The Role of Methylation in Regulating the Expression of the Alpha-Fetoprotein Gene in Developing Rat Liver and Hepatoma Cell Lines

1989 ◽  
Vol 2 (5) ◽  
pp. 287-297 ◽  
Author(s):  
Catharine L. Smith ◽  
Peter W. Nordloh ◽  
Jen-Fu Chiu
Keyword(s):  
Cell Lines ◽  
Rat Liver ◽  
Hepatoma Cell ◽  
Alpha Fetoprotein ◽  
Hepatoma Cell Lines ◽  
Developing Rat

Different Expression of Esterase Variants in Rat Hepatic and Hepatom a-Derived Cell Lines Detected by Electrophoresis

1995 ◽  
Vol 50 (9-10) ◽  
pp. 664-668 ◽  
Author(s):  
Adel S. Afify ◽  
Yoshimitsu Yamazaki ◽  
Yu-ichi Kageyama ◽  
Shiro Yusa ◽  
Yoshikatsu Ogawa ◽  
...  
Keyword(s):  
Cell Lines ◽  
Rat Liver ◽  
Hepatoma Cell ◽  
Polyacrylamide Gel Electrophoresis ◽  
Liver Homogenate ◽  
Normal Liver ◽  
Liver Parenchyma ◽  
Hepatoma Cell Lines ◽  
Normal Rat ◽  
Transformed Cell Lines

Abstract Esterases in nine rat hepatic and hepatoma-derived cell lines and normal rat liver homogenate were detected by polyacrylamide gel electrophoresis coupled with active staining with a-naphthyl acetate or butyrate as a substrate. The esterase band patterns of the non-cancerous and oncogene-transformed cell lines were alike, but different from those of hepatoma cell lines and normal rat liver homogenate. The former groups of cells might have completely lost the characteristics of rat liver parenchymal cells, or else they might have their origin at cells other than liver parenchyma. The esterase patterns of the hepatoma cell lines (e.g., McA-RH7777) and the normal rat liver highly resembled with each other, exemplifying the slight biochemical deviation of cancer from normal cells. However, two-dimensional electrophoretogram for the McA-RH7777 cell line showed a prominent esterase spot {p/ 6.0-Mr 110 kDa) that was lacking in the normal liver. This result indicates that there is invariably some change in esterase expression between the cancer cells and the normal liver cells

Molecular cloning of cDNA for rat argininosuccinate lyase and its expression in rat hepatoma cell lines

1986 ◽  
Vol 6 (5) ◽  
pp. 1722-1728
Author(s):  
M A Lambert ◽  
L R Simard ◽  
P N Ray ◽  
R R McInnes
Keyword(s):  
Cell Lines ◽  
Rat Liver ◽  
Transcriptional Activity ◽  
Hepatoma Cell ◽  
Cdna Clones ◽  
Argininosuccinate Lyase ◽  
Hepatoma Cell Lines ◽  
Liver Cdna Library ◽  
Nuclear Processing ◽  
Rat Hepatoma

Using antibody and plaque hybridization screening, we isolated rat argininosuccinate lyase (AS lyase) cDNA clones from a liver cDNA library prepared in the phage expression vector lambda gt11. Five overlapping cDNAs covering 1.7 kilobases of the estimated 2.0-kilobase AS lyase mRNA were characterized and confirmed as AS lyase sequences by hybrid selection. We examined the differential expression of AS lyase in rat liver and four rat hepatoma cell lines (7800C1, H4, HTC, and MH1C1). These cells exhibited a 60-fold range of AS lyase enzyme activity, with a direct correlation between activity, amount of AS lyase immunoreactive protein, and quantity of specific AS lyase mRNA. These observations suggest that the differences in AS lyase expression between rat liver and the hepatoma cell lines result from variations in AS lyase transcriptional activity or alterations in nuclear processing of AS lyase RNA.

Nuclear Oncogenes and Alpha-Fetoprotein Gene Expression in Hepatoma Cell Lines

Tumor Biology ◽  
1993 ◽  
Vol 14 (4) ◽  
pp. 201-212 ◽  
Author(s):  
Isabelle Tournier-Thurneyssen ◽  
Gérard Feldmann ◽  
Dominique Bernuau
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Hepatoma Cell ◽  
Alpha Fetoprotein ◽  
Hepatoma Cell Lines

Albumin and alpha-fetoprotein gene transcription in rat hepatoma cell lines is correlated with specific DNA hypomethylation and altered chromatin structure in the 5' region

1987 ◽  
Vol 7 (5) ◽  
pp. 1856-1864
Author(s):  
I Tratner ◽  
J L Nahon ◽  
J M Sala-Trepat ◽  
A Venetianer
Keyword(s):  
Cell Lines ◽  
Chromatin Structure ◽  
Hepatoma Cell ◽  
Dnase I ◽  
Alpha Fetoprotein ◽  
Hepatoma Cell Lines ◽  
Dnase I Hypersensitive Sites ◽  
Albumin Gene ◽  
Hypersensitive Sites ◽  
Rat Hepatoma

We examined DNA methylation and DNase I hypersensitivity of the alpha-fetoprotein (AFP) and albumin gene region in hepatoma cell lines which showed drastic differences in the level of expression of these genes. We assayed for methylation of the CCGG sequences by using the restriction enzyme isoschizomers HpaII and MspI. We found two methylation sites located in the 5' region of the AFP gene and one in exon 1 of the albumin gene for which hypomethylation is correlated with gene expression. Another such site, located about 4,000 base pairs upstream from the AFP gene, seems to be correlated with the tissue specificity of the cells. DNase I-hypersensitive sites were mapped by using the indirect end-labeling technique with cloned genomic DNA probes. Three tissue-specific DNase I-hypersensitive sites were mapped in the 5' flanking region of the AFP gene when this gene was transcribed. Similarly, three tissue-specific DNase I-hypersensitive sites were detected upstream from the albumin gene in producing cell lines. In both cases, the most distal sites were maintained after cessation of gene activity and appear to be correlated with the potential expression of the gene. Interestingly, specific methylation sites are localized in the same DNA region as DNase I hypersensitive sites. This suggests that specific alterations of chromatin structure and changes in methylation pattern occur in specific critical regulatory regions upstream from the albumin and AFP genes in rat hepatoma cell lines.

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