scholarly journals Novel mechanistic targets of forkhead box Q1 transcription factor in human breast cancer cells

2020 ◽  
Vol 59 (10) ◽  
pp. 1116-1128
Author(s):  
Su‐Hyeong Kim ◽  
Eun‐Ryeong Hahm ◽  
Krishna B. Singh ◽  
Shivendra V. Singh
Keyword(s):  
Breast Cancer ◽  
Transcription Factor ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Forkhead Box

scholarly journals The Brn-3b POU family transcription factor represses plakoglobin gene expression in human breast cancer cells

2005 ◽  
Vol 118 (4) ◽  
pp. 869-878 ◽  
Author(s):  
Laila Samady ◽  
David J. Faulkes ◽  
Vishwanie Budhram-Mahadeo ◽  
Daniel Ndisang ◽  
Eyck Potter ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Transcription Factor ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast

scholarly journals Novel mechanistic targets of Forkhead box Q1 transcription factor in human breast cancer cells

2020 ◽  
Author(s):  
Su-Hyeong Kim ◽  
Eun-Ryeong Hahm ◽  
Krishna B. Singh ◽  
Shivendra V. Singh
Keyword(s):  
Breast Cancer ◽  
Transcription Factor ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Rna Seq ◽  
Human Breast ◽  
Forkhead Box ◽  
Luminal Type ◽  
Mcf 7

The transcription factor forkhead box Q1 (FoxQ1), which is overexpressed in different solid tumors, has emerged as a key player in the pathogenesis of breast cancer by regulating epithelial-mesenchymal transition, maintenance of cancer-stem like cells, and metastasis. However, the mechanism underlying oncogenic function of FoxQ1 is still not fully understood. In this study, we compared the RNA-seq data from FoxQ1 overexpressing SUM159 cells with that of empty vector-transfected control (EV) cells to identify novel mechanistic targets of this transcription factor. Consistent with published results in basal-like subtype, immunohistochemistry revealed upregulation of FoxQ1 protein in luminal-type human breast cancer tissue microarrays when compared to normal mammary tissues. Many previously reported transcriptional targets of FoxQ1 (e.g., E-cadherin, N-cadherin, fibronectin 1, etc.) were verified from the RNA-Seq analysis. FoxQ1 overexpression resulted in downregulation of genes associated with cell cycle checkpoints, M phase, and cellular response to stress/external stimuli as evidenced from the Reactome pathway analysis. Consequently, FoxQ1 overexpression resulted in S, G2M and mitotic arrest in basal-like SUM159 and HMLE cells, but not in luminal-type MCF-7 cells. There were differences in expression of cell cycle-associated proteins between FoxQ1 overexpressing SUM159 and MCF-7 cells. Finally, we show for the first time that FoxQ1 is a direct transcriptional regulator of interleukin (IL)-1α, IL-8, and vascular endothelial growth factor in breast cancer cells. Chromatin immunoprecipitation revealed FoxQ1 occupancy at the promoters of IL-1α, IL-8, and VEGF. In conclusion, the present study reports novel mechanistic targets of FoxQ1 in human breast cancer cells.

scholarly journals miR-381 suppresses C/EBPα-dependent Cx43 expression in breast cancer cells

2015 ◽  
Vol 35 (6) ◽  
Author(s):  
Jia Ming ◽  
Yan Zhou ◽  
Junze Du ◽  
Shenghao Fan ◽  
Beibei Pan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Transcription Factor ◽  
Metastatic Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Metastatic Breast ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Cx43 Expression

miR-381 suppressed CX43 expression by directly targeting the 3′-UTR of C/EBPα, a novel transcription factor of Cx43 in human breast cancer cells. The miR-381–Cx43 axis might be a useful diagnostic and therapeutic target of metastatic breast cancer.

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