Knockouts of Se-glutathione peroxidase-1 and Cu,Zn superoxide dismutase exert different impacts on femoral mechanical performance of growing mice

2008 ◽  
Vol 52 (11) ◽  
pp. 1334-1339 ◽  
Author(s):  
Xiaodan Wang ◽  
Elizabeth A. Gillen ◽  
Marjolein C. H. van der Meulen ◽  
Xin Gen Lei
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Mechanical Performance ◽  
Glutathione Peroxidase 1

Effects of transgene expression of superoxide dismutase and glutathione peroxidase on pulmonary epithelial cell growth in hyperoxia

2005 ◽  
Vol 288 (4) ◽  
pp. L718-L726 ◽  
Author(s):  
Hshi-chi Koo ◽  
Jonathan M. Davis ◽  
Yuchi Li ◽  
Dimitrios Hatzis ◽  
Harry Opsimos ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Epithelial Cells ◽  
Lung Injury ◽  
Glutathione Peroxidase ◽  
A549 Cells ◽  
Alveolar Epithelial Cells ◽  
Glutathione Peroxidase 1 ◽  
Mitochondrial Oxidation ◽  
Optimal Protection ◽  
In Cells

Prolonged exposure to supraphysiological oxygen concentrations results in the generation of reactive oxygen species, which can cause significant lung injury in critically ill patients. Supplementation with human recombinant antioxidant enzymes (AOE) may mitigate hyperoxic lung injury, but it is unclear which combination and concentration will optimally protect pulmonary epithelial cells. First, stable cell lines were generated in alveolar epithelial cells (MLE12) overexpressing one or more of the following AOE: Mn superoxide dismutase (MnSOD), CuZnSOD, or glutathione peroxidase 1. Next, A549 cells were transduced with 50–300 particles/cell of recombinant adenovirus containing either LacZ or each of the three AOE (alone or in combination). Cells were then exposed to 95% O2 for up to 3 days, with cell number and viability determined daily. Overexpression of either MnSOD (primarily mitochondrial) or CuZnSOD (primarily cytosolic) reversed the growth inhibitory effects of hyperoxia within the first 48 h of exposure, resulting in a significant increase in viable cells ( P < 0.05), with 1.5- to 3-fold increases in activity providing optimal protection. Protection from mitochondrial oxidation was confirmed by assessing aconitase activity, which was significantly improved in cells overexpressing MnSOD ( P < 0.05). Data indicate that optimal protection from hyperoxic injury occurs in cells coexpressing MnSOD and glutathione peroxidase 1, with prevention of mitochondrial oxidation being a critical factor. This has important implications for clinical trials in preterm infants receiving SOD supplementation to prevent acute and chronic lung injury.

Analysis of Manganese Superoxide Dismutase and Glutathione Peroxidase 1 Gene Polymorphisms in Vitiligo

2016 ◽  
Vol 54 (4) ◽  
pp. 438-447
Author(s):  
Havva Yıldız Seçkin ◽  
Göknur Kalkan ◽  
İlknur Bütün ◽  
Ali Akbaş ◽  
Yalçın Baş ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Gene Polymorphisms ◽  
Manganese Superoxide Dismutase ◽  
Glutathione Peroxidase 1 ◽  
Manganese Superoxide

Genotype???activity relationship for Mn-superoxide dismutase, glutathione peroxidase 1 and catalase in humans

2006 ◽  
Vol 16 (4) ◽  
pp. 279-286 ◽  
Author(s):  
Maria Bastaki ◽  
Karen Huen ◽  
Paolo Manzanillo ◽  
Neha Chande ◽  
Connie Chen ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Activity Relationship ◽  
Glutathione Peroxidase 1

Distribution of superoxide dismutase 1 and glutathione peroxidase 1 in the cyclic canine endometrium

2016 ◽  
Vol 86 (3) ◽  
pp. 738-748 ◽  
Author(s):  
Celso Santos ◽  
Maria dos Anjos Pires ◽  
Dario Santos ◽  
Rita Payan-Carreira
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Superoxide Dismutase 1 ◽  
Glutathione Peroxidase 1
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