Fiber optic-based UV-absorption detector cell for high temperature open tubular column liquid chromatography

1994 ◽  
Vol 6 (4) ◽  
pp. 409-414 ◽  
Author(s):  
Lena M. Svensson ◽  
Karin E. Markides
Keyword(s):  
Column Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Temperature ◽  
Fiber Optic ◽  
Uv Absorption ◽  
Open Tubular Column ◽  
Detector Cell

Separation of cations by open-tubular column liquid chromatography

1989 ◽  
Vol 61 (24) ◽  
pp. 2747-2750 ◽  
Author(s):  
Stephan R. Mueller ◽  
Wilhelm. Simon ◽  
H. Michael. Widmer ◽  
Karl. Grolimund ◽  
Gerhard. Schomburg ◽  
...  
Keyword(s):  
Column Liquid Chromatography ◽  
Liquid Chromatography ◽  
Open Tubular Column

Diode Laser-Based Absorption Detector for Conventional-Size Liquid Chromatography

1996 ◽  
Vol 50 (1) ◽  
pp. 28-34 ◽  
Author(s):  
A. J. G. Mank ◽  
H. de Nijs ◽  
H. Lingeman ◽  
U. A. Th. Brinkman ◽  
N. H. Velthorst ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Diode Laser ◽  
Fiber Optic ◽  
Cell Detection ◽  
Gradient Index ◽  
Signal To Noise ◽  
Excitation Light ◽  
Detector Cell ◽  
Gradient Index Lens ◽  
Better Than

A diode laser-based absorption detector is designed for conventional-size liquid chromatography (LC). To this end, various detection setups and individual components have been evaluated. A ratioing system using a 10-mW 670-nm diode laser allowed the detection of 6 × 10−10 M mitoxantrone [signal-to-noise (S/N) = 3; N = root-mean-square (rms) noise], an anti-tumor drug, in a biological matrix without any sample cleanup. Multipass detection and intensity modulation of the excitation light did not improve the detection limit. A fiber-optic detector cell, utilizing a gradient-index lens on the light-guiding fiber, was a good and robust alternative for the standard absorption detector cell. Detection limits with the use of the diode laser-based detector are 20-fold better than those obtained with a commercial absorption detector for LC.

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