Processing of Three-Dimensional Laser Sintered Polyetheretherketone Composites and Testing of Osteoblast Proliferation in vitro

2007 ◽  
Vol 253 (1) ◽  
pp. 65-70 ◽  
Author(s):  
Dirk Pohle ◽  
Sabine Ponader ◽  
Thomas Rechtenwald ◽  
Michael Schmidt ◽  
Karl Andreas Schlegel ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Osteoblast Proliferation ◽  
Proliferation In Vitro

The Effects of Exercise Serum From Prepubertal Girls and Women on In Vitro Myoblast and Osteoblast Proliferation and Differentiation

2021 ◽  
Vol 33 (2) ◽  
pp. 82-89
Author(s):  
Yasmeen Mezil ◽  
Joyce Obeid ◽  
Inna Ushcatz ◽  
Sandeep Raha ◽  
Brian W. Timmons
Keyword(s):  
Moderate Intensity ◽  
Osteoblast Proliferation ◽  
Moderate Intensity Exercise ◽  
Acute Bout ◽  
Myotube Formation ◽  
Proliferation And Differentiation ◽  
Proliferation In Vitro ◽  
Prepubertal Girls ◽  
Time Point

Purpose: In girls and women, the authors studied the effects of an acute bout of low-impact, moderate-intensity exercise serum on myoblast and osteoblast proliferation in vitro. Methods: A total of 12 pre/early pubertal girls (8–10 y old) and 12 women (20–30 y old) cycled at 60% VO2max for 1 hour followed by 1-hour recovery. Blood samples were collected at rest, mid-exercise, end of exercise, mid-recovery, and end of recovery. C2C12 myoblasts and MC3T3E1 osteoblasts were incubated with serum from each time point for 1 hour, then monitored for 24 hours (myoblasts) or 36 hours (osteoblasts) to examine proliferation. Cells were also monitored for 6 days (myoblasts) to examine myotube formation and 21 days (osteoblasts) to examine mineralization. Results: Exercise did not affect myoblast or osteoblast proliferation. Girls exhibited lower cell proliferation relative to women at end of exercise (osteoblasts, P = .041; myoblasts, P = .029) and mid-recovery (osteoblasts, P = .010). Mineralization was lower at end of recovery relative to rest (P = .014) in both girls and women. Myotube formation was not affected by exercise or group. Conclusion: The systemic environment following one acute bout of low-impact moderate-intensity exercise in girls and women does not elicit osteoblast or myoblast activity in vitro. Differences in myoblast and osteoblast proliferation between girls and women may be influenced by maturation.

Experimental Study on the Osteoinduction of Calcium Phosphate Biomaterials In Vivo and the Capability of Supporting Osteoblast Proliferation In Vitro

2005 ◽  
Vol 288-289 ◽  
pp. 281-286 ◽  
Author(s):  
C.Y. Bao ◽  
P. Li ◽  
Yan Fei Tan ◽  
Y. Cao ◽  
Xiao Yan Lin ◽  
...  
Keyword(s):  
Experimental Study ◽  
Surface Modification ◽  
Calcium Phosphate ◽  
Treated Group ◽  
Osteoblast Proliferation ◽  
Thin Slices ◽  
Proliferation In Vitro ◽  
Ceramic Cylinder

The purpose of this study was to evaluate the response of osteoblasts to calcium phosphate with different surface modification, and to evaluate the osteoinductive capabilities of these biomaterials. 60HA/40α-TCP ceramics sintered at 1250oC was applied in this study. A ceramic cylinder with F5mm×8mm and slice with F10mm×1mm were prepared respectively. One third of the ceramics was used to form bone-like apatite (BLA), and the surface of another one third was modified with collagen. Osteoblasts (1×106/ml) were co-cultured with the three kinds of thin slices for 12h, 24h and 48h. SEM observation was applied to evaluate whether the surface modification and BLA formation could affect the attachment and proliferation of osteoblast in vitro. The three kinds of cylinder samples were implanted in dog muscle to evaluate their differences in osteoinduction. Cells grew in multi-layers and attached to the surface and proliferated well in the collagen and HA/TCP group. In the untreated and BLA precipitated groups, cells did not attach to the surface well. Osteoinduction was good in the BLA precipitated group and the amount of bone formed was higher; in the untreated group and collagen-treated group, no bone formation was observed in the tested period. This result indicated that the scaffold used in cell-materials composites in vitro and that in osteoinductive material based tissue engineering in vivo was not same.

scholarly journals Determination of biophysical parameters for enhancement of human osteoblast proliferation by mechanical vibration in the infrasonic frequency range

2018 ◽  
Author(s):  
Rosenberg Nahum ◽  
Halevi Politch Jacob ◽  
Rosenberg Orit ◽  
Abramovich Haim
Keyword(s):  
Mechanical Stimulation ◽  
Culture Media ◽  
Mechanical Vibration ◽  
Mechanical Parameters ◽  
Human Osteoblast ◽  
Osteoblast Proliferation ◽  
Biophysical Parameters ◽  
Proliferation In Vitro ◽  
Stimulation Of

AbstractExperimental methods for studying an enhancement of osteoblast proliferation in vitro provide tools for the research of biochemical processes involved in bone turnover in vivo. Some of the current methods used for this purpose are based on the ability of the osteoblasts to enhance proliferation by mechanical stimulation. We describe an experimental approach of biomechanical stimulation of cultured human osteoblast-like cells by vibration. This method is based on the specially designed controlled vibration setup that consists of an electric actuator, with horizontally mounted well plate containing cell cultures. Previously this method found to be effective to enhance cell proliferation, but the exact mechanical parameters of effective vibration were elusive. The current low friction system for mechanical stimulation of osteoblast-like cells in vitro provides recording of narrow range mechanical parameters in the infrasonic spectrum.We exposed human osteoblast-like cells in explant monolayer culture to mechanical vibration in the 10-70Hz range of frequencies and found that 50-70 Hz of vibration frequency is optimal for inducing osteoblast proliferation that was deduced from interrelation between unchanged cell number in culture samples with significant decrease in cell death rate (decreased LDH activity in culture media, p<.05) and with parallel decrease of their maturation level (p<.01).In this report we determined the optimal mechanical parameters and excitation protocol for induction of osteoblast proliferation in vitro by using a tunable and versatile mechanical platform, which can be used in the research of cell mechanotransduction.

scholarly journals Optimal parameters for the enhancement of human osteoblast-like cell proliferation in vitro via shear stress induced by high-frequency mechanical vibration

2021 ◽  
Vol 3 (3) ◽  
pp. 204-211
Author(s):  
Nahum Rosenberg ◽  
◽  
Orit Rosenberg ◽  
Jacob Halevi Politch ◽  
Haim Abramovich ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Death ◽  
Monolayer Culture ◽  
Mechanical Vibration ◽  
Mechanical Parameters ◽  
Human Osteoblast ◽  
Osteoblast Proliferation ◽  
Static Conditions ◽  
Proliferation In Vitro

Introduction: Biomechanical stimulation of cultured human osteoblast-like cells, which is based on controlled mechanical vibration, has been previously indicated, but the exact mechanical parameters that are effective for cells' proliferation enhancement are still elusive due to the lack of direct data recordings from the stimulated cells in culture. Therefore, we developed a low friction tunable system that enables recording of a narrow range of mechanical parameters, above the infrasonic spectrum, that applied uniformly to human osteoblast-like cells in monolayer culture, aiming to identify a range of mechanical parameters that are effective to enhance osteoblast proliferation in vitro. Methods: Human osteoblast-like cells in explant monolayer culture samples were exposed to mechanical vibration in the 10-70Hz range of frequencies for two minutes, in four 24 hours intervals. Cell numbers in culture, cellular alkaline phosphatase activity (a marker of cell maturation), and lactate dehydrogenase activity in culture media (representing cell death) were measured after the mechanical stimulation protocol application and compared statistically to the control cell cultures kept in static conditions. The cell proliferation was deduced from cell number in culture and cell death measurements. Results: We found that 50-70 Hz of vibration frequency protocol (10-30 μm of maximal displacement amplitude, 0.03g of peak-to-peak acceleration) is optimal for enhancing cells' proliferation(p<0.05), with a parallel decrease of their maturation (p<0.01). Discussion: We detected the optimal mechanical parameters of excitation protocol for induction of osteoblast proliferation in vitro by a mechanical platform, which can be used as a standardized method in the research of mechanotransduction in human osteoblast.

The Fragments of Bovine High Molecular Weight Kininogen Promote Osteoblast Proliferation In Vitro

2006 ◽  
Vol 140 (6) ◽  
pp. 825-830 ◽  
Author(s):  
Jun-ichi Yamamura ◽  
Yoshikazu Morita ◽  
Yukihiro Takada ◽  
Hiroshi Kawakami
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Osteoblast Proliferation ◽  
High Molecular Weight Kininogen ◽  
Proliferation In Vitro

scholarly journals Pregnancy-Associated Plasma Protein-A Increases Osteoblast Proliferation in Vitro and Bone Formation in Vivo

Endocrinology ◽  
2006 ◽  
Vol 147 (12) ◽  
pp. 5653-5661 ◽  
Author(s):  
Xuezhong Qin ◽  
Jon E. Wergedal ◽  
Mark Rehage ◽  
Kiet Tran ◽  
Jacqueline Newton ◽  
...  
Keyword(s):  
Bone Formation ◽  
Transgenic Mice ◽  
Plasma Protein ◽  
Type I ◽  
Osteoblast Proliferation ◽  
Bone Formation Rate ◽  
Igf I ◽  
Proliferation In Vitro

Pregnancy-associated plasma protein (PAPP)-A, a protease for IGF binding protein (IGFBP)-2, -4, and -5, may enhance IGF action by increasing its bioavailability. Here we have determined the role and mechanism of action of PAPP-A in the regulation of osteoblast proliferation in vitro and bone metabolism in vivo. Recombinant PAPP-A (100 ng/ml) significantly increased osteoblast proliferation and free IGF-I concentration. These effects were abolished by noncleavable IGFBP-4, suggesting that PAPP-A promotes osteoblast proliferation by increasing IGF bioavailability. To determine whether PAPP-A exerts an anabolic effect on bone in vivo, we developed transgenic mice that overexpress PAPP-A in osteoblasts using the 2.3-kb rat type I collagen promoter. Consistent with the increase in IGFBP-4 proteolysis, free IGF-I concentration was significantly increased in the conditioned medium of cultured osteoblasts derived from transgenic mice compared with the wild-type littermates. Calvarial bone thickness, bone marrow cavity, and skull bone mineral density were significantly increased in transgenic mice. Bone size-related parameters in femur and tibia such as total bone area and periosteal circumference as determined by peripheral quantitated computed tomography and histological analysis were significantly increased in transgenic mice. Bone formation rate and osteoid surface were increased by more than 2-fold, whereas bone resorbing surface was unaffected. These anabolic effects were sustained with aging. These findings provide strong evidence that PAPP-A acts as a potent anabolic factor in the regulation of bone formation. Thus, enhancing IGF bioavailability by PAPP-A can be a powerful strategy in the treatment of certain metabolic diseases such as osteoporosis.

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