Alteration of Lipid Membrane Rigidity by Cholesterol and Its Metabolic Precursors

2005 ◽  
Vol 219 (1) ◽  
pp. 39-50 ◽  
Author(s):  
Horia I. Petrache ◽  
Daniel Harries ◽  
V. Adrian Parsegian
Keyword(s):  
Lipid Membrane ◽  
Membrane Rigidity ◽  
Metabolic Precursors

scholarly journals Polyglutamine aggregates impair lipid membrane integrity and enhance lipid membrane rigidity

2016 ◽  
Vol 1858 (4) ◽  
pp. 661-670 ◽  
Author(s):  
Chian Sing Ho ◽  
Nawal K. Khadka ◽  
Fengyu She ◽  
Jianfeng Cai ◽  
Jianjun Pan
Keyword(s):  
Lipid Membrane ◽  
Membrane Integrity ◽  
Membrane Rigidity

scholarly journals Influence of Lipid Membrane Rigidity on Properties of Supporting Polymer

2011 ◽  
Vol 101 (1) ◽  
pp. 128-133 ◽  
Author(s):  
Michael S. Jablin ◽  
Manish Dubey ◽  
Mikhail Zhernenkov ◽  
Ryan Toomey ◽  
Jarosław Majewski
Keyword(s):  
Lipid Membrane ◽  
Membrane Rigidity

scholarly journals Stiffness of fluid and gel phase lipid nanovesicles: weighting the contributions of membrane bending modulus and luminal pressurization

2021 ◽  
Author(s):  
Andrea Ridolfi ◽  
Lucrezia Caselli ◽  
Matteo Baldoni ◽  
Costanza Montis ◽  
Francesco Mercuri ◽  
...  
Keyword(s):  
Mechanical Response ◽  
Lipid Membrane ◽  
Dominant Role ◽  
Fluid Phase ◽  
Lipid Vesicles ◽  
Unified Model ◽  
Coarse Grained ◽  
Bending Modulus ◽  
Membrane Rigidity ◽  
Gel Phase

The mechanical properties of biogenic membranous compartments are thought to be relevant in numerous biological processes; however, their quantitative measurement remains challenging for most of the already available Force Spectroscopy (FS)-based techniques. In particular, the debate on the mechanics of lipid nanovesicles and on the interpretation of their mechanical response to an applied force is still open. This is mostly due to the current lack of a unified model being able to describe the mechanical response of gel and fluid phase lipid vesicles and to disentangle the contributions of membrane rigidity and luminal pressure. In this framework, we herein propose a simple model in which the contributions of membrane rigidity and luminal pressure to the overall vesicle stiffness are described as a series of springs; this approach allows estimating the two contributions for both gel and fluid phase liposomes. Atomic Force Microscopy-based FS (AFM-FS), performed on both vesicles and Supported Lipid Bilayers (SLBs), is exploited for obtaining all the parameters involved in the model. Moreover, the use of coarse-grained full-scale molecular dynamics simulations allowed for better understanding the differences in the mechanical responses of gel and fluid phase bilayers and supported the experimental findings. Results suggest that the pressure contribution is similar among all the probed vesicle types; however, it plays a dominant role in the mechanical response of lipid nanovesicles presenting a fluid phase membrane, while its contribution becomes comparable to the one of membrane rigidity in nanovesicles with a gel phase lipid membrane. The herein presented results offer a simple way to quantify two of the most important parameters in vesicular nanomechanics, and as such represent a first step towards a currently unavailable, unified model for the mechanical response of gel and fluid phase lipid nanovesicles.

scholarly journals The Vesicle Trafficking Protein Sar1 Lowers Lipid Membrane Rigidity

2010 ◽  
Vol 99 (5) ◽  
pp. 1539-1545 ◽  
Author(s):  
Edward I. Settles ◽  
Andrew F. Loftus ◽  
Alesia N. McKeown ◽  
Raghuveer Parthasarathy
Keyword(s):  
Lipid Membrane ◽  
Vesicle Trafficking ◽  
Membrane Rigidity

scholarly journals Scaling of lipid membrane rigidity with domain area fraction

Soft Matter ◽  
2019 ◽  
Vol 15 (13) ◽  
pp. 2762-2767 ◽  
Author(s):  
Elizabeth G. Kelley ◽  
Paul D. Butler ◽  
Michihiro Nagao
Keyword(s):  
Lipid Membrane ◽  
Area Fraction ◽  
Membrane Rigidity

The effective rigidity of phase separated membrane scales according to theory with no adjustable parameters.

scholarly journals Modification of Lipid Membrane Rigidity by the Yeast and Human Sar1 Vesicle Trafficking Proteins

2012 ◽  
Vol 102 (3) ◽  
pp. 106a
Author(s):  
Andrew F. Loftus ◽  
Vivian Hsieh ◽  
Raghuveer Parthasarathy
Keyword(s):  
Lipid Membrane ◽  
Vesicle Trafficking ◽  
Membrane Rigidity

Chapter 2b: The molecular antigenic structure of the TBEV

2020 ◽  
Keyword(s):  
Conformational Changes ◽  
Neutralizing Antibodies ◽  
Lipid Membrane ◽  
Cell Entry ◽  
Antigenic Structure ◽  
E Proteins ◽  
Amino Acid Sequence Level ◽  
Endosomal Membrane ◽  
E Protein ◽  
Golgi Network

TBEV-particles are assembled in an immature, noninfectious form in the endoplasmic reticulum by the envelopment of the viral core (containing the viral RNA) by a lipid membrane associated with two viral proteins, prM and E. Immature particles are transported through the cellular exocytic pathway and conformational changes induced by acidic pH in the trans-Golgi network allow the proteolytic cleavage of prM by furin, a cellular protease, resulting in the release of mature and infectious TBE-virions. The E protein controls cell entry by mediating attachment to as yet ill-defined receptors as well as by low-pH-triggered fusion of the viral and endosomal membrane after uptake by receptor-mediated endocytosis. Because of its key functions in cell entry, the E protein is the primary target of virus neutralizing antibodies, which inhibit these functions by different mechanisms. Although all flavivirus E proteins have a similar overall structure, divergence at the amino acid sequence level is up to 60 percent (e.g. between TBE and dengue viruses), and therefore cross-neutralization as well as (some degree of) cross-protection are limited to relatively closely related flaviviruses, such as those constituting the tick-borne encephalitis serocomplex.

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