Persistence Length of Cartilage Aggrecan Macromolecules Measured via Atomic Force Microscopy

2004 ◽  
Vol 214 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Laurel Ng ◽  
Alan J. Grodzinsky ◽  
John Sandy ◽  
Anna Plaas ◽  
Christine Ortiz
Keyword(s):  
Atomic Force Microscopy ◽  
Persistence Length ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Atomic force microscopy of the morphology and mechanical behaviour of barnacle cyprid footprint proteins at the nanoscale

2009 ◽  
Vol 7 (43) ◽  
pp. 285-296 ◽  
Author(s):  
In Yee Phang ◽  
Nick Aldred ◽  
Xing Yi Ling ◽  
Jurriaan Huskens ◽  
Anthony S. Clare ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Sea Water ◽  
Persistence Length ◽  
Force Microscopy ◽  
Bond Model ◽  
Atomic Force ◽  
Extension Force ◽  
Force Curves ◽  
High Elongation ◽  
Glass Surfaces

Barnacles are a major biofouler of man-made underwater structures. Prior to settlement, cypris larvae explore surfaces by reversible attachment effected by a ‘temporary adhesive’. During this exploratory behaviour, cyprids deposit proteinaceous ‘footprints’ of a putatively adhesive material. In this study, footprints deposited by Balanus amphitrite cyprids were probed by atomic force microscopy (AFM) in artificial sea water (ASW) on silane-modified glass surfaces. AFM images obtained in air yielded better resolution than in ASW and revealed the fibrillar nature of the secretion, suggesting that the deposits were composed of single proteinaceous nanofibrils, or bundles of fibrils. The force curves generated in pull-off force experiments in sea water consisted of regions of gradually increasing force, separated by sharp drops in extension force manifesting a characteristic saw-tooth appearance. Following the relaxation of fibrils stretched to high strains, force–distance curves in reverse stretching experiments could be described by the entropic elasticity model of a polymer chain. When subjected to relaxation exceeding 500 ms, extended footprint proteins refolded, and again showed saw-tooth unfolding peaks in subsequent force cycles. Observed rupture and hysteresis behaviour were explained by the ‘sacrificial bond’ model. Longer durations of relaxation (>5 s) allowed more sacrificial bond reformation and contributed to enhanced energy dissipation (higher toughness). The persistence length for the protein chains ( L P ) was obtained. At high elongation, following repeated stretching up to increasing upper strain limits, footprint proteins detached at total stretched length of 10 µm.

Persistence Length of Cylindrical Brush Molecules Measured by Atomic Force Microscopy

2006 ◽  
Vol 39 (6) ◽  
pp. 2219-2224 ◽  
Author(s):  
Nikhil Gunari ◽  
Manfred Schmidt ◽  
Andreas Janshoff
Keyword(s):  
Atomic Force Microscopy ◽  
Persistence Length ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Accurate nanoscale flexibility measurement of DNA and DNA–protein complexes by atomic force microscopy in liquid

Nanoscale ◽  
2017 ◽  
Vol 9 (31) ◽  
pp. 11327-11337 ◽  
Author(s):  
Divakaran Murugesapillai ◽  
Serge Bouaziz ◽  
L. James Maher ◽  
Nathan E. Israeloff ◽  
Craig E. Cameron ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Optical Tweezers ◽  
Protein Complexes ◽  
Persistence Length ◽  
Three Dimensional ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Imaging ◽  
Flexibility Measurement ◽  
Length Measurements

We obtain accurate three-dimensional persistence length measurements for DNA and DNA–protein complexes using liquid AFM imaging, validated by optical tweezers.

Effect of YOYO-1 on the mechanical properties of DNA

Soft Matter ◽  
2014 ◽  
Vol 10 (48) ◽  
pp. 9721-9728 ◽  
Author(s):  
Binu Kundukad ◽  
Jie Yan ◽  
Patrick S. Doyle
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Persistence Length ◽  
Contour Length ◽  
Force Microscopy ◽  
Atomic Force

Atomic force microscopy studies show that binding of YOYO-1 to DNA increases the contour length of DNA without affecting the persistence length due to the underwinding of DNA.

Contour and persistence length of Corynebacterium diphtheriae pili by atomic force microscopy

2012 ◽  
Vol 41 (6) ◽  
pp. 561-570 ◽  
Author(s):  
Johannes Rheinlaender ◽  
Anna Gräbner ◽  
Lisa Ott ◽  
Andreas Burkovski ◽  
Tilman E. Schäffer
Keyword(s):  
Atomic Force Microscopy ◽  
Persistence Length ◽  
Corynebacterium Diphtheriae ◽  
Force Microscopy ◽  
Atomic Force
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