Geraniin-mediated apoptosis by cleavage of focal adhesion kinase through up-regulation of Fas ligand expression in human melanoma cells

2008 ◽  
Vol 52 (6) ◽  
pp. 655-663 ◽  
Author(s):  
Jang-Chang Lee ◽  
Chih-Yen Tsai ◽  
Jung-Yie Kao ◽  
Ming-Ching Kao ◽  
Shih-Chang Tsai ◽  
...  
Keyword(s):  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Fas Ligand ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Human Melanoma Cells ◽  
Ligand Expression

scholarly journals Triptolide-Mediated Apoptosis by Suppression of Focal Adhesion Kinase through Extrinsic and Intrinsic Pathways in Human Melanoma Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-10
Author(s):  
Haw-Young Kwon ◽  
Kyoung-Sook Kim ◽  
Ji-Sue Baik ◽  
Hyung-In Moon ◽  
Ji-Won Lee ◽  
...  
Keyword(s):  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Caspase 3 ◽  
Human Cancer ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Caspase 8 ◽  
Cytochrome C Release ◽  
Human Melanoma Cells ◽  
Induced Apoptosis

Triptolide (TPL) has been shown to inhibit cell proliferation and induce apoptosis in various human cancer cells; however, the precise mechanism of apoptosis induced by TPL in human melanoma cells has not yet been elucidated. In this study, we investigated the precise mechanism underlying cytocidal effects of TPL on human melanoma cells. Treatment of human melanoma cells with TPL significantly inhibited cell growth and induced apoptosis, as evidenced by flow cytometry and annexin V-fluorescein isothiocyanate analyses. TPL increased the levels of Fas and Fas-associated death domain (FADD) and induced cleavage of Bid by activation of caspase-8 and cytochrome c release from mitochondria to the cytosol, which resulted in activation of caspase-9 and caspase-3. Moreover, TPL-induced apoptosis in SK-MEL-2 cells was mediated through dephosphorylation of focal adhesion kinase (FAK) and its cleavage by caspase-8-mediated caspase-3 activation via upregulation of Fas expression. We also found that TPL mediated the dissociation of receptor-interacting protein (RIP) from FAK and enhanced the formation of RIP/Fas complex formation initiating cell death. In conclusion, our data firstly demonstrated that TPL induces apoptosis by both extrinsic and intrinsic apoptosis pathways in human melanoma cells and identified that RIP shuttles between Fas and FAK to mediate apoptosis.

scholarly journals The origin of the expressed retrotransposed gene ACTBL2 and its influence on human melanoma cells’ motility and focal adhesion formation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Natalia Malek ◽  
Aleksandra Michrowska ◽  
Ewa Mazurkiewicz ◽  
Ewa Mrówczyńska ◽  
Paweł Mackiewicz ◽  
...  
Keyword(s):  
Focal Adhesion ◽  
Actin Polymerization ◽  
Adhesion Formation ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Small Subset ◽  
Human Melanoma Cells ◽  
Focal Adhesion Formation ◽  
Actin Isoform ◽  
Melanoma Cell Lines

AbstractWe have recently found that β-actin-like protein 2 (actbl2) forms complexes with gelsolin in human melanoma cells and can polymerize. Phylogenetic and bioinformatic analyses showed that actbl2 has a common origin with two non-muscle actins, which share a separate history from the muscle actins. The actin groups’ divergence started at the beginning of vertebrate evolution, and actbl2 actins are characterized by the largest number of non-conserved amino acid substitutions of all actins. We also discovered that ACTBL2 is expressed at a very low level in several melanoma cell lines, but a small subset of cells exhibited a high ACTBL2 expression. We found that clones with knocked-out ACTBL2 (CR-ACTBL2) or overexpressing actbl2 (OE-ACTBL2) differ from control cells in the invasion, focal adhesion formation, and actin polymerization ratio, as well as in the formation of lamellipodia and stress fibers. Thus, we postulate that actbl2 is the seventh actin isoform and is essential for cell motility.

Adipocytes Contribute to Resistance of Human Melanoma Cells to Chemotherapy and Targeted Therapy

2014 ◽  
Vol 21 (10) ◽  
pp. 1255-1267 ◽  
Author(s):  
M. Chi ◽  
J. Chen ◽  
Y. Ye ◽  
Hsin-Yi Tseng ◽  
F. Lai ◽  
...  
Keyword(s):  
Targeted Therapy ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Human Melanoma Cells
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