Effects of polyunsaturated fatty acids on prostaglandin synthesis and cyclooxygenase-mediated DNA adduct formation by heterocyclic aromatic amines in human adenocarcinoma colon cells

2004 ◽  
Vol 40 (3) ◽  
pp. 180-188 ◽  
Author(s):  
H.J.J. Moonen ◽  
Y.E.M. Dommels ◽  
M. van Zwam ◽  
M.H.M. van Herwijnen ◽  
J.C.S. Kleinjans ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Aromatic Amines ◽  
Adduct Formation ◽  
Prostaglandin Synthesis ◽  
Dna Adduct ◽  
Heterocyclic Aromatic Amines ◽  
Colon Cells

scholarly journals DNA Adduct Formation of 4-Aminobiphenyl and Heterocyclic Aromatic Amines in Human Hepatocytes

2011 ◽  
Vol 24 (6) ◽  
pp. 913-925 ◽  
Author(s):  
Gwendoline Nauwelaers ◽  
Erin E. Bessette ◽  
Dan Gu ◽  
Yijin Tang ◽  
Julie Rageul ◽  
...  
Keyword(s):  
Aromatic Amines ◽  
Adduct Formation ◽  
Human Hepatocytes ◽  
Dna Adduct ◽  
Heterocyclic Aromatic Amines

scholarly journals Polyunsaturated fatty acids modulate prostaglandin synthesis by ovine amnion cells in vitro

Reproduction ◽  
2010 ◽  
Vol 140 (6) ◽  
pp. 943-951 ◽  
Author(s):  
S E Kirkup ◽  
Z Cheng ◽  
M Elmes ◽  
D C Wathes ◽  
D R E Abayasekara
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Linolenic Acid ◽  
Stearidonic Acid ◽  
Prostaglandin Synthesis ◽  
Late Gestation ◽  
Prostaglandin E ◽  
Pufa Supplementation ◽  
Pufa Composition

Diets or supplements high in n-3 and n-6 polyunsaturated fatty acids (PUFAs) have been shown to influence the timing of parturition. PUFAs are substrates for prostaglandin (PG) synthesis, and PGs play central roles in parturition. Hence, the effects of altering PUFA composition may be mediated through alterations in the type and relative quantities of PGs synthesised. Therefore, we have investigated the effects of a range of n-3 and n-6 PUFAsin vitroon PG synthesis by amnion cells of late gestation ewes. The n-6 PUFA, arachidonic acid (20:4, n-6), increased synthesis of two-series PGs. Degree of stimulation induced by the n-6 PUFAs was dependent on the position of the PUFA in the PG synthetic pathway, i.e. PG production of the two-series (principally prostaglandin E2:PGE2) increased progressively with longer chain PUFAs. Effects of n-3 PUFAs on output of PGE2were more modest and variable. The two shorter chain n-3 PUFAs, α-linolenic acid (18:3, n-3) and stearidonic acid (18:4, n-3), induced a small but significant increase in PGE2output, while the longest chain n-3 PUFA docosahexaenoic acid (22:6, n-3) inhibited PGE2synthesis. Dihomo-γ-linolenic acid (20:3, n-6), the PUFA substrate for synthesis of one-series PGs, induced an increase in PGE1generation and a decrease in PGE2and PGE3outputs. Hence, we have demonstrated that PUFA supplementation of ovine amnion cellsin vitroaffects the type and quantity of PGs synthesised.

scholarly journals Fractionation of polyunsaturated fatty acids by urea adduct formation using supercritical CO2 as solvent.

1990 ◽  
Vol 16 (1) ◽  
pp. 38-45 ◽  
Author(s):  
Yasuo Suzuki ◽  
Masanori Konno ◽  
Kunio Arai ◽  
Shozaburo Saito
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Supercritical Co2 ◽  
Adduct Formation ◽  
Urea Adduct

scholarly journals Constructing Xenobiotic Maps of Metabolism to Predict the Role of Enzymes in DNA Adduct Formation

2021 ◽  
Author(s):  
Maël Conan ◽  
Nathalie Théret ◽  
Sophie Langouet ◽  
Anne Siegel
Keyword(s):  
Dna Adducts ◽  
Environmental Contaminants ◽  
Food Additives ◽  
Metabolic Activation ◽  
Adduct Formation ◽  
Dna Adduct ◽  
Heterocyclic Aromatic Amines ◽  
Reaction Prediction ◽  
Prediction Tools

Abstract Background : The liver plays a major role in the metabolic activation of xenobiotics (drugs, chemicals such as pollutants, pesticides, food additives...). Among environmental contaminants of concern, heterocyclic aromatic amines (HAA) are xenobiotics classified as possible or probable carcinogens (2A or 2B) by IARC for which low information exist in humans. While HAA is a family of more than thirty identified chemicals, the metabolism activation and DNA adduct formation have been fully characterized in human liver for few of them (MeIQx, PhIP, AalphaC). Results: We developed a modeling approach in order to predict all the possible metabolite derivatives of a xenobiotic. Our approach relies on the construction of an enriched and annotated map of derivative metabolites from an input metabolite. The pipeline assembles reaction prediction tools (SyGMa), sites of metabolism prediction tools (Way2Drug, SOMP and Fame 3), a tool to estimate the ability of a xenobotics to form DNA adducts (XenoSite Reactivity V1), and a filtering procedure based on Bayesian framework. This prediction pipeline was evaluated using caffeine and then applied to HAAs. The method was applied to determine enzyme profiles associated with the maximization of DNA adducts formation derived from each HAA. These profiles could be very different depending on the chemicals allowing to classify HAAs which have been grouped by their associated profiles. Conclusions: Overall, such a predictive toxicological model based on a in silico systems biology approach open perspectives to estimate genotoxicity of various chemical classes of environmental contaminants. Moreover, our approach based on enzymes profile determination open the perspective to predict various xenobiotics derived metabolites susceptible to bind DNA adducts in both normal and physiopathological situations.

The effect of aromatic amines and phenols in the thiyl-induced reactions of polyunsaturated fatty acids

2016 ◽  
Vol 124 ◽  
pp. 104-110 ◽  
Author(s):  
Ivana Tartaro Bujak ◽  
Chryssostomos Chatgilialoglu ◽  
Carla Ferreri ◽  
Luca Valgimigli ◽  
Riccardo Amorati ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Polyunsaturated Fatty Acids ◽  
Aromatic Amines
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