Analysis of v-Ha-ras and v-fos oncogene transduction into a mouse epidermal cell line with “initiated” phenotype in culture but normal skin phenotype in vivo

1995 ◽  
Vol 13 (2) ◽  
pp. 96-103 ◽  
Author(s):  
Masato Ueda ◽  
Hideki Kawamura ◽  
Christian Sutter ◽  
Adam Glick ◽  
Stuart H. Yuspa ◽  
...  
Keyword(s):  
Cell Line ◽  
Epidermal Cell ◽  
Normal Skin ◽  
Mouse Epidermal Cell

scholarly journals Establishment of a murine epidermal cell line suitable for in vitro and in vivo skin modelling

2011 ◽  
Vol 11 (1) ◽  
Author(s):  
Carmen Segrelles ◽  
Almudena Holguín ◽  
Pilar Hernández ◽  
José M Ariza ◽  
Jesús M Paramio ◽  
...  
Keyword(s):  
Cell Line ◽  
Epidermal Cell

Properties of a SENCAR mouse epidermal cell line and effects of oncogene introduction

1990 ◽  
Vol 1 (5) ◽  
pp. 381
Author(s):  
Masato Ueda ◽  
Stuart H. Yuspa ◽  
James E. Strickland
Keyword(s):  
Cell Line ◽  
Epidermal Cell ◽  
Mouse Epidermal Cell

Glycosaminoglycan synthesis by Pam cells (mouse epidermal cell line)

1987 ◽  
Vol 279 (4) ◽  
pp. 276-277 ◽  
Author(s):  
A. Konohana ◽  
S. Tajima ◽  
T. Nishikawa
Keyword(s):  
Cell Line ◽  
Epidermal Cell ◽  
Glycosaminoglycan Synthesis ◽  
Mouse Epidermal Cell

Effects of the phorbolester TPA and of the ionophore A 23187 on phospholipase A2 and C activities in the mouse epidermal cell line HEL-30

1987 ◽  
Vol 113 (4) ◽  
pp. 310-318 ◽  
Author(s):  
G. F�rstenberger ◽  
M. Rogers ◽  
J. Faberman ◽  
M. Ganss ◽  
H. Richter ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Cell Line ◽  
Epidermal Cell ◽  
A 23187 ◽  
Mouse Epidermal Cell

Ultrastructural Study of a differentiating human colon carcinoma cell line

1990 ◽  
Vol 48 (3) ◽  
pp. 208-209
Author(s):  
Sylvie Polak-Charcon ◽  
Mehrdad Hekmati ◽  
Yehuda Ben Shaul
Keyword(s):  
Cell Line ◽  
Morphological Changes ◽  
Secretory Granules ◽  
Human Colon ◽  
Cell Types ◽  
Culture Conditions ◽  
Morphological Evidence ◽  
Human Colon Carcinoma Cell ◽  
Colon Cell

The epithelium of normal human colon mucosa “in vivo” exhibits a gradual pattern of differentiation as undifferentiated stem cells from the base of the crypt of “lieberkuhn” rapidly divide, differentiate and migrate toward the free surface. The major differentiated cell type of the intestine observed are: absorptive cells displaying brush border, goblet cells containing mucous granules, Paneth and endocrine cells containing dense secretory granules. These different cell types are also found in the intestine of the 13-14 week old embryo.We present here morphological evidence showing that HT29, an adenocarcinoma of the human colon cell line, can differentiate into various cell types by changing the growth and culture conditions and mimic morphological changes found during development of the intestine in the human embryo.HT29 cells grown in tissue-culture dishes in DMEM and 10% FCS form at late confluence a multilayer of morphologically undifferentiated cell culture covered with irregular microvilli, and devoid of tight junctions (Figs 1-3).

Cytotoxicity of chitosan ultrafine nanoshuttles on MCF-7 cell line as surrogate model for breast cancer

2020 ◽  
Vol 17 ◽  
Author(s):  
Tarek Faris ◽  
Gamaleldin I. Harisa ◽  
Fars K. Alanazi ◽  
Mohamed M. Badran ◽  
Afraa Mohammad Alotaibi ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Factorial Design ◽  
Cell Line ◽  
Cell Lines ◽  
Blood Cells ◽  
Sodium Tripolyphosphate ◽  
Physicochemical Characterization ◽  
Spherical Shape ◽  
Mcf 7

Aim: This study aimed to explore an affordable technique for the fabrication of Chitosan Nanoshuttles (CSNS) at the ultrafine nanoscale less than 100 nm with improved physicochemical properties, and cytotoxicity on the MCF-7 cell line. Background: Despite several studies reported that the antitumor effect of CS and CSNS could achieve intracellular compartment target ability, no enough available about this issue and further studies are required to address this assumption. Objectives: The objective of the current study was to investigate the potential processing variables for the production of ultrafine CSNS (> 100 nm) using Box-Benhken Design factorial design (BBD). This was achieved through a study of the effects of processing factors, such as CS concentration, CS/TPP ratio, and pH of the CS solution, on PS, PDI, and ZP. Moreover, the obtained CSNS was evaluated for physicochemical characteristics, morphology Also, hemocompatibility, and cytotoxicity using Red Blood Cells (RBCs) and MCF-7 cell lines were investigated. Methods: Box-Benhken Design factorial design (BBD) was used in the analysis of different selected variables. The effects of CS concentration, sodium tripolyphosphate (TPP) ratio, and pH on particle size, Polydispersity Index (PDI), and Zeta Potential (ZP) were measured. Subsequently, the prepared CS nanoshuttles were exposed to stability studies, physicochemical characterization, hemocompatibility, and cytotoxicity using red blood cells and MCF-7 cell lines as surrogate models for in vivo study. Result: The present results revealed that the optimized CSNS have ultrafine nanosize, (78.3±0.22 nm), homogenous with PDI (0.131±0.11), and ZP (31.9±0.25 mV). Moreover, CSNS have a spherical shape, amorphous in structure, and physically stable. Also, CSNS has biological safety as indicated by a gentle effect on red blood cell hemolysis, besides, the obtained nanoshuttles decrease MCF-7 viability. Conclusion: The present findings concluded that the developed ultrafine CSNS has unique properties with enhanced cytotoxicity. thus promising for use in intracellular organelles drug delivery.

Sign in / Sign up

Export Citation Format

Share Document