Notch3-specific inhibition using siRNA knockdown or GSI sensitizes paclitaxel-resistant ovarian cancer cells

2015 ◽  
Vol 55 (7) ◽  
pp. 1196-1209 ◽  
Author(s):  
Haeyoun Kang ◽  
Ju-Yeon Jeong ◽  
Ji-Ye Song ◽  
Tae Heon Kim ◽  
Gwangil Kim ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Specific Inhibition ◽  
Sirna Knockdown

scholarly journals NRF2 Regulates HER1 Signaling Pathway to Modulate the Sensitivity of Ovarian Cancer Cells to Lapatinib and Erlotinib

2017 ◽  
Vol 2017 ◽  
pp. 1-19 ◽  
Author(s):  
Ibrahim H. Kankia ◽  
Hilal S. Khalil ◽  
Simon P. Langdon ◽  
Peter R. Moult ◽  
James L. Bown ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Cellular Proliferation ◽  
Ovarian Cancer Cells ◽  
Epidermal Growth Factor Receptors ◽  
Related Factor ◽  
Her Family ◽  
Epidermal Growth ◽  
Nrf2 Activator ◽  
Sirna Knockdown

NF-E2-related factor 2 (NRF2) regulates the transcription of a battery of metabolic and cytoprotective genes. NRF2 and epidermal growth factor receptors (EGFRs/HERs) are regulators of cellular proliferation and determinants of cancer initiation and progression. NRF2 and HERs confer cancers with resistance to several therapeutic agents. Nevertheless, there is limited understanding of the regulation of HER expression and activation and the link between NRF2 and HER signalling pathways. We show that NRF2 regulates both basal and inducible expression ofHER1, as treatment of ovarian cancer cells (PEO1, OVCAR3, and SKOV3) with NRF2 activator tBHQ inducingHER1, while inhibition of NRF2 by siRNA knockdown or with retinoid repressesHER1. Furthermore, treatment of cells with tBHQ increased total and phosphorylated NRF2, HER1, and AKT levels and compromised the cytotoxic effect of lapatinib or erlotinib. Treatment with siRNA or retinoid antagonised the effect of tBHQ on NRF2 and HER1 levels and enhanced the sensitivity of ovarian cancer cells to lapatinib or erlotinib. Pharmacological or genetic inhibition of NRF2 and/or treatment with lapatinib or erlotinib elevated cellular ROS and depleted glutathione. This extends the understanding of NRF2 and its regulation of HER family receptors and opens a strategic target for improving cancer therapy.

Cytotoxic effects of disulfiram and synergism with cisplatin on ovarian cancer cells in vitro

2018 ◽  
Author(s):  
F Guo ◽  
Z Yang ◽  
J Xu ◽  
J Sehouli ◽  
AE Albers ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Cytotoxic Effects
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