scholarly journals Recombinant expression of an l ‐amino acid oxidase from the fungus Hebeloma cylindrosporum in Pichia pastoris including fermentation

2020 ◽  
Vol 9 (10) ◽  
Author(s):  
Marc Christian Heß ◽  
Svenja Bloess ◽  
Joe Max Risse ◽  
Karl Friehs ◽  
Gabriele Fischer von Mollard
Keyword(s):  
Amino Acid ◽  
Pichia Pastoris ◽  
Recombinant Expression ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Hebeloma Cylindrosporum

scholarly journals High-level expression of Rhodotorula gracilis d-amino acid oxidase in Pichia pastoris

2010 ◽  
Vol 33 (3) ◽  
pp. 557-563 ◽  
Author(s):  
Sandra Abad ◽  
Jozef Nahalka ◽  
Margit Winkler ◽  
Gabriele Bergler ◽  
Robert Speight ◽  
...  
Keyword(s):  
Amino Acid ◽  
Pichia Pastoris ◽  
Acid Oxidase ◽  
High Level Expression ◽  
Amino Acid Oxidase ◽  
Rhodotorula Gracilis ◽  
High Level

scholarly journals l-Amino acid oxidase of the fungus Hebeloma cylindrosporum displays substrate preference towards glutamate

Microbiology ◽  
2012 ◽  
Vol 158 (1) ◽  
pp. 272-283 ◽  
Author(s):  
Jaro T. Nuutinen ◽  
Eeva Marttinen ◽  
Rabah Soliymani ◽  
Kristiina Hildén ◽  
Sari Timonen
Keyword(s):  
Amino Acid ◽  
Substrate Preference ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Hebeloma Cylindrosporum

High expression of Trigonopsis variabilis d-amino acid oxidase in Pichia pastoris

2002 ◽  
Vol 18 (4-6) ◽  
pp. 291-297 ◽  
Author(s):  
Jian Yu ◽  
Dong-Yang Li ◽  
Ying-Jiu Zhang ◽  
Sheng Yang ◽  
Ren-bao Li ◽  
...  
Keyword(s):  
Amino Acid ◽  
Pichia Pastoris ◽  
High Expression ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Trigonopsis Variabilis

Expression, purification, and immobilization of His-tagged d-amino acid oxidase of Trigonopsis variabilis in Pichia pastoris

2006 ◽  
Vol 70 (6) ◽  
pp. 683-689 ◽  
Author(s):  
Huabao Zheng ◽  
Xiaolan Wang ◽  
Jun Chen ◽  
Ke Zhu ◽  
Yuhua Zhao ◽  
...  
Keyword(s):  
Amino Acid ◽  
Pichia Pastoris ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Trigonopsis Variabilis

Improved ammonia production from soybean residues by cell surface-displayed l-amino acid oxidase on yeast

2020 ◽  
Vol 85 (4) ◽  
pp. 972-980
Author(s):  
Yukio Watanabe ◽  
Wataru Aoki ◽  
Mitsuyoshi Ueda
Keyword(s):  
Amino Acid ◽  
Cell Surface ◽  
Conversion Efficiency ◽  
Food Processing ◽  
Yeast Cells ◽  
Ammonia Assimilation ◽  
Acid Oxidase ◽  
Ammonia Production ◽  
Amino Acid Oxidase ◽  
Hebeloma Cylindrosporum

ABSTRACT Ammonia is critical for agricultural and chemical industries. The extracellular production of ammonia by yeast (Saccharomyces cerevisiae) using cell surface engineering can be efficient approach because yeast can avoid growth deficiencies caused by knockout of genes for ammonia assimilation. In this study, we produced ammonia outside the yeast cells by displaying an l-amino acid oxidase with a wide substrate specificity derived from Hebeloma cylindrosporum (HcLAAO) on yeast cell surfaces. The HcLAAO-displaying yeast successfully produced 12.6 m m ammonia from a mixture of 20 proteinogenic amino acids (the theoretical conversion efficiency was 63%). We also succeeded in producing ammonia from a food processing waste, soybean residues (okara) derived from tofu production. The conversion efficiency was 88.1%, a higher yield than reported in previous studies. Our study demonstrates that ammonia production outside of yeast cells is a promising strategy to utilize food processing wastes.

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