Surface-Modified Metallic Biomaterials in Contact with Blood and Endothelial Cells

2007 ◽  
Vol 253 (1) ◽  
pp. 122-127 ◽  
Author(s):  
Bogdan Walkowiak
Keyword(s):  
Endothelial Cells ◽  
Metallic Biomaterials ◽  
Surface Modified

Assembly of Gentamicin and Zn2+ Loaded Coatings on TiO2 Nanotubes to Synergistically Improve the Blood Compatibility, Endothelial Cell Growth and Antibacterial Activities

2020 ◽  
Author(s):  
Changjiang Pan ◽  
Ya Yang ◽  
Youdong Hu ◽  
Li Quan ◽  
Yanchun Wei ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Blood Compatibility ◽  
Antibacterial Properties ◽  
Functional Expression ◽  
Antibacterial Activities ◽  
Layer By Layer ◽  
Zinc Ions ◽  
Continuous Release ◽  
Albumin Adsorption ◽  
Surface Modified

Abstract Titanium and its alloys are widely used in blood contacting implantable and interventional medical devices; however, their biocompatibility is still facing great challenges. In this study, with the aim of improving the biocompatibility and antibacterial activities of titanium, TiO2 nanotubes with a diameter of about 30 nm were firstly prepared on the titanium surface by anodization, followed by the introduction of polyacrylic acid (PAA) and gentamicin (GS) on the nanotube surface by layer-by-layer method, and finally zinc ions were loaded into the surface to improve the bioactivities. The nanotubes have excellent hydrophilic properties and special nanotube-like structure, which can selectively promote the albumin adsorption and enhance the blood compatibility and promote the growth and functional expression of endothelial cells to a certain extent. After the introduction of PAA and GS, although the super-hydrophilicity cannot be achieved, the results of platelet adhesion, cGMP activity, hemolysis rate and partial thromboplastin time (APTT) showed that the blood compatibility was improved, and the blood compatibility was further enhanced after zinc ions loading on the surface. On the other hand, the surface modified materials showed good cytocompatibility to endothelial cells. The introduction of PAA and zinc ions not only promoted the adhesion and proliferation of endothelial cells, but also up-regulated expression of vascular endothelial growth factor (VEGF) and nitric oxide (NO). The slow and continuous release of GS and Zn2+ for more than 14 days, which can significantly improve the antibacterial properties of the materials. Therefore, the present study provides an effective method for the surface modification of titanium-based blood-contacting materials to simultaneously endow with good blood compatibility, endothelial growth behaviors and antibacterial properties.

Human Vascular Endothelial Cells in Primary Cell Culture for the Evaluation of Nanoparticle Bioadhesion

2006 ◽  
Vol 6 (9) ◽  
pp. 3303-3309 ◽  
Author(s):  
Christoph Löhbach ◽  
Dirk Neumann ◽  
Claus-Michael Lehr ◽  
Alf Lamprecht
Keyword(s):  
Drug Delivery ◽  
Cell Culture ◽  
Endothelial Cells ◽  
Covalent Binding ◽  
Particle Diameter ◽  
Fluorescence Emission ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Human Endothelial Cells ◽  
Surface Modified

Nanoparticles (NP) are employed in various therapeutic approaches for innovative drug delivery strategies. Among them, there is drug delivery to the brain and sustained release forms for intravenous drug delivery. In order to optimize drug carriers and to elucidate involved mechanisms such as bioadhesion and cellular uptake, NP were surface modified and analyzed for their interaction with human endothelial cells in cell culture. Fluorescently labeled NP of different diameters (50 to 1000 nm) were surface modified either by simple adsorption of chitosan or by covalent binding to the lectin ulex europaeus agglutinin and thereafter applied to human endothelial cells for different incubation periods. After incubation with NP the binding of NP was quantified directly by the fluorescence emission signals from the cell layers. In order to visualize the binding behaviour, NP were localized three-dimensionally in the cell layer by confocal laser scanning microscopy. Cell binding experiments in phosphate buffer were observed to be particle size dependent with the 50 nm NP showing the highest binding percentage over all experiments. Binding decreased with increasing particle diameter and shorter incubation interval. The adhesion was further enhanced by NP surface modifications in the order blank < chitosan < lectin. The presence of plasma proteins enhanced the adhesiveness of chitosan coated NP, while the binding of lectin coated NP was inhibited. Experiments at 4 °C indicated the involvement of an active process in the binding of NP to endothelial cells.

scholarly journals Massive targeting of liposomes, surface-modified with anionized albumins, to hepatic endothelial cells

1997 ◽  
Vol 94 (21) ◽  
pp. 11681-11685 ◽  
Author(s):  
J. A. A. M. Kamps ◽  
H. W. M. Morselt ◽  
P. J. Swart ◽  
D. K. F. Meijer ◽  
G. L. Scherphof
Keyword(s):  
Endothelial Cells ◽  
Surface Modified

Behaviour of human endothelial cells on surface modified NiTi alloy

Biomaterials ◽  
2005 ◽  
Vol 26 (26) ◽  
pp. 5359-5367 ◽  
Author(s):  
S PLANT ◽  
D GRANT ◽  
L LEACH
Keyword(s):  
Endothelial Cells ◽  
Niti Alloy ◽  
Human Endothelial Cells ◽  
Surface Modified

Endotoxin Alteration of the Mucopolysaccharide Blood Vessel Coating in Rats

1974 ◽  
Vol 32 ◽  
pp. 148-149
Author(s):  
D. E. Philpott ◽  
A. Takahashi
Keyword(s):  
Skeletal Muscle ◽  
Endothelial Cells ◽  
Salivary Gland ◽  
Carotid Artery ◽  
Basement Membrane ◽  
Coronary Vessels ◽  
Ruthenium Red ◽  
E Coli ◽  
Old Rats ◽  
The Brain

Two month, eight month and two year old rats were treated with 10 or 20 mg/kg of E. Coli endotoxin I. P. The eight month old rats proved most resistant to the endotoxin. During fixation the aorta, carotid artery, basil arartery of the brain, coronary vessels of the heart, inner surfaces of the heart chambers, heart and skeletal muscle, lung, liver, kidney, spleen, brain, retina, trachae, intestine, salivary gland, adrenal gland and gingiva were treated with ruthenium red or alcian blue to preserve the mucopolysaccharide (MPS) coating. Five, 8 and 24 hrs of endotoxin treatment produced increasingly marked capillary damage, disappearance of the MPS coating, edema, destruction of endothelial cells and damage to the basement membrane in the liver, kidney and lung.

Complex Vesicles, Microtubules, and Cytoplasmic Filaments in the Endothelial Cells of Normal Dog Aorta

1968 ◽  
Vol 26 ◽  
pp. 172-173
Author(s):  
C. N. Sun ◽  
J. J. Ghidoni
Keyword(s):  
Electron Microscopy ◽  
Endothelial Cells ◽  
Cross Sections ◽  
Functional Significance ◽  
Tubular Structures ◽  
Aldehyde Fixation ◽  
Cytoplasmic Filaments

Endothelial cells in longitudinal and cross sections of aortas from 3 randomly selected “normal” mongrel dogs were studied by electron microscopy. Segments of aorta were distended with cold cacodylate buffered 5% glutaraldehyde for 10 minutes prior to being cut into small, well oriented tissue blocks. After an additional 1-1/2 hour period in glutaraldehyde, the tissue blocks were well rinsed in buffer and post-fixed in OsO4. After dehydration they were embedded in a mixture of Maraglas, D.E.R. 732, and DDSA.Aldehyde fixation preserves the filamentous and tubular structures (300 Å and less) for adequate demonstration and study. The functional significance of filaments and microtubules has been recently discussed by Buckley and Porter; the precise roles of these cytoplasmic components remains problematic. Endothelial cells in canine aortas contained an abundance of both types of structures.

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