scholarly journals Effects of supplemental calcium and vitamin D on tight‐junction proteins and mucin‐12 expression in the normal rectal mucosa of colorectal adenoma patients

2019 ◽  
Author(s):  
Hannah B. Mandle ◽  
Ferdous A. Jahan ◽  
Roberd M. Bostick ◽  
John A. Baron ◽  
Elizabeth L. Barry ◽  
...  
Keyword(s):  
Vitamin D ◽  
Tight Junction ◽  
Colorectal Adenoma ◽  
Rectal Mucosa ◽  
Tight Junction Proteins ◽  
Supplemental Calcium ◽  
Junction Proteins

scholarly journals Vitamin D3 induces vitamin D receptor and HDAC11 binding to relieve the promoter of the tight junction proteins

Oncotarget ◽  
2017 ◽  
Vol 8 (35) ◽  
pp. 58781-58789 ◽  
Author(s):  
Feng-Hua Liu ◽  
Shan-Shan Li ◽  
Xiao-Xi Li ◽  
Shuai Wang ◽  
Mao-Gang Li ◽  
...  
Keyword(s):  
Vitamin D ◽  
Tight Junction ◽  
Vitamin D Receptor ◽  
Vitamin D3 ◽  
Tight Junction Proteins ◽  
Junction Proteins

The Combined Effect of Vitamin C and Vitamin D3 on the Intestinal Epithelial Barrier by Regulating Notch Signaling Pathway

2019 ◽  
Author(s):  
Fubin Qiu ◽  
Zehui Zhang ◽  
Ying Ma ◽  
Linxue Yang ◽  
Rui Li
Keyword(s):  
Vitamin D ◽  
Vitamin C ◽  
Tight Junction ◽  
Mucosal Barrier ◽  
Control Group ◽  
High Dose ◽  
Tight Junction Proteins ◽  
Intestinal Mucosal Barrier ◽  
Notch Signal ◽  
Junction Proteins

Abstract Background: Tight junction proteins play crucial role in maintaining the intestinal mucosal barrier. Although previous studies had shown that the Notch signal is closely related to tight junction proteins, the mechanism by which it does so remains unknown. The goal of the present study was to investigate whether vitamin C combined with vitamin D3 affects intestinal mucosal barrier stability through Notch signal pathway.Results: To assess the effect of vitamin C combined with vitamin D3 on the intestinal mucosal barrier, electron microscopic observation of ultrastructure of tight junctions was done. And tight junction proteins gene and Notch signal gene expression were analyzed by quantitative reverse-transcription polymerase chain reaction, expression of tight junction protein in SW480 cells interfered with by LPS were examined by western blot. We found that vitamin C combined with vitamin D3 had protective effect on DSS-induced ulcerative colitis in guinea pig intestinal mucosa. Electron microscopy results showed that both low dose and high dose of vitamin C combined with vitamin D3 could maintain DSS-induced ulcerative colitis in guinea pig intestinal epithelium tight junction, however, the combination of medium dose vitamin C and vitamin D3 did not have this effect; Compared with the control group, the expression level of ZO-1 mRNA in the colon tissue of high-dose vitamin C group was significantly increased. In SW480 cell experiments, compared with the control group, the cell migration and repair ability of different concentrations of vitamin C combined with vitamin D3 group were significantly improved, the protein expression of Notch-1 was increased, but the protein expression of claudin-2 was significantly decreased. Conclusions: our results of this experiment showed that the appropriate amount of vitamin C combined with vitamin D3 might regulate the expression of claudin-2 by regulating Notch-1, slow the intestinal mucosal barrier destruction, and promote the damage repair of cell mucosal barrier.

scholarly journals Effects of Supplemental Calcium and Vitamin D on Expression of Toll-Like Receptors and Phospho-IKKα/β in the Normal Rectal Mucosa of Colorectal Adenoma Patients

2018 ◽  
Vol 11 (11) ◽  
pp. 707-716 ◽  
Author(s):  
Rebecca Hodge ◽  
Hannah B. Mandle ◽  
Stephen Ray ◽  
Sonia Tandon ◽  
Meaghan Peterson ◽  
...  
Keyword(s):  
Vitamin D ◽  
Colorectal Adenoma ◽  
Rectal Mucosa ◽  
Toll Like Receptors ◽  
Supplemental Calcium

scholarly journals Tight Junction Proteins Claudin-2 and -12 Are Critical for Vitamin D-dependent Ca2+ Absorption between Enterocytes

2008 ◽  
Vol 19 (5) ◽  
pp. 1912-1921 ◽  
Author(s):  
Hiroki Fujita ◽  
Kotaro Sugimoto ◽  
Shuichiro Inatomi ◽  
Toshihiro Maeda ◽  
Makoto Osanai ◽  
...  
Keyword(s):  
Vitamin D ◽  
Tight Junction ◽  
Active Form ◽  
Intestinal Epithelial ◽  
Tight Junction Proteins ◽  
Dihydroxyvitamin D ◽  
Intestinal Epithelia ◽  
Junction Proteins

Ca2+ is absorbed across intestinal epithelial monolayers via transcellular and paracellular pathways, and an active form of vitamin D3, 1α,25-dihydroxyvitamin D3 [1α,25(OH)2D3], is known to promote intestinal Ca2+ absorption. However, the molecules driving the paracellular Ca2+ absorption and its vitamin D dependency remain obscure. Because the tight junction proteins claudins are suggested to form paracellular channels for selective ions between neighboring cells, we hypothesized that specific intestinal claudins might facilitate paracellular Ca2+ transport and that expression of these claudins could be induced by 1α,25(OH)2D3. Herein, we show, by using RNA interference and overexpression strategies, that claudin-2 and claudin-12 contribute to Ca2+ absorption in intestinal epithelial cells. We also provide evidence showing that expression of claudins-2 and -12 is up-regulated in enterocytes in vitro and in vivo by 1α,25(OH)2D3 through the vitamin D receptor. These findings strongly suggest that claudin-2- and/or claudin-12-based tight junctions form paracellular Ca2+ channels in intestinal epithelia, and they highlight a novel mechanism behind vitamin D-dependent calcium homeostasis.

Tight junction proteins in HCC and metastatic liver tumours

2005 ◽  
Vol 43 (05) ◽  
Author(s):  
Cs Páska ◽  
E Orbán ◽  
A Kiss ◽  
Zs Schaff ◽  
A Szijjártó ◽  
...  
Keyword(s):  
Tight Junction ◽  
Tight Junction Proteins ◽  
Liver Tumours ◽  
Metastatic Liver ◽  
Junction Proteins

Evidence that gene expression of ovarian follicular tight junction proteins is regulated in vivo and in vitro in cattle

2017 ◽  
Vol 95 (3) ◽  
pp. 1313 ◽  
Author(s):  
L. Zhang ◽  
L. F. Schütz ◽  
C. L. Robinson ◽  
M. L. Totty ◽  
L. J. Spicer
Keyword(s):  
Gene Expression ◽  
Tight Junction ◽  
Tight Junction Proteins ◽  
Junction Proteins
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