scholarly journals Enhanced skin carcinogenesis and lack of thymus hyperplasia in transgenic mice expressing human cyclin D1b (CCND1b)

2009 ◽  
Vol 48 (6) ◽  
pp. 508-516 ◽  
Author(s):  
Paola Rojas ◽  
Fernando Benavides ◽  
Jorge Blando ◽  
Carlos Perez ◽  
Kim Cardenas ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Skin Carcinogenesis ◽  
Thymus Hyperplasia

scholarly journals Enhanced UV-Induced Skin Carcinogenesis in Transgenic Mice Overexpressing Proprotein Convertases

Neoplasia ◽  
2013 ◽  
Vol 15 (2) ◽  
pp. 169-179 ◽  
Author(s):  
Jian Fu ◽  
Daniel E. Bassi ◽  
Jirong Zhang ◽  
Tianyu Li ◽  
Kathy Q. Cai ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Skin Carcinogenesis ◽  
Proprotein Convertases

scholarly journals Increased Incidence of Squamous Cell Carcinomas in Mastomys natalensis Papillomavirus E6 Transgenic Mice during Two-Stage Skin Carcinogenesis

2004 ◽  
Vol 78 (9) ◽  
pp. 4797-4805 ◽  
Author(s):  
Iris Helfrich ◽  
Min Chen ◽  
Rainer Schmidt ◽  
Gerhard Fürstenberger ◽  
Annette Kopp-Schneider ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Squamous Cell ◽  
Malignant Tumors ◽  
Squamous Cell Carcinomas ◽  
Skin Carcinogenesis ◽  
Rank Test ◽  
Causative Role ◽  
Mastomys Natalensis ◽  
Two Stage ◽  
Papillomavirus Infection

ABSTRACT Papillomaviruses cause certain forms of human cancers, most notably carcinomas of the uterine cervix. In contrast to the well-established involvement of papillomavirus infection in the etiology of cervical carcinomas and in carcinomas of a rare hereditary condition, epidermodysplasia verruciformis, a causative role for cutaneous human papillomavirus types in the development of nonmelanoma skin cancer has not been proven. In order to better understand the functions of individual genes of cutaneous papillomavirus types, we generated transgenic mice carrying oncogene E6 of the Mastomys natalensis papillomavirus (MnPV), which causes keratoacanthomas of the skin in its natural host. In the present study, we demonstrate that under conditions of experimental two-stage skin carcinogenesis, fast-paced squamous cell carcinomas develop in nearly 100% of MnPV E6 transgenic mice in comparison to 10% in their nontransgenic littermates (log rank test; P < 0.0001). Therefore, we conclude that the MnPV E6 transgene favors the malignant progression of chemically induced tumors. Whereas an activating H-ras mutation is a consistent feature in benign and malignant tumors in wild-type mice, the majority of papillomas and keratoacanthomas and all squamous cell carcinomas obtained in MnPV E6 transgenic mice contain nonmutated ras alleles. These results indicate that the development of squamous cell carcinomas in MnPV E6 transgenic mice does not depend on an activated H-ras oncogene.

scholarly journals Bioactivity of betulinic acid nanoemulsions on skin carcinogenesis in transgenic mice K14E6

2022 ◽  
Vol 72 (4) ◽  
pp. e433
Author(s):  
B. Agame-Lagunes ◽  
M. Alegria-Rivadeneyra ◽  
A. Alexander-Aguilera ◽  
R. Quintana-Castro ◽  
C. Torres-Palacios ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acids ◽  
Transgenic Mice ◽  
Cancer Treatment ◽  
Murine Model ◽  
Betulinic Acid ◽  
Tumor Development ◽  
Alternative Therapies ◽  
Skin Carcinogenesis ◽  
Free Form

Alternative therapies for cancer treatment have been developed using bioactive compounds such as betulinic acid (BA). The objective of this study was to investigate the bioactivity of BA in its free form and compare it with its nano-encapsulated form under a skin carcinogenesis protocol in a genetically modified murine model. K14E6 and FVB mice were divided into four groups to be treated with free BA and with betulinic acid nanoemulsion (BANE). Lecithin enriched with medium chain fatty acids (MCFAs) was employed as an emulsifier to prepare the nanoemulsions with a mean droplet size of 40 nm. Skin tumors were induced by exposure to DMBA and TPA directly to the transgenic mice. Tumor development was completely inhibited by BANE and by 70% with free BA. This was validated by histological sections and the gene expression of the Cdk4 and Casp8 genes.

Homozygous K5Cre transgenic mice have wavy hair and accelerated malignant progression in a murine model of skin carcinogenesis

2006 ◽  
Vol 46 (1) ◽  
pp. 49-59 ◽  
Author(s):  
Edward L. Chan ◽  
Belinda E. Peace ◽  
Kenya Toney ◽  
Sarah A. Kader ◽  
Peterson Pathrose ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Murine Model ◽  
Skin Carcinogenesis ◽  
Malignant Progression

scholarly journals Unimpeded skin carcinogenesis in K14-HPV16 transgenic mice deficient for plasminogen activator inhibitor

2010 ◽  
Vol 128 (2) ◽  
pp. 283-293 ◽  
Author(s):  
Anne Masset ◽  
Catherine Maillard ◽  
Nor Eddine Sounni ◽  
Nathalie Jacobs ◽  
Françoise Bruyére ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Plasminogen Activator ◽  
Plasminogen Activator Inhibitor ◽  
Skin Carcinogenesis ◽  
Activator Inhibitor

scholarly journals Thrombospondin-2 overexpression in the skin of transgenic mice reduces the susceptibility to chemically induced multistep skin carcinogenesis

2014 ◽  
Vol 74 (2) ◽  
pp. 106-115 ◽  
Author(s):  
Rainer Kunstfeld ◽  
Thomas Hawighorst ◽  
Michael Streit ◽  
Young-Kwon Hong ◽  
Lynh Nguyen ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Skin Carcinogenesis ◽  
Chemically Induced

scholarly journals RasGRP1 Overexpression in the Epidermis of Transgenic Mice Contributes to Tumor Progression during Multistage Skin Carcinogenesis

2007 ◽  
Vol 67 (21) ◽  
pp. 10190-10197 ◽  
Author(s):  
Courtney T. Luke ◽  
Carolyn E. Oki-Idouchi ◽  
J. Mark Cline ◽  
Patricia S. Lorenzo
Keyword(s):  
Transgenic Mice ◽  
Tumor Progression ◽  
Skin Carcinogenesis

scholarly journals CYLD Inhibits the Development of Skin Squamous Cell Tumors in Immunocompetent Mice

2021 ◽  
Vol 22 (13) ◽  
pp. 6736
Author(s):  
Josefa P. Alameda ◽  
Verónica A. García-García ◽  
Silvia López ◽  
Ana Hernando ◽  
Angustias Page ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Tumor Suppressor ◽  
Transgenic Mice ◽  
Tumor Development ◽  
Skin Carcinogenesis ◽  
Moderate Increase ◽  
Wild Type ◽  
Suppressor Activity ◽  
Immunocompetent Mice

Cylindromatosis (CYLD) is a deubiquitinase (DUB) enzyme that was initially characterized as a tumor suppressor of adnexal skin tumors in patients with CYLD syndrome. Later, it was also shown that the expression of functionally inactive mutated forms of CYLD promoted tumor development and progression of non-melanoma skin cancer (NMSC). However, the ability of wild-type CYLD to inhibit skin tumorigenesis in vivo in immunocompetent mice has not been proved. Herein, we generated transgenic mice that express the wild type form of CYLD under the control of the keratin 5 (K5) promoter (K5-CYLDwt mice) and analyzed the skin properties of these transgenic mice by WB and immunohistochemistry, studied the survival and proliferating characteristics of primary keratinocytes, and performed chemical skin carcinogenesis experiments. As a result, we found a reduced activation of the nuclear factor kappa B (NF-κB) pathway in the skin of K5-CYLDwt mice in response to tumor necrosis factor-α (TNF-α); accordingly, when subjected to insults, K5-CYLDwt keratinocytes are prone to apoptosis and are protected from excessive hyperproliferation. Skin carcinogenesis assays showed inhibition of tumor development in K5-CYLDwt mice. As a mechanism of this tumor suppressor activity, we found that a moderate increase in CYLD expression levels reduced NF-κB activation, which favored the differentiation of tumor epidermal cells and inhibited its proliferation; moreover, it decreased tumor angiogenesis and inflammation. Altogether, our results suggest that increased levels of CYLD may be useful for anti-skin cancer therapy.

Sign in / Sign up

Export Citation Format

Share Document