scholarly journals Magnesium depletion extends fission yeast lifespan via general amino acid control activation

2021 ◽  
Vol 10 (2) ◽  
Author(s):  
Hokuto Ohtsuka ◽  
Mikuto Kobayashi ◽  
Takafumi Shimasaki ◽  
Teppei Sato ◽  
Genki Akanuma ◽  
...  
Keyword(s):  
Amino Acid ◽  
Fission Yeast ◽  
General Amino Acid Control ◽  
Acid Control ◽  
Magnesium Depletion ◽  
Control Activation

scholarly journals Structure of yeast regulatory geneLEU3and evidence thatLEU3itself is under general amino acid control

1987 ◽  
Vol 15 (13) ◽  
pp. 5261-5273 ◽  
Author(s):  
Kemin Zhou ◽  
Paula R.G. Brisco ◽  
Ari E. Hinkkanen ◽  
Gunter B. Kohlhaw
Keyword(s):  
Amino Acid ◽  
General Amino Acid Control ◽  
Acid Control

scholarly journals Boron Stress Activates the General Amino Acid Control Mechanism and Inhibits Protein Synthesis

PLoS ONE ◽  
2011 ◽  
Vol 6 (11) ◽  
pp. e27772 ◽  
Author(s):  
Irem Uluisik ◽  
Alaattin Kaya ◽  
Dmitri E. Fomenko ◽  
Huseyin C. Karakaya ◽  
Bradley A. Carlson ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Control Mechanism ◽  
General Amino Acid Control ◽  
Acid Control

scholarly journals Integration of General Amino Acid Control and Target of Rapamycin (TOR) Regulatory Pathways in Nitrogen Assimilation in Yeast

2010 ◽  
Vol 285 (22) ◽  
pp. 16893-16911 ◽  
Author(s):  
Kirk A. Staschke ◽  
Souvik Dey ◽  
John M. Zaborske ◽  
Lakshmi Reddy Palam ◽  
Jeanette N. McClintick ◽  
...  
Keyword(s):  
Amino Acid ◽  
Nitrogen Assimilation ◽  
Target Of Rapamycin ◽  
Regulatory Pathways ◽  
General Amino Acid Control ◽  
Acid Control

The yeast ILV2 gene is under general amino acid control

Genome ◽  
1988 ◽  
Vol 30 (6) ◽  
pp. 984-986 ◽  
Author(s):  
W. Xiao ◽  
G. H. Rank
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Key Words ◽  
Acetolactate Synthase ◽  
Amino Acid Starvation ◽  
Sulfometuron Methyl ◽  
General Amino Acid Control ◽  
Acid Control ◽  
High Level ◽  
Level Resistance

The yeast ILV2 gene encodes acetolactate synthase, the first enzyme in the biosynthesis of isoleucine and valine. Its multiple regulation has precluded the clear demonstration of whether ILV2 is under general amino acid control. Nonderepressible gcn4 strains were used as recipients for transformation with a YCp plasmid carrying GCN4. Parental gcn4 cells and their isogenic GCN4 transformants were evaluated for ALS derepression following induced amino acid starvation. GCN4 cells showed 1.5-to 1.7-fold derepression but no derepression was observed in isogenic control gcn4 strains. A similar depression of ILV2 mRNA was also observed. Genetic evidence for general amino acid control was the gcn4 suppression of high level resistance to sulfometuron methyl by the SMR1-410 allele of ILV2.Key words: Saccharomyces cerevisiae, ILV2 gene, general amino acid control, multiple regulators.

scholarly journals General amino acid control and specific arginine repression in Saccharomyces cerevisiae: physical study of the bifunctional regulatory region of the ARG3 gene.

1985 ◽  
Vol 5 (11) ◽  
pp. 3139-3148 ◽  
Author(s):  
M Crabeel ◽  
R Huygen ◽  
K Verschueren ◽  
F Messenguy ◽  
K Tinel ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Regulatory Region ◽  
Positive Control ◽  
Posttranscriptional Control ◽  
General Amino Acid Control ◽  
Acid Control ◽  
Physical Study ◽  
Specific Regulation ◽  
General Regulation

To characterize further the regulatory mechanism modulating the expression of the Saccharomyces cerevisiae ARG3 gene, i.e., the specific repression by arginine and the general amino acid control, we analyzed by deletion the region upstream of that gene, determined the nucleotide sequence of operator-constitutive-like mutations affecting the specific regulation, and examined the behavior of an ARG3-galK fusion engineered at the initiating codon of ARG3. Similarly to what was observed in previous studies on the HIS3 and HIS4 genes, our data show that the general regulation acts as a positive control and that a sequence containing the nucleotide TGACTC, between positions -364 and -282 upstream of the transcription start, functions as a regulatory target site. This sequence contains the most proximal of the two TGACTC boxes identified in front of ARG3. While the general control appears to modulate transcription efficiency, the specific repression by arginine displays a posttranscriptional component (F. Messenguy and E. Dubois, Mol. Gen. Genet. 189:148-156, 1983). Our deletion and gene fusion analyses confirm that the specific and general controls operate independently of each other and assign the site responsible for arginine-specific repression to between positions -170 and +22. In keeping with this assignment, the two operator-constitutive-like mutations were localized at positions -80 and -46, respectively, and thus in a region which is not transcribed. We discuss a hypothesis accounting for the involvement of untranscribed DNA in a posttranscriptional control.

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