scholarly journals Spotted fever group rickettsiae associated with ixodid ticks in wild environment in Southern Italy

2017 ◽  
Vol 7 (1) ◽  
pp. e00527 ◽  
Author(s):  
Donato Antonio Raele ◽  
Domenico Galante ◽  
Nicola Pugliese ◽  
Giovanna La Salandra ◽  
Maria Assunta Cafiero
Keyword(s):  
Southern Italy ◽  
Spotted Fever ◽  
Ixodid Ticks ◽  
Spotted Fever Group ◽  
Spotted Fever Group Rickettsiae

Genetic characterization of spotted fever group rickettsiae in questing ixodid ticks collected in Israel and environmental risk factors for their infection

Parasitology ◽  
2017 ◽  
Vol 144 (8) ◽  
pp. 1088-1101 ◽  
Author(s):  
JESSICA ROSE ◽  
YAARIT NACHUM-BIALA ◽  
KOSTA Y. MUMCUOGLU ◽  
MOH A. ALKHAMIS ◽  
ADI BEN-NUN ◽  
...  
Keyword(s):  
Risk Factors ◽  
Spotted Fever ◽  
Molecular Techniques ◽  
Ixodid Ticks ◽  
Geographical Information ◽  
Rickettsia Conorii ◽  
Spotted Fever Group ◽  
Gis Analysis ◽  
Spotted Fever Group Rickettsiae ◽  
Rickettsia Massiliae

SUMMARYThis study aimed to genetically characterize spotted fever group rickettsiae (SFGR) in questing ixodid ticks from Israel and to identify risk factors associated with SFGR-positive ticks using molecular techniques and geographic information systems (GIS) analysis. 1039 ticks from the genusRhipicephaluswere collected during 2014. 109/1039 (10·49%) carried SFGR-DNA of eitherRickettsia massiliae(95), ‘CandidatusRickettsia barbariae’ (8) orRickettsia conorii(6). Higher prevalence of SFGR was found inRhipicephalus turanicus(18·00%) compared withRhipicephalus sanguineussensu lato (3·22%).Rickettsia massiliaewas the most commonly detected species and the most widely disseminated throughout Israel (87·15% of allRickettsia-positive ticks). GIS analysis revealed that Central and Northern coastal regions are at high risk for SFGR. The presence of ticks was significantly associated with normalized difference vegetation index and temperature variation over the course of the year. The presence of rickettsiae was significantly associated with brown type soils, higher land surface temperature and higher precipitation. The latter parameters may contribute to infection of the tick with SFGR. Health care professionals should be aware of the possible exposure of local communities and travellers toR. massillae. Molecular and geographical information can help professionals to identify areas that are susceptible to SFGR-infected ticks.

Molecular Detection of Spotted Fever Group Rickettsiae Associated with Ixodid Ticks in Egypt

2012 ◽  
Vol 12 (5) ◽  
pp. 346-359 ◽  
Author(s):  
Sobhy Abdel-Shafy ◽  
Nesreen A.T. Allam ◽  
Oleg Mediannikov ◽  
Philippe Parola ◽  
Didier Raoult
Keyword(s):  
Molecular Detection ◽  
Spotted Fever ◽  
Ixodid Ticks ◽  
Spotted Fever Group ◽  
Spotted Fever Group Rickettsiae

Spotted fever group rickettsiae in ixodid ticks in Oromia, Ethiopia

2015 ◽  
Vol 6 (1) ◽  
pp. 8-15 ◽  
Author(s):  
Bersissa Kumsa ◽  
Cristina Socolovschi ◽  
Didier Raoult ◽  
Philippe Parola
Keyword(s):  
Spotted Fever ◽  
Ixodid Ticks ◽  
Spotted Fever Group ◽  
Spotted Fever Group Rickettsiae

scholarly journals Isolation of spotted fever group rickettsiae from Ixodes persulcatus ticks in the central part of Hokkaido, Japan

2000 ◽  
Vol 51 (1) ◽  
pp. 55-58 ◽  
Author(s):  
Hiromi FUJITA ◽  
Nobuhiro TAKADA ◽  
Emiko ISOGAI ◽  
Yuriko WATANABE ◽  
Takuya ITO
Keyword(s):  
Spotted Fever ◽  
Ixodes Persulcatus ◽  
Spotted Fever Group ◽  
Spotted Fever Group Rickettsiae

scholarly journals Quantitative Analyses of Variations in the Injury of Endothelial Cells Elicited by 11 Isolates of Rickettsia rickettsii

2001 ◽  
Vol 8 (4) ◽  
pp. 788-796 ◽  
Author(s):  
Marina E. Eremeeva ◽  
Gregory A. Dasch ◽  
David J. Silverman
Keyword(s):  
North Carolina ◽  
Endothelial Cells ◽  
Reduced Glutathione ◽  
Spotted Fever ◽  
Ixodid Ticks ◽  
Spotted Fever Group ◽  
Cellular Injury ◽  
Rickettsia Rickettsii ◽  
Cytopathic Effects ◽  
Group I

ABSTRACT Eleven isolates of spotted fever group rickettsiae from the blood of patients or ixodid ticks from North and South America were characterized. All isolates were identified as Rickettsia rickettsii using restriction fragment length polymorphism analysis of a 532-bp rOmpA gene fragment obtained by PCR. The ability of the R. rickettsii isolates to elicit cytopathic effects and parameters of oxidative injury were examined in cultured human EA.hy 926 endothelial cells. Cytopathic effects were determined by direct observation of infected cultures, by measuring the release of cytoplasmic lactate dehydrogenase (LDH), and by determination of intracellular pools of peroxide and reduced glutathione. Four biotypes of R. rickettsii were defined. Group I included two highly cytopathic isolates from Montana, Bitterroot and Sheila Smith, and three isolates from Maryland, North Carolina, and Brazil. These isolates rapidly damaged cells, released large amounts of cytoplasmic LDH, caused accumulation of intracellular peroxide, and depleted intracellular pools of reduced glutathione. Group II contained three isolates, two from Montana, Hlp#2 and Lost Horse Canyon, and an isolate from Colombia, which were similar to group I but caused either lower responses in LDH release or smaller changes in intracellular peroxide levels. The group III isolates, Sawtooth from Montana and 84JG from North Carolina, caused lower cellular injury by all measures. Group IV isolate Price T from Montana was the least cytopathic and caused minimal alterations of all parameters measured. Understanding the molecular basis for the varied cellular injury caused by different isolates of R. rickettsii may contribute to improved treatment of Rocky Mountain spotted fever and to the rapid identification of those isolates which are more likely to cause fulminant disease.

scholarly journals Seroepidemiologic Study of Human Infections with Spotted Fever Group Rickettsiae in North Carolina

2014 ◽  
Vol 52 (11) ◽  
pp. 3960-3966 ◽  
Author(s):  
M. F. Vaughn ◽  
J. Delisle ◽  
J. Johnson ◽  
G. Daves ◽  
C. Williams ◽  
...  
Keyword(s):  
North Carolina ◽  
Spotted Fever ◽  
Spotted Fever Group ◽  
Spotted Fever Group Rickettsiae ◽  
Human Infections ◽  
Seroepidemiologic Study
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