves as a model for identification of therapeutic targets in endocrine-resistant breast cancer.: An overlooked complication of this model is that it involves more than non-addition of oestrogen, and inadequate attention has been given to separating molecular events associated with each of the culture manipulations.: Insulin and oestradiol were shown to protect MCF-7 cells against upregulation of basal growth, demonstrating a crosstalk in the growth adaptation process. Increased phosphorylation of p44/42MAPK and c-Raf reflected removal of insulin from the medium and proliferation of all three cell lines was inhibited to a lesser extent by PD98059 and U0126 following long-term oestrogen/insulin withdrawal, demonstrating a reduced dependence on the MAPK pathway. By contrast, long-term oestrogen/insulin deprivation did not alter levels of phosphorylated Akt and did not alter the dose-response of growth inhibition with LY294002 in any of the three cell lines. The IGF1R inhibitor picropodophyllin inhibited growth of all MCF-7 cells but only in the long-term oestrogen/insulin-deprived cells was this paralleled by reduction in phosphorylated p70S6K, a downstream target of mTOR. Long-term oestrogen/insulin-deprived MCF-7 cells had higher levels of phosphorylated p70S6K and developed increased sensitivity to growth inhibition by rapamycin.: The greater sensitivity to growth inhibition by rapamycin in all three cell lines following long-term oestrogen/insulin deprivation suggests rapamycin-based therapies might be more effective in breast cancers with acquired oestrogen resistance.

Long-term inhibitory effects of a novel anti-estrogen on the growth of ZR-75-1 and MCF-7 human breast cancer tumors in nude mice

1997 ◽  
Vol 73 (5) ◽  
pp. 735-739 ◽  
Author(s):  
Shouqi Luo ◽  
Céline Martel ◽  
Sylvain Gauthier ◽  
Yves Mérand ◽  
Alain Bélanger ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Nude Mice ◽  
Human Breast Cancer ◽  
Human Breast ◽  
Inhibitory Effects ◽  
Mcf 7
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