Single Molecule Studies of a Ladder Type Conjugated Polymer: Vibronic Spectra, Line Widths, and Energy Transfer

2015 ◽  
Vol 36 (11) ◽  
pp. 1096-1102 ◽  
Author(s):  
Martin F. Zickler ◽  
Florian A. Feist ◽  
Josemon Jacob ◽  
Klaus Müllen ◽  
Thomas Basché
Keyword(s):  
Energy Transfer ◽  
Single Molecule ◽  
Conjugated Polymer ◽  
Vibronic Spectra ◽  
Single Molecule Studies ◽  
Line Widths

Single-Molecule Studies of Electronic Energy Transfer in a Diblock Conjugated Polymer

2005 ◽  
Vol 44 (38) ◽  
pp. 6207-6210 ◽  
Author(s):  
John K. Grey ◽  
Doo Young Kim ◽  
Young Jong Lee ◽  
Jose J. Gutierrez ◽  
Nam Luong ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Single Molecule ◽  
Conjugated Polymer ◽  
Electronic Energy ◽  
Electronic Energy Transfer ◽  
Single Molecule Studies

Single-Molecule Studies of Electronic Energy Transfer in a Diblock Conjugated Polymer

2005 ◽  
Vol 117 (38) ◽  
pp. 6363-6366 ◽  
Author(s):  
John K. Grey ◽  
Doo Young Kim ◽  
Young Jong Lee ◽  
Jose J. Gutierrez ◽  
Nam Luong ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Single Molecule ◽  
Conjugated Polymer ◽  
Electronic Energy ◽  
Electronic Energy Transfer ◽  
Single Molecule Studies

Monitoring the Structural Dynamics of U2 snRNA Via Single-Molecule Förster Resonance Energy Transfer

2018 ◽  
Author(s):  
Alexander Carl DeHaven
Keyword(s):  
Energy Transfer ◽  
Structural Dynamics ◽  
Single Molecule ◽  
Conformational Dynamics ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Förster Resonance Energy Transfer ◽  
U2 Snrna ◽  
Folding Dynamics ◽  
The Impact

This thesis contains four topic areas: a review of single-molecule microscropy methods and splicing, conformational dynamics of stem II of the U2 snRNA, the impact of post-transcriptional modifications on U2 snRNA folding dynamics, and preliminary findings on Mango aptamer folding dynamics.

scholarly journals Hopping and Flipping of RNA Polymerase on DNA during Recycling for Reinitiation after Intrinsic Termination in Bacterial Transcription

2021 ◽  
Vol 22 (5) ◽  
pp. 2398
Author(s):  
Wooyoung Kang ◽  
Seungha Hwang ◽  
Jin Young Kang ◽  
Changwon Kang ◽  
Sungchul Hohng
Keyword(s):  
Single Molecule ◽  
Transcription Initiation ◽  
Molecular Mechanisms ◽  
Facilitated Diffusion ◽  
One Dimensional ◽  
Bacterial Transcription ◽  
Fluorescence Measurements ◽  
Dimensional Diffusion ◽  
Single Molecule Studies ◽  
Hopping Diffusion

Two different molecular mechanisms, sliding and hopping, are employed by DNA-binding proteins for their one-dimensional facilitated diffusion on nonspecific DNA regions until reaching their specific target sequences. While it has been controversial whether RNA polymerases (RNAPs) use one-dimensional diffusion in targeting their promoters for transcription initiation, two recent single-molecule studies discovered that post-terminational RNAPs use one-dimensional diffusion for their reinitiation on the same DNA molecules. Escherichia coli RNAP, after synthesizing and releasing product RNA at intrinsic termination, mostly remains bound on DNA and diffuses in both forward and backward directions for recycling, which facilitates reinitiation on nearby promoters. However, it has remained unsolved which mechanism of one-dimensional diffusion is employed by recycling RNAP between termination and reinitiation. Single-molecule fluorescence measurements in this study reveal that post-terminational RNAPs undergo hopping diffusion during recycling on DNA, as their one-dimensional diffusion coefficients increase with rising salt concentrations. We additionally find that reinitiation can occur on promoters positioned in sense and antisense orientations with comparable efficiencies, so reinitiation efficiency depends primarily on distance rather than direction of recycling diffusion. This additional finding confirms that orientation change or flipping of RNAP with respect to DNA efficiently occurs as expected from hopping diffusion.

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