[6]-Gingerol induces bone loss in ovary intact adult mice and augments osteoclast function via the transient receptor potential vanilloid 1 channel

2012 ◽  
Vol 56 (12) ◽  
pp. 1860-1873 ◽  
Author(s):  
Kainat Khan ◽  
Akanksha Singh ◽  
Monika Mittal ◽  
Kunal Sharan ◽  
Nidhi Singh ◽  
...  
Keyword(s):  
Bone Loss ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Transient Receptor Potential Vanilloid ◽  
Adult Mice ◽  
Osteoclast Function ◽  
Transient Receptor

scholarly journals Interaction between TRPV1-expressing neurons in the hypothalamus

2019 ◽  
Vol 121 (1) ◽  
pp. 140-151 ◽  
Author(s):  
Adrien J. R. Molinas ◽  
Lucie D. Desmoulins ◽  
Brooke V. Hamling ◽  
Sierra M. Butcher ◽  
Imran J. Anwar ◽  
...  
Keyword(s):  
Transient Receptor Potential ◽  
Direct Interaction ◽  
Receptor Potential ◽  
Functional Expression ◽  
Hypothalamic Nucleus ◽  
Transient Receptor Potential Vanilloid ◽  
Adult Mice ◽  
Hypothalamic Nuclei ◽  
Transient Receptor

Transient receptor potential vanilloid type 1 (TRPV1) is a ligand-gated ion channel expressed in the peripheral and central nervous systems. TRPV1-dependent mechanisms take part in a wide range of physiological and pathophysiological pathways including the regulation of homeostatic functions. TRPV1 expression in the hypothalamus has been described as well as evidence that TRPV1-dependent excitatory inputs to hypothalamic preautonomic neurons are diminished in diabetic conditions. Here we aimed to determine the functional expression of TRPV1 in two hypothalamic nuclei known to be involved in the central control of metabolism and to test the hypothesis that TRPV1-expressing neurons receive TRPV1-expressing inputs. A mouse model (TRPV1Cre/tdTom) was generated to identify TRPV1-expressing cells and determine the cellular properties of TRPV1-expressing neurons in adult mice. Our study demonstrated the functional expression of TRPV1 in the dorsomedial hypothalamic nucleus and paraventricular nucleus in adult mice. Our findings revealed that a subset of TRPV1Cre/tdTom neurons receive TRPV1-expressing excitatory inputs, indicating direct interaction between TRPV1-expressing neurons. In addition, astrocytes likely play a role in the modulation of TRPV1-expressing neurons. In summary, this study identified specific hypothalamic regions where TRPV1 is expressed and functional in adult mice and the existence of direct connections between TRPV1Cre/tdTom neurons. NEW & NOTEWORTHY Transient receptor potential vanilloid type 1 (TRPV1) is expressed in the hypothalamus, and TRPV1-dependent regulation of preautonomic neurons is decreased in hyperglycemic conditions. Our study demonstrated functional expression of TRPV1 in two hypothalamic nuclei involved in the control of energy homeostasis. Our results also revealed that a subset of TRPV1-expressing neurons receive TRPV1-expressing excitatory inputs. These findings suggest direct interaction between TRPV1-expressing neurons.

scholarly journals Ca2+ Signalling Induced by NGF Identifies a Subset of Capsaicin-Excitable Neurons Displaying Enhanced Chemo-Nociception in Dorsal Root Ganglion Explants from Adult pirt-GCaMP3 Mouse

2021 ◽  
Vol 22 (5) ◽  
pp. 2589
Author(s):  
Gary W. Lawrence ◽  
Tomas H. Zurawski ◽  
J. Oliver Dolly
Keyword(s):  
Dorsal Root ◽  
Transient Receptor Potential ◽  
Thermal Hyperalgesia ◽  
Receptor Potential ◽  
Transient Receptor Potential Vanilloid ◽  
Cellular Mechanisms ◽  
Fluorescent Reporter ◽  
Neuronal Populations ◽  
Adult Mice ◽  
Transient Receptor

Nociceptors sense hazards via plasmalemmal cation channels, including transient receptor potential vanilloid 1 (TRPV1). Nerve growth factor (NGF) sensitises TRPV1 to capsaicin (CAPS), modulates nociceptor excitability and induces thermal hyperalgesia, but cellular mechanisms remain unclear. Confocal microscopy was used to image changes in intracellular Ca2+ concentration ([Ca2+]i) across neuronal populations in dorsal root ganglia (DRG) explants from pirt-GCaMP3 adult mice, which express a fluorescent reporter in their sensory neurons. Raised [Ca2+]i was detected in 84 neurons of three DRG explants exposed to NGF (100 ng/mL) and most (96%) of these were also excited by 1 μM CAPS. NGF elevated [Ca2+]i in about one-third of the neurons stimulated by 1 μM CAPS, whether applied before or after the latter. In neurons excitable by NGF, CAPS-evoked [Ca2+]i signals appeared significantly sooner (e.g., respective lags of 1.0 ± 0.1 and 1.9 ± 0.1 min), were much (>30%) brighter and lasted longer (6.6 ± 0.4 vs. 3.9 ± 0.2 min) relative to those non-responsive to the neurotrophin. CAPS tachyphylaxis lowered signal intensity by ~60% but was largely prevented by NGF. Increasing CAPS from 1 to 10 μM nearly doubled the number of cells activated but only modestly increased the amount co-activated by NGF. In conclusion, a sub-population of the CAPS-sensitive neurons in adult mouse DRG that can be excited by NGF is more sensitive to CAPS, responds with stronger signals and is further sensitised by transient exposure to the neurotrophin.

Transient receptor potential vanilloid 4 deficiency suppresses unloading-induced bone loss

2008 ◽  
Vol 216 (1) ◽  
pp. 47-53 ◽  
Author(s):  
Fumitaka Mizoguchi ◽  
Atsuko Mizuno ◽  
Tadayoshi Hayata ◽  
Kazuhisa Nakashima ◽  
Stefan Heller ◽  
...  
Keyword(s):  
Bone Loss ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Transient Receptor Potential Vanilloid ◽  
Transient Receptor
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