Mass spectrometry-based plant metabolomics: Metabolite responses to abiotic stress

2015 ◽  
Vol 35 (5) ◽  
pp. 620-649 ◽  
Author(s):  
Tiago F. Jorge ◽  
João A. Rodrigues ◽  
Camila Caldana ◽  
Romy Schmidt ◽  
Joost T. van Dongen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Abiotic Stress ◽  
Plant Metabolomics

scholarly journals Metabolomics: A Way Forward for Crop Improvement

Metabolites ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 303 ◽  
Author(s):  
Ali Razzaq ◽  
Bushra Sadia ◽  
Ali Raza ◽  
Muhammad Khalid Hameed ◽  
Fozia Saleem
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Crop Improvement ◽  
Gas Chromatography Mass Spectrometry ◽  
Resonance Spectroscopy ◽  
Biotic And Abiotic Stress ◽  
Plant Metabolomics

Metabolomics is an emerging branch of “omics” and it involves identification and quantification of metabolites and chemical footprints of cellular regulatory processes in different biological species. The metabolome is the total metabolite pool in an organism, which can be measured to characterize genetic or environmental variations. Metabolomics plays a significant role in exploring environment–gene interactions, mutant characterization, phenotyping, identification of biomarkers, and drug discovery. Metabolomics is a promising approach to decipher various metabolic networks that are linked with biotic and abiotic stress tolerance in plants. In this context, metabolomics-assisted breeding enables efficient screening for yield and stress tolerance of crops at the metabolic level. Advanced metabolomics analytical tools, like non-destructive nuclear magnetic resonance spectroscopy (NMR), liquid chromatography mass-spectroscopy (LC-MS), gas chromatography-mass spectrometry (GC-MS), high performance liquid chromatography (HPLC), and direct flow injection (DFI) mass spectrometry, have sped up metabolic profiling. Presently, integrating metabolomics with post-genomics tools has enabled efficient dissection of genetic and phenotypic association in crop plants. This review provides insight into the state-of-the-art plant metabolomics tools for crop improvement. Here, we describe the workflow of plant metabolomics research focusing on the elucidation of biotic and abiotic stress tolerance mechanisms in plants. Furthermore, the potential of metabolomics-assisted breeding for crop improvement and its future applications in speed breeding are also discussed. Mention has also been made of possible bottlenecks and future prospects of plant metabolomics.

Mass spectrometry in plant metabolomics strategies: from analytical platforms to data acquisition and processing

2014 ◽  
Vol 31 (6) ◽  
pp. 784 ◽  
Author(s):  
Madeleine Ernst ◽  
Denise Brentan Silva ◽  
Ricardo Roberto Silva ◽  
Ricardo Z. N. Vêncio ◽  
Norberto Peporine Lopes
Keyword(s):  
Mass Spectrometry ◽  
Data Acquisition ◽  
Plant Metabolomics ◽  
Data Acquisition And Processing ◽  
Analytical Platforms

scholarly journals Proteomic profile of Piper tuberculatum (Piperaceae)

2017 ◽  
Vol 78 (1) ◽  
pp. 117-124 ◽  
Author(s):  
F. Cotinguiba ◽  
S. N. López ◽  
I. G. F. Budzinski ◽  
C. A. Labate ◽  
M. J. Kato ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Abiotic Stress ◽  
Secondary Metabolites ◽  
Defense Mechanism ◽  
Biological Activities ◽  
Proteomic Profile ◽  
Biotic And Abiotic Stress ◽  
Sds Page ◽  
Proteomic Study ◽  
Molecular Information

Abstract Piper tuberculatum (Piperaceae) is a species that accumulates especially amides as secondary metabolites and several biological activities was previously reported. In this article, we report a proteomic study of P. tuberculatum. Bidimensional electrophoresis (2D SDS-PAGE) and mass spectrometry (ESI-Q-TOF) were used in this study. Over a hundred spots and various peptides were identified in this species and the putative functions of these peptides related to defense mechanism as biotic and abiotic stress were assigned. The information presented extend the range of molecular information of P. tuberculatum.

scholarly journals Mass spectrometry as a quantitative tool in plant metabolomics

2016 ◽  
Vol 374 (2079) ◽  
pp. 20150370 ◽  
Author(s):  
Tiago F. Jorge ◽  
Ana T. Mata ◽  
Carla António
Keyword(s):  
Mass Spectrometry ◽  
Chemical Species ◽  
Chemical Properties ◽  
Research Field ◽  
Plant Metabolomics ◽  
Wide Range ◽  
Metabolite Pool ◽  
Development And Validation ◽  
Analytical Tools ◽  
Quantitative Tool

Metabolomics is a research field used to acquire comprehensive information on the composition of a metabolite pool to provide a functional screen of the cellular state. Studies of the plant metabolome include the analysis of a wide range of chemical species with very diverse physico-chemical properties, and therefore powerful analytical tools are required for the separation, characterization and quantification of this vast compound diversity present in plant matrices. In this review, challenges in the use of mass spectrometry (MS) as a quantitative tool in plant metabolomics experiments are discussed, and important criteria for the development and validation of MS-based analytical methods provided. This article is part of the themed issue ‘Quantitative mass spectrometry’.

scholarly journals Mass Spectrometry‐Based Untargeted Plant Metabolomics

2019 ◽  
Vol 4 (4) ◽  
Author(s):  
Leonardo Perez de Souza ◽  
Saleh Alseekh ◽  
Thomas Naake ◽  
Alisdair Fernie
Keyword(s):  
Mass Spectrometry ◽  
Plant Metabolomics

Proton Transfer Reaction Mass Spectrometry for Plant Metabolomics

2020 ◽  
Vol 25 (3) ◽  
pp. 313-314 ◽  
Author(s):  
Tomasz Majchrzak ◽  
Wojciech Wojnowski ◽  
Andrzej Wasik
Keyword(s):  
Mass Spectrometry ◽  
Proton Transfer ◽  
Transfer Reaction ◽  
Proton Transfer Reaction ◽  
Reaction Mass ◽  
Plant Metabolomics
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