Stepwise transformation of primary thyroid epithelial cells by a mutant Ha-ras oncogene: An in vitro model of tumor progression

1992 ◽  
Vol 6 (2) ◽  
pp. 129-139 ◽  
Author(s):  
Jorge S. Burns ◽  
Jeremy P. Blaydes ◽  
Pat A. Wright ◽  
Louise Lemoine ◽  
Jane A. Bond ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Tumor Progression ◽  
In Vitro Model ◽  
Ras Oncogene ◽  
Vitro Model

Attenuation of catalase activity in the malignant phenotype plays a functional role in an in vitro model for tumor progression

2001 ◽  
Vol 173 (2) ◽  
pp. 115-125 ◽  
Author(s):  
Ashok Gupta ◽  
Brent Butts ◽  
Kevin A. Kwei ◽  
Katerina Dvorakova ◽  
Steven P. Stratton ◽  
...  
Keyword(s):  
Tumor Progression ◽  
Catalase Activity ◽  
Functional Role ◽  
In Vitro Model ◽  
Malignant Phenotype ◽  
Vitro Model

Characterisation of an in vitro system to study maternal communication with spermatozoa

2012 ◽  
Vol 24 (7) ◽  
pp. 988 ◽  
Author(s):  
Ahmed Aldarmahi ◽  
Sarah Elliott ◽  
Jean Russell ◽  
Thomas Klonisch ◽  
Sabine Hombach-Klonisch ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
In Vitro Model ◽  
Epithelial Cell Line ◽  
Prostaglandin E ◽  
Sperm Viability ◽  
Cell Passage ◽  
In Vitro System ◽  
Vitro Model

In vivo, gamete maturation, fertilisation and early embryonic development take place inside the oviduct. Several studies have indicated that local responses towards gametes and embryos are generated by the maternal reproductive tract. However, no defined in vitro model currently exists to allow detailed and systematic investigation of maternal communications with gametes and embryos. Therefore, we characterised an in vitro model based on the interaction of boar spermatozoa with an immortalised porcine oviduct epithelial cell line to evaluate different factors that may affect this model. The factors tested were sperm viability, source of spermatozoa, cell passage effect and the effect of reproductive and non-reproductive epithelial cells in the interaction with spermatozoa. After 24 h of co-incubation, RNA was extracted and used to synthesise cDNA for quantitative real-time PCR. Alteration in the expression of genes such as adrenomedullin, heat-shock 70-kDa protein 8 and prostaglandin E synthase was considered as the end point of this assay. The results showed that sperm viability and cell passage number had an effect on oviductal gene expression in response to spermatozoa. Oviductal cells showed significant alterations in gene expression when compared with non-reproductive epithelial cells. The simple in vitro system described here has potential application for further studies in our understanding of mechanisms involved in maternal interactions with spermatozoa.

An In Vitro Model for Tumor Progression in Murine Lymphoid Cells

1986 ◽  
pp. 75-80 ◽  
Author(s):  
Richard C. Schwartz ◽  
Lawrence W. Stanton ◽  
Kenneth B. Marcu ◽  
Owen N. Witte
Keyword(s):  
Tumor Progression ◽  
In Vitro Model ◽  
Lymphoid Cells ◽  
Vitro Model

Interleukin-8 stimulates the migration of human colonic epithelial cells in vitro

1999 ◽  
Vol 97 (3) ◽  
pp. 385-390 ◽  
Author(s):  
Andrew J. WILSON ◽  
Keith BYRON ◽  
Peter R. GIBSON
Keyword(s):  
Cell Migration ◽  
Epithelial Cells ◽  
In Vitro Model ◽  
Interleukin 8 ◽  
Colonic Epithelium ◽  
Dependent Manner ◽  
Colonic Epithelial Cells ◽  
Tnf Α ◽  
Vitro Model

The migration of colonic epithelial cells (restitution) is an important event in the repair of mucosal injuries. Interleukin-8 (IL-8) is a physiological initiator of the chemotactic migration of leucocytes. This study aimed to determine whether IL-8 had a similar effect on migration in an in vitro model of wounded colonic epithelium. Cell migration over 24 h was assessed in circular wounds made in confluent monolayers of the human colon cancer cell line LIM1215. This migration was stimulated in a concentration-dependent manner by IL-8, with maximal effects of approx. 1.75-fold above basal migration. The motogenic effect of IL-8 was mediated independently of effects on cell proliferation. In contrast, it was partially dependent upon gene transcription and protein synthesis and involved the activation of pertussis-toxin-sensitive G-proteins. The short-chain fatty acids, acetate, propionate, butyrate and valerate, the activator of protein kinase C (phorbol-12-myristate-13-acetate) and tumour necrosis factor-α (TNF-α) all stimulated the secretion of IL-8. However, only the motogenic effect of TNF-α was dependent upon IL-8. In conclusion, IL-8 stimulated cell migration in an in vitro model of colonic epithelium, whereas the motogenic effect of at least one physiologically relevant factor was dependent upon an increase in its endogenous levels. If IL-8 stimulates colonic epithelial restitution in vivo, this would have ramifications for the control of repair processes following wounding of the colonic mucosa.

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