Wogonin reverses hypoxia resistance of human colon cancer HCT116 cells via downregulation of HIF-1α and glycolysis, by inhibiting PI3K/Akt signaling pathway

2013 ◽  
Vol 53 (S1) ◽  
pp. E107-E118 ◽  
Author(s):  
Hu Wang ◽  
Li Zhao ◽  
Li-Tao Zhu ◽  
Yu Wang ◽  
Di Pan ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Signaling Pathway ◽  
Human Colon ◽  
Akt Signaling ◽  
Akt Signaling Pathway ◽  
Human Colon Cancer ◽  
Hct116 Cells

Lycopene inhibits growth of human colon cancer cellsvia suppression of the Akt signaling pathway

2008 ◽  
Vol 52 (6) ◽  
pp. 646-654 ◽  
Author(s):  
Feng-Yao Tang ◽  
Chung-Jin Shih ◽  
Li-Hao Cheng ◽  
Hsin-Jung Ho ◽  
Hung-Jiun Chen
Keyword(s):  
Colon Cancer ◽  
Signaling Pathway ◽  
Human Colon ◽  
Akt Signaling ◽  
Akt Signaling Pathway ◽  
Human Colon Cancer

scholarly journals UNBS5162 inhibits colon cancer growth via suppression of PI3K/Akt signaling pathway

2018 ◽  
Vol 34 ◽  
pp. 99-104 ◽  
Author(s):  
Fan Zhang ◽  
Hui-zeng Lv ◽  
Ji-ming Liu ◽  
Xiao-yong Ye ◽  
Cun-chuan Wang
Keyword(s):  
Colon Cancer ◽  
Western Blot ◽  
Signaling Pathway ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Underlying Mechanism ◽  
Akt Signaling ◽  
Expression Level ◽  
Akt Signaling Pathway ◽  
Hct116 Cells

Colon cancer is a common cause of cancer-related death worldwide. However, the underlying mechanism of tumor progression of colon cancer remains far from being elucidated. In the present study, we report the role of UNBS5162 in colon cancer. UNBS5162 is a naphthalimide that can intercalate into DNA and suppress the expression level of CXCL chemokines. Here, we investigated its effect on cell proliferation, mobility and apoptosis in HCT116 cells, and explored the underlying mechanism. A CCK8 assay revealed that UNBS5162 can block the proliferation of colon cancer cells. Base on a Transwell assay, we showed that cell migration and invasion ability of HCT116 cells are inhibited by UNBS5162. In addition, Annexin V-FITC/PI assay and Western blot analysis were performed to detect whether UNBS5162 could induce cell apoptosis. The results indicated that UNBS5162 increases the number of apoptotic cells remarkably. Furthermore, Western blot analysis demonstrated that UNBS5162 down-regulates the expression level of Bcl2, and up-regulates that of Bax as well as the level of activated Caspase-3. Moreover, we examined the impact of UNBS5162 on PI3K/Akt signaling pathway. UNBS5162 substantially inhibited the phosphorylation of Akt and its downstream effector mTOR, and reduced the expression of p-70. Taken together, these results suggest that UNBS5162 should be considered as a potent therapeutic anticancer agent that targets the PI3K/AKT signaling pathway.

scholarly journals Astragalin Inhibits the Proliferation and Migration of Human Colon Cancer HCT116 Cells by Regulating the NF-κB Signaling Pathway

2021 ◽  
Vol 12 ◽  
Author(s):  
Min Yang ◽  
Wen-Yun Li ◽  
Jing Xie ◽  
Zi-Lin Wang ◽  
Yan-Long Wen ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Signaling Pathway ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
And Migration ◽  
Hct116 Cells ◽  
Proliferation And Migration

Astragalin is a flavonoid found in a variety of natural plants. It has anti-inflammatory, anti-oxidant effects and has inhibited effects against several malignant tumor cell types. However, its effects on colon cancer and the molecular mechanisms have remained to be elucidated. In this study, we evaluated the inhibitory effect of astragalin on proliferation and migration of human colon cancer HCT116 cells in vitro and in vivo. Furthermore, we elucidated the mechanism of these effects. The results showed that astragalin significantly inhibited the proliferation and diffusion of HCT116 cells by induced apoptosis (by modulation of Bax, Bcl-2, P53, caspase-3, caspase 6, caspase 7, caspase 8, caspase 9 protein express) and cell cycle arrest (by modulation of Cyclin D1, Cyclin E, P21, P27, CDK2, CDK4 protein express). Moreover, astragalin suppressed HCT116 cell migration by inhibiting the expression of matrix metalloproteinases (MMP-2, MMP-9). In addition, astragalin significantly downregulated the expression of key proteins in the NF-κB signaling pathway and inhibited the transcriptional activity of NF-κB P65 stimulated with inflammatory cytokines TNF-α, thereby inhibiting the growth of colon cancer cells in vitro. Our further investigations unveiled astragalin gavage significantly reduced the proliferation of colon cancer xenograft in nude mice, in vivo experiments showed that tumor growth was related to decreased expression of apoptotic proteins in tumor tissues and decreased activity of the NF-κB signaling pathway. In summary, our results indicated that astragalin inhibits the proliferation and growth of colon cancer cells in vivo and in vitro via the NF-κB pathway. Therefore, astragalin maybe become a potential plant-derived antitumor drug for colon cancer.

scholarly journals Deoxycholyltaurine rescues human colon cancer cells from apoptosis by activating EGFR-dependent PI3K/Akt signaling

2008 ◽  
Vol 215 (2) ◽  
pp. 538-549 ◽  
Author(s):  
Jean-Pierre Raufman ◽  
Jasleen Shant ◽  
Chang Yue Guo ◽  
Sanjit Roy ◽  
Kunrong Cheng
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Colon ◽  
Akt Signaling ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells

Abstract 2024: Studying the effects of interfering with doxorubicin-induced senescence in human colon cancer HCT116 cells

2021 ◽  
Author(s):  
Moureq Rashed Alotaibi ◽  
Homood As Sobeia ◽  
Munirah Alohaydib ◽  
Faten Alaqil ◽  
Khalid Alhazzani ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Human Colon ◽  
Human Colon Cancer ◽  
Hct116 Cells
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