Grape seed proanthocyanidins repress the hepatic lipid regulators miR-33 and miR-122 in rats

2012 ◽  
Vol 56 (11) ◽  
pp. 1636-1646 ◽  
Author(s):  
Laura Baselga-Escudero ◽  
Cinta Bladé ◽  
Aleix Ribas-Latre ◽  
Ester Casanova ◽  
M. Josepa Salvadó ◽  
...  
Keyword(s):  
Grape Seed ◽  
Hepatic Lipid ◽  
Grape Seed Proanthocyanidins ◽  
Lipid Regulators

Grape Seed Proanthocyanidins Protect N2a Cells against Ischemic Injury via Endoplasmic Reticulum Stress and Mitochondrial-associated Pathways

2019 ◽  
Vol 18 (4) ◽  
pp. 334-341 ◽  
Author(s):  
Kun Fu ◽  
Liqiang Chen ◽  
Lifeng Miao ◽  
Yan Guo ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Membrane Potential ◽  
Cell Viability ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Grape Seed ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Grape Seed Proanthocyanidins ◽  
N2a Cells ◽  
Caspase 12

Background/Objective: Grape seed proanthocyanidins (GSPs) are a group of polyphenolic bioflavonoids, which possess a variety of biological functions and pharmacological properties. We studied the neuroprotective effects of GSP against oxygen-glucose deprivation/reoxygenation (OGD/R) injury and the potential mechanisms in mouse neuroblastoma N2a cells. Methods: OGD/R was conducted in N2a cells. Cell viability was evaluated by CCK-8 and LDH release assay. Apoptosis was assessed by TUNEL staining and flow cytometry. Protein levels of cleaved caspase-3, Bax and Bcl-2 were detected by Western blotting. CHOP, GRP78 and caspase-12 mRNA levels were assessed by real-time PCR. JC-1 dying was used to detect mitochondrial membrane potential. ROS levels, activities of endogenous antioxidant enzymes and ATP production were examined to evaluate mitochondrial function. Results: GSP increased cell viability after OGD/R injury in a dose-dependent manner. Furthermore, GSP inhibited cell apoptosis, reduced the mRNA levels of CHOP, GRP78 and caspase-12 (ER stressassociated genes), restored mitochondrial membrane potential and ATP generation, improved activities of endogenous anti-oxidant ability (T-AOC, GXH-Px, and SOD), and decreased ROS level. Conclusion: Our findings suggest that GSP can protect N2a cells from OGD/R insult. The mechanism of anti-apoptotic effects of GSP may involve attenuating ER stress and mitochondrial dysfunction.

Physicochemical properties and in vitro digestion of extruded rice with grape seed proanthocyanidins

2020 ◽  
Vol 95 ◽  
pp. 103064 ◽  
Author(s):  
Yuxue Zheng ◽  
Jinhu Tian ◽  
Yukiharu Ogawa ◽  
Xiangli Kong ◽  
Shiguo Chen ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
In Vitro Digestion ◽  
Grape Seed ◽  
Grape Seed Proanthocyanidins

Utilization of plant-based protein-polyphenol complexes to form and stabilize emulsions: Pea proteins and grape seed proanthocyanidins

2020 ◽  
Vol 329 ◽  
pp. 127219
Author(s):  
Taotao Dai ◽  
Ti Li ◽  
Ruyi Li ◽  
Hualu Zhou ◽  
Chengmei Liu ◽  
...  
Keyword(s):  
Grape Seed ◽  
Grape Seed Proanthocyanidins ◽  
Pea Proteins
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