QcrB in Mycobacterium tuberculosis : The new drug target of antitubercular agents

2021 ◽  
Author(s):  
Aparna Bahuguna ◽  
Srishti Rawat ◽  
Diwan S. Rawat
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Drug Target ◽  
Antitubercular Agents ◽  
New Drug

scholarly journals The Mycobacterium tuberculosis MEP (2C-methyl-d-erythritol 4-phosphate) pathway as a new drug target

Tuberculosis ◽  
2009 ◽  
Vol 89 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Hyungjin Eoh ◽  
Patrick J. Brennan ◽  
Dean C. Crick
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Drug Target ◽  
New Drug

scholarly journals 4′-Phosphopantetheinyl Transferase PptT, a New Drug Target Required for Mycobacterium tuberculosis Growth and Persistence In Vivo

2012 ◽  
Vol 8 (12) ◽  
pp. e1003097 ◽  
Author(s):  
Cécile Leblanc ◽  
Thomas Prudhomme ◽  
Guillaume Tabouret ◽  
Aurélie Ray ◽  
Sophie Burbaud ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Drug Target ◽  
Phosphopantetheinyl Transferase ◽  
New Drug

CGMP as a New Drug Target in Atrial Fibrillation

2020 ◽  
Author(s):  
N. I. Bork ◽  
N. Grammatika-Pavlidou ◽  
B. Reiter ◽  
E. Girdauskas ◽  
H. Reichenspurner ◽  
...  
Keyword(s):  
Atrial Fibrillation ◽  
Drug Target ◽  
New Drug

Lanthionine Synthetase Component C-Like Protein 2: A New Drug Target for Inflammatory Diseases and Diabetes

2014 ◽  
Vol 15 (6) ◽  
pp. 565-572 ◽  
Author(s):  
Pinyi Lu ◽  
Raquel Hontecillas ◽  
Casandra Philipson ◽  
Josep Bassaganya-Riera
Keyword(s):  
Drug Target ◽  
Inflammatory Diseases ◽  
New Drug ◽  
Component C

scholarly journals Establishing Virulence Associated Polyphosphate Kinase 2 as a drug target for Mycobacterium tuberculosis

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Mamta Singh ◽  
Prabhakar Tiwari ◽  
Garima Arora ◽  
Sakshi Agarwal ◽  
Saqib Kidwai ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mutant Strain ◽  
Guinea Pigs ◽  
High Throughput Screening ◽  
Drug Target ◽  
Wild Type Strain ◽  
Wild Type ◽  
Inorganic Polyphosphate ◽  
Phosphate Donor ◽  
Microbial Stress

Abstract Inorganic polyphosphate (PolyP) plays an essential role in microbial stress adaptation, virulence and drug tolerance. The genome of Mycobacterium tuberculosis encodes for two polyphosphate kinases (PPK-1, Rv2984 and PPK-2, Rv3232c) and polyphosphatases (ppx-1, Rv0496 and ppx-2, Rv1026) for maintenance of intracellular PolyP levels. Microbial polyphosphate kinases constitute a molecular mechanism, whereby microorganisms utilize PolyP as phosphate donor for synthesis of ATP. In the present study we have constructed ppk-2 mutant strain of M. tuberculosis and demonstrate that PPK-2 enzyme contributes to its ability to cause disease in guinea pigs. We observed that ppk-2 mutant strain infected guinea pigs had significantly reduced bacterial loads and tissue pathology in comparison to wild type infected guinea pigs at later stages of infection. We also report that in comparison to the wild type strain, ppk-2 mutant strain was more tolerant to isoniazid and impaired for survival in THP-1 macrophages. In the present study we have standardized a luciferase based assay system to identify chemical scaffolds that are non-cytotoxic and inhibit M. tuberculosis PPK-2 enzyme. To the best of our knowledge this is the first study demonstrating feasibility of high throughput screening to obtain small molecule PPK-2 inhibitors.

Sign in / Sign up

Export Citation Format

Share Document