Regulation of cell morphology and adhesion by the tuberous sclerosis complex (TSC1/2) gene products in human kidney epithelial cells through increased E-cadherin/?-catenin activity

2003 ◽  
Vol 37 (2) ◽  
pp. 98-109 ◽  
Author(s):  
Shaowei Li ◽  
Richard Braverman ◽  
Hongzhen Li ◽  
William C. Vass ◽  
Douglas R. Lowy ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Tuberous Sclerosis ◽  
Tuberous Sclerosis Complex ◽  
Cell Morphology ◽  
Human Kidney ◽  
Gene Products ◽  
Kidney Epithelial Cells ◽  
E Cadherin

scholarly journals Identification of Noninvasive Biomarkers for Nephrotoxicity Using HK-2 Human Kidney Epithelial Cells

2014 ◽  
Vol 140 (2) ◽  
pp. 247-258 ◽  
Author(s):  
Sun Young Kim ◽  
So-Jung Sohn ◽  
A Jin Won ◽  
Hyung Sik Kim ◽  
Aree Moon
Keyword(s):  
Epithelial Cells ◽  
Human Kidney ◽  
Kidney Epithelial Cells ◽  
Noninvasive Biomarkers

Expression Profiling of Crystal-Induced Injury in Human Kidney Epithelial Cells

2006 ◽  
Vol 103 (1) ◽  
pp. p53-p62 ◽  
Author(s):  
Li Liang ◽  
Jianmin Chen ◽  
Ragini Vittal ◽  
Zachariah E. Selvanayagam ◽  
James A. McAteer ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Expression Profiling ◽  
Human Kidney ◽  
Kidney Epithelial Cells

scholarly journals Heterozygosity for the tuberous sclerosis complex (TSC) gene products results in increased astrocyte numbers and decreased p27-Kip1 expression in TSC2+/− cells

Oncogene ◽  
2002 ◽  
Vol 21 (25) ◽  
pp. 4050-4059 ◽  
Author(s):  
Erik J Uhlmann ◽  
Anthony J Apicelli ◽  
Rebecca L Baldwin ◽  
Stephen P Burke ◽  
M Livia Bajenaru ◽  
...  
Keyword(s):  
Tuberous Sclerosis ◽  
Tuberous Sclerosis Complex ◽  
Gene Products ◽  
P27 Kip1

scholarly journals Polycystin-1 and Gα12 regulate the cleavage of E-cadherin in kidney epithelial cells

2015 ◽  
Vol 47 (2) ◽  
pp. 24-32 ◽  
Author(s):  
Jen X. Xu ◽  
Tzong-Shi Lu ◽  
Suyan Li ◽  
Yong Wu ◽  
Lai Ding ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Signaling Pathway ◽  
Cell Polarity ◽  
Mdck Cells ◽  
Adherens Junction ◽  
Renal Epithelial Cells ◽  
Extracellular Milieu ◽  
Kidney Epithelial Cells ◽  
Autosomal Dominant Polycystic Kidney ◽  
E Cadherin

Interaction of polycystin-1 (PC1) and Gα12 is important for development of kidney cysts in autosomal dominant polycystic kidney disease (ADPKD). The integrity of cell polarity and cell-cell adhesions (mainly E-cadherin-mediated adherens junction) is altered in the renal epithelial cells of ADPKD. However, the key signaling pathway for this alteration is not fully understood. Madin-Darby canine kidney (MDCK) cells maintain the normal integrity of epithelial cell polarity and adherens junctions. Here, we found that deletion of Pkd1 increased activation of Gα12, which then promoted the cystogenesis of MDCK cells. The morphology of these cells was altered after the activation of Gα12. By using liquid chromatography-mass spectrometry, we found several proteins that could be related this change in the extracellular milieu. E-cadherin was one of the most abundant peptides after active Gα12 was induced. Gα12 activation or Pkd1 deletion increased the shedding of E-cadherin, which was mediated via increased ADAM10 activity. The increased shedding of E-cadherin was blocked by knockdown of ADAM10 or specific ADAM10 inhibitor GI254023X. Pkd1 deletion or Gα12 activation also changed the distribution of E-cadherin in kidney epithelial cells and caused β-catenin to shift from cell membrane to nucleus. Finally, ADAM10 inhibitor, GI254023 X, blocked the cystogenesis induced by PC1 knockdown or Gα12 activation in renal epithelial cells. Our results demonstrate that the E-cadherin/β-catenin signaling pathway is regulated by PC1 and Gα12 via ADAM10. Specific inhibition of this pathway, especially ADAM10 activity, could be a novel therapeutic regimen for ADPKD.

Metabolism of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in human kidney epithelial cells transfected with rat CYP2B1 cDNA

1993 ◽  
Vol 14 (8) ◽  
pp. 1639-1642 ◽  
Author(s):  
Dan Lacroix ◽  
Marc Desrochers ◽  
Andre Castonguay ◽  
Alan Anderson
Keyword(s):  
Epithelial Cells ◽  
Human Kidney ◽  
Kidney Epithelial Cells

SV40 transfection of human kidney epithelial cells and stability of chromosome 11

1988 ◽  
Vol 42 (6) ◽  
pp. 887-894 ◽  
Author(s):  
V. Poirier ◽  
S.J. Tyler ◽  
K.W. Brown ◽  
A.P.W. Shaw ◽  
N.J. Maitland
Keyword(s):  
Epithelial Cells ◽  
Human Kidney ◽  
Chromosome 11 ◽  
Kidney Epithelial Cells
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