Background and Purpose: It has been well-known both gold nanoparticles (AuNPs) and cisplatin are
potential radiosensitizers for radiotherapy of cancer. In this in vitro study, we investigated the chemoradiotherapeutic
effects of alginate nanogel co-loaded with AuNPs and cisplatin (ACA) on U87-MG human
glioblastoma cells.
Methods:
Based on the accomplished pilot studies, U87-MG cells were incubated with ACA and cisplatin at
10% inhibitory concentration (IC10) for 4h. Then, the cells were irradiated to different doses of 6MV X-rays (2
and 10 Gy). MTT assay was performed to evaluate the cell survival rate. Apoptosis was determined by flow
cytometry using an annexinV–fluorescein isothiocyanate/propidium iodide apoptosis detection kit.
Results:
The results showed that ACA at the concentration of 4 µg/ml (per cisplatin) and free cisplatin at concentration
of 15 μg/ml have the same effects on U87-MG cells (survival rate: 90%). The combination of ACA
with radiation resulted in a significant decrease in cell viability (survival rate: 30%). The flow cytometry assay
also showed that such a combination therapy induces more apoptosis than necrosis.
Conclusion:
It may be concluded that co-delivery of AuNPs and cisplatin with a single nanoplatform like ACA
nanocomplex enhances the therapeutic ratio of human glioblastoma radiation therapy.
Jinlong capsule inhibits migration and invasion in human glioblastoma cells via the modulation of mTOR/S6 signaling pathway