Isometric tetanic force measurement method of the tibialis anterior in the rat

Microsurgery ◽  
2008 ◽  
Vol 28 (6) ◽  
pp. 452-457 ◽  
Author(s):  
Richard H. Shin ◽  
Torpon Vathana ◽  
Goetz A. Giessler ◽  
Patricia F. Friedrich ◽  
Allen T. Bishop ◽  
...  
Keyword(s):  
Tibialis Anterior ◽  
Measurement Method ◽  
Force Measurement ◽  
Tetanic Force

An experimental study to determine and correlate choline acetyltransferase assay with functional muscle testing after nerve injury

2014 ◽  
Vol 120 (5) ◽  
pp. 1125-1130 ◽  
Author(s):  
Torpon Vathana ◽  
Tim H. J. Nijhuis ◽  
Patricia F. Friedrich ◽  
Allen T. Bishop ◽  
Alexander Y. Shin
Keyword(s):  
Tibialis Anterior ◽  
Peroneal Nerve ◽  
Force Measurement ◽  
Tibialis Anterior Muscle ◽  
Muscle Weight ◽  
Tetanic Force ◽  
Chat Activity ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2

Object Choline acetyltransferase (ChAT) is an enzyme synthesized within the body of a motor neuron whose role is to form the neurotransmitter acetylcholine. Quantification of ChAT levels in motor or mixed nerves has been proposed to provide information regarding the viability of a proximal nerve stump for motor neurotization following brachial plexus injury. To do so requires information regarding normal ChAT levels and those in injured nerves, as well as the correlation of ChAT level determined at surgery with eventual motor recovery. The purpose of this study was to determine ChAT activity in the normal and injured sciatic/peroneal nerve in a rat model, evaluate the correlation between ChAT and motor recovery, find the relationship between ChAT activity and isometric muscle force, and elucidate the parallel between ChAT activity and acetylcholinesterase (AChE) activity. Methods Sixty animals were divided into 3 groups. The sciatic nerves in Group 1 were transected without repair. Nerves in Group 2 were transected and repaired. Nerves in Group 3 sustained a crush injury followed by transection and reconstruction. All animals were allowed 12 weeks of recovery followed by evaluation of ChAT levels in the peroneal nerve, correlated with measures of maximal isometric tibialis anterior muscle force and muscle weight (the operated side normalized to the control side). Karnovsky AChE staining of peroneal nerve segments was also compared with radiochemical assay of ChAT activity in the same nerve. Results A significant difference in the tibialis anterior isometric tetanic force and the tibialis anterior muscle weight index (TAMI) was noted between Group 1 and Groups 2 and 3 (p < 0.0001); no significant difference was found comparing Group 2 with Group 3. The correlation between the force measurement and the TAMI was 0.382. Both AChE measurement and ChAT activity demonstrated significantly fewer fibers in the operated nerve compared with the contralateral nerve. Intergroup variability could also be illustrated using these tests. The correlation coefficient between the isometric tetanic force measurement and the ChAT analysis in Groups 1 and 2 was 0.468. The correlation for the AChE staining and the isometric tetanic force measurement was 0.111. The correlation between the TAMI and the ChAT levels was 0.773. The correlation between the TAMI and the AChE-stained fibers was 0.640. Correlating AChE staining to the ChAT analysis produced a correlation of 0.712. Conclusions The great variability in all groups and weak correlations to the functional muscle assessments and the ChAT radiochemical assay made this technique an unreliable method of determining motor nerve viability.

Maximum Isometric Tetanic Force Measurement of the Tibialis Anterior Muscle in the Rat

10.3791/61926 ◽  
2021 ◽  
Author(s):  
Meiwand Bedar ◽  
Tiam M. Saffari ◽  
Patricia F. Friedrich ◽  
Guilherme Giusti ◽  
Allen T. Bishop ◽  
...  
Keyword(s):  
Tibialis Anterior ◽  
Force Measurement ◽  
Tibialis Anterior Muscle ◽  
Tetanic Force

Ultrasonic measurement of clamping force for injection molding machine

2019 ◽  
Vol 39 (4) ◽  
pp. 388-396 ◽  
Author(s):  
Peng Zhao ◽  
Yao Zhao ◽  
Jianfeng Zhang ◽  
Junye Huang ◽  
Neng Xia ◽  
...  
Keyword(s):  
Injection Molding ◽  
Measurement Method ◽  
Large Scale ◽  
Force Measurement ◽  
Low Cost ◽  
Ultrasonic Method ◽  
Injection Molding Process ◽  
Propagation Time ◽  
Clamping Force ◽  
Molding Process

AbstractAn online and feasible clamping force measurement method is important in the injection molding process and equipment. Based on the sono-elasticity theory, anin situclamping force measurement method using ultrasonic technology is proposed in this paper. A mathematical model is established to describe the relationship between the ultrasonic propagation time, mold thickness, and clamping force. A series of experiments are performed to verify the proposed method. Experimental findings show that the measurement results of the proposed method agree well with those of the magnetic enclosed-type clamping force tester method, with difference squares less than 2 (MPa)2and errors bars less than 0.7 MPa. The ultrasonic method can be applied in molds of different thickness, injection molding machines of different clamping scales, and large-scale injection cycles. The proposed method offers advantages of being highly accurate, highly stable, simple, feasible, non-destructive, and low-cost, providing significant application prospects in the injection molding industry.

Feasibility Study for Vision-Based Cable Force Measurement Using Smartphone

2016 ◽  
Author(s):  
Xuefeng Zhao ◽  
Kwang Ri ◽  
Yan Yu ◽  
Chunil Kang ◽  
Mingchu Li ◽  
...  
Keyword(s):  
Measurement Method ◽  
Force Measurement ◽  
Smartphone Application ◽  
Cable Model ◽  
Construction Period ◽  
Service Period ◽  
Cable Force ◽  
Vibration Responses ◽  
Cable Force Measurement ◽  
Circular Target

An accurate cable force measurement is one of very important practical problems during construction period as well as during service period of cable stayed bridge. In the recent years, with the advances in smartphone technologies, it is possible to rapidly evaluate structural health status and postevent damage using ubiquitous smartphones. In this paper, a novel vision-based cable force measurement method using smartphone camera is proposed for the first time, which enable to estimate cable force by recognizing cable vibration using smartphone camera, and then cable model test is carried out to verify the feasibility of the proposed method. The comparison test between the smartphone application Orion-CC measuring cable force from smartphone built-in accelerometer and the proposed method is conducted on a laboratory scale cable model with different sampling rates. In the proposed method, the vibration responses of cable are obtained by monitoring displacements of a preprinted black circular target attached on the cable model using smartphone camera. The test results showed satisfactory agreements between two methods in both frequency domain and cable force value, demonstrating the feasibility of the proposed cable force measurement method and its advantages such as convenience, ease of operation, and speediness.

Average but not continuous speed match between motoneurons and muscle units of rat tibialis anterior

1993 ◽  
Vol 70 (4) ◽  
pp. 1300-1306 ◽  
Author(s):  
R. Bakels ◽  
D. Kernell
Keyword(s):  
Tibialis Anterior ◽  
Time Course ◽  
Muscle Fibers ◽  
Total Duration ◽  
Medial Gastrocnemius ◽  
Tetanic Force ◽  
Time To Peak ◽  
Time Courses ◽  
Isometric Twitch ◽  
Fatigue Sensitivity

1. Properties of single motoneuron/muscle-unit combinations were determined for tibialis anterior (TA) in rats anesthetized with pentobarbital. The TA observations were systematically compared with those obtained earlier by the use of the same techniques from rat medial gastrocnemius (MG). 2. TA motoneurons were investigated with regard to afterhyperpolarization (AHP; total duration 32-74 ms, amplitude 0.39-4.96 mV) and axonal conduction velocity (41-79 m/s). TA muscle-unit measurements included the time course of the isometric twitch (time-to-peak force 10.8-18.0 ms; total duration 42-92 ms), the maximum tetanic force (22-217 mN), and a measure of fatigue sensitivity (fatigue index 5-100%). The range of twitch and AHP durations ("speed range") was markedly smaller in the present TA material than for MG. 3. The mean duration of the TA motoneuronal AHP (49 +/- 8 ms, mean +/- SD) was close to that of its muscle-unit twitch (56 +/- 12 ms). Thus an "average" speed match existed between TA motoneurons and their muscle fibers. 4. For TA there was no correlation between the time courses of AHP and twitch. Thus there was for TA no "continuous" speed match between the motoneurons and their muscle fibers. 5. For TA twitches or AHPs studied separately, there was a significant correlation between different time course measures. Furthermore, compared with TA units having relatively fast twitches, those with slower twitches tended to show 1) a smaller maximum tetanic force and 2) a greater AHP amplitude. Fatigue-resistant units tended to have slower twitches than fatigue-sensitive ones.(ABSTRACT TRUNCATED AT 250 WORDS)

Vision-based force measurement using geometric moment invariants

2012 ◽  
Vol 226 (10) ◽  
pp. 2589-2601
Author(s):  
Wen Hu ◽  
Shigang Wang ◽  
Chun Hu ◽  
Hongtao Liu ◽  
Jinqiu Mo
Keyword(s):  
Measurement Method ◽  
Feature Vector ◽  
Force Measurement ◽  
Shape Descriptor ◽  
Support Vector ◽  
Moment Invariants ◽  
Geometric Moment ◽  
New Vision ◽  
Multi Class Classification ◽  
Standard Library

This article presents a new vision-based force measurement method to measure microassembly forces without directly computing the deformation. The shape descriptor of geometric moment invariants is used as a feature vector to describe the implicit relationship between an applied force and the deformation. Then, a standard library is established to map the corresponding relationship between the deformed cantilever under known forces and a set of feature vectors. Finally, a support vector machine compares the feature vector of deformed cantilever under an unknown force with those in the standard library, implements multi-class classification and predicts the unknown force. The vision-based force measurement method is validated for eight simulated microcantilevers of different sizes. Both regional and boundary moment invariants are used to constitute the feature vector. Simulated results show that the force measurement precision varies with length, width and height of cantilevers. If length increases and width and height decrease, the precision is higher. This trend can provide a reference for mechanism design of microcantilevers and microgrippers.

Application of Coercimeters for Equipment Diagnostics

NDT World ◽  
2015 ◽  
Vol 18 (4) ◽  
pp. 22-24
Author(s):  
Сивирюк ◽  
Vladimir Siviryuk ◽  
Безруков ◽  
Aleksey Bezrukov
Keyword(s):  
Coercive Force ◽  
Measurement Method ◽  
Residual Life ◽  
Force Measurement ◽  
Early Recognition ◽  
Metal Structure ◽  
Pressure Vessels ◽  
Metal Fatigue ◽  
Emergency Situations ◽  
Manual Testing

Introduction. Changes of metal structure commonly result in equipment failures; early recognition of such changes is necessary in order to prevent emergency situations. Coercimetry is a simple and informative method for metal structure estimation. Method. The coercive force measurement method was used for estimation of the metal fatigue state. Results. The article presents the results of coercive force measurement application for the diagnosis of blast furnace jackets, overhead cranes, load-bearing elements of buildings and structures, pressure vessels, industrial pipelines, chimney stacks, ropes of hoisting devices. Manual testing of the equipment working under metallurgical production conditions is complicated; in hard-to-reach places and gas hazardous areas the stationary testing system has been used; the system has included permanently installed sensors connected to the switchboard. The equipment residual life has been determined by the coercive force maximum values with the use of nomograms. Conclusions. The coercive force measurement method for diagnostic survey of the equipment working under considerable thermal effect makes it possible to determine the metal stress state, predict zones of corrosion cracks formation and development, estimate the residual operation life, plan worn areas replacement/repair and as the final result – reduce unplanned and emergency shutdowns.

Precise force measurement method by a Y-shaped cavity dual-frequency laser

2011 ◽  
Vol 9 (10) ◽  
pp. 101201-101204 ◽  
Author(s):  
张斌 Guangzong Xiao ◽  
肖光宗 Xingwu Long ◽  
龙兴武 Bin Zhang ◽  
李耿 Geng Li
Keyword(s):  
Measurement Method ◽  
Force Measurement ◽  
Dual Frequency ◽  
Frequency Laser

Innervation ratio is an important determinant of force in normal and reinnervated rat tibialis anterior muscles

1992 ◽  
Vol 67 (5) ◽  
pp. 1385-1403 ◽  
Author(s):  
J. E. Totosy de Zepetnek ◽  
H. V. Zung ◽  
S. Erdebil ◽  
T. Gordon
Keyword(s):  
Muscle Fiber ◽  
Tibialis Anterior ◽  
Periodic Acid ◽  
Muscle Fibers ◽  
Fiber Types ◽  
Normal Muscle ◽  
Tetanic Force ◽  
Fiber Area ◽  
Single Motor Units ◽  
Force Range

1. The technique of glycogen depletion and periodic acid-Schiff (PAS) staining, which identifies glycogen-free muscle fibers, was used to directly count the number (N) and measure the cross-sectional area (CSA) of muscle fibers in single motor units (MUs) from normal and reinnervated tibialis anterior (TA) muscles. Indirect estimates, derived from the proportions of muscle fiber types to MU types, were also made, and force per unit area (or specific force, SF) was calculated. Previous results using direct and indirect approaches have been contradictory. To shed more light on this issue, the relative contributions of N, mean fiber area (A), and SF to muscle-unit force were determined by the use of both methods. 2. TA muscles were examined in experimental rats 3.5-10 mo after cutting and resuturing the common peroneal nerve in one hindlimb and in muscles in age-matched control rats. Ventral roots were dissected to isolate and characterize single MUs according to contraction speed, sag, and fatigability. One unit per muscle was selected for repetitive tetanic stimulation designed to deplete muscle fiber glycogen stores. Muscles were removed for identification of the unit with the PAS reaction and histochemical fiber typing by the use of modified standard techniques. 3. In the total population of MUs sampled, isometric tetanic force ranged from 5 to 441 mN in normal muscles and from 5 to 498 mN in reinnervated muscles, and the mean values were not significantly different. In the smaller sample of glycogen-depleted units from normal muscle, for a force range of 14-217 mN, N varied from 57 to 202, and A varied from 1,135 +/- 45 to 6,706 +/- 172 (SE) microns2. Within each unit the variation in fiber area is broad. After reinnervation, for a force range of 30-278 mN, N varied from 70 to 374, and A varied from 1,694 +/- 81 to 5,425 +/- 93 microns2. Mean fiber number was 153 +/- 18 in reinnervated muscle, which is significantly higher (P less than 0.01) than the normal value of 121 +/- 9. 4. The contribution of N and A to MU tetanic force was assessed by plotting each factor as a function of force on a log-log scale. N accounts for 39% and A for 49% of the variation in force in normal muscle. The contributions are changed after reinnervation where N, accounting for 65% of force, appears to compensate for the reduced range in A, which accounts for only 19% of the variation in force.(ABSTRACT TRUNCATED AT 400 WORDS)

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