Injectable Biodegradable Poly(ethylene glycol)/RGD Peptide Hybrid Hydrogels for in vitro Chondrogenesis of Human Mesenchymal Stem Cells

2010 ◽  
Vol 31 (13) ◽  
pp. 1148-1154 ◽  
Author(s):  
Shao Qiong Liu ◽  
Quan Tian ◽  
Lei Wang ◽  
James L. Hedrick ◽  
James Hoi Po Hui ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethylene Glycol ◽  
Human Mesenchymal Stem Cells ◽  
Rgd Peptide ◽  
Poly Ethylene Glycol ◽  
Hybrid Hydrogels ◽  
Biodegradable Poly ◽  
Poly Ethylene

scholarly journals Osteogenic differentiation of human mesenchymal stem cells on poly(ethylene glycol)-variant biomaterials

2009 ◽  
Vol 91A (4) ◽  
pp. 975-984 ◽  
Author(s):  
Tonye Briggs ◽  
Matthew D. Treiser ◽  
Paul F. Holmes ◽  
Joachim Kohn ◽  
Prabhas V. Moghe ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethylene Glycol ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals Osteogenic potential of poly(ethylene glycol)-amorphous calcium phosphate composites on human mesenchymal stem cells

2020 ◽  
Vol 11 ◽  
pp. 204173142092684 ◽  
Author(s):  
Aman S Chahal ◽  
Manuel Schweikle ◽  
Aina-Mari Lian ◽  
Janne E Reseland ◽  
Håvard J Haugen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Ethylene Glycol ◽  
Osteogenic Differentiation ◽  
Amorphous Calcium Phosphate ◽  
Human Mesenchymal Stem Cells ◽  
Osteogenic Potential ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Synthetic hydrogel-amorphous calcium phosphate composites are promising candidates to substitute biologically sourced scaffolds for bone repair. While the hydrogel matrix serves as a template for stem cell colonisation, amorphous calcium phosphate s provide mechanical integrity with the potential to stimulate osteogenic differentiation. Here, we utilise composites of poly(ethylene glycol)-based hydrogels and differently stabilised amorphous calcium phosphate to investigate potential effects on attachment and osteogenic differentiation of human mesenchymal stem cells. We found that functionalisation with integrin binding motifs in the form of RGD tripeptide was necessary to allow adhesion of large numbers of cells in spread morphology. Slow dissolution of amorphous calcium phosphate mineral in the scaffolds over at least 21 days was observed, resulting in the release of calcium and zinc ions into the cell culture medium. While we qualitatively observed an increasingly mineralised extracellular matrix along with calcium deposition in the presence of amorphous calcium phosphate-loaded scaffolds, we did not observe significant changes in the expression of selected osteogenic markers.

Structural engineering to control density, conformation, and bioactivity of the poly(ethylene glycol)-grafted poly(urethane urea) scaffolds

2018 ◽  
Vol 34 (2) ◽  
pp. 209-223
Author(s):  
Shideh Shaneh ◽  
Fatemeh Shokrolahi ◽  
Parvin Shokrollahi ◽  
Hamid Yeganeh ◽  
Hossein Omidian
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethylene Glycol ◽  
Structural Engineering ◽  
Cell Attachment ◽  
Poly Ethylene Glycol ◽  
Salt Leaching ◽  
The Matrix ◽  
Diphenyl Tetrazolium Bromide ◽  
Poly Ethylene

Poly(urethane urea) scaffolds were fabricated through combined salt leaching and solvent casting methods. The scaffolds were then functionalized via aminolysis with poly(ethylene glycol) (PEG- g-PUU). To compare its bioactivity, gelatin was also grafted onto the aminolyzed poly(urethane urea) surface (Gel- g-PUU). Chemical changes at the surface were then monitored using quantitative/qualitative methods. Grafting with both gelatin and poly(ethylene glycol) remarkably enhanced the wettability of poly(urethane urea). Proliferation of human adipose–derived mesenchymal stem cells on poly(urethane urea) and the modified poly(urethane urea)s was evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay. The cell experiment results showed that both the modified poly(urethane urea)s enhanced the attachment and proliferation of human adipose–derived mesenchymal stem cells compared to pure poly(urethane urea). Based on previous reports, while a supportive role is observed at adequate poly(ethylene glycol) graft densities, cell adhesion and proliferation are inhibited at very high grafting densities. To correlate the cell data to poly(ethylene glycol) conformations, the surface tension was measured. Data on human adipose–derived mesenchymal stem cells’ attachment/proliferation and contact angle/surface free energy together showed that the grafting density of poly(ethylene glycol) was regulated by optimizing aminolysis conditions, careful selection of poly(ethylene glycol)’s molecular weight, and bulk properties of the matrix poly(urethane urea). As a result, surface overcrowding and brush conformation of the poly(ethylene glycol) chains were avoided, and human adipose–derived mesenchymal stem cell attachment and proliferation occurred on the PEG- g-PUU scaffold at a comparable level to the Gel- g-PUU.

scholarly journals Poly(3-hydroxybutyrate)/poly(ethylene glycol) scaffolds with different microstructure: the effect on growth of mesenchymal stem cells

3 Biotech ◽  
2018 ◽  
Vol 8 (8) ◽  
Author(s):  
A. P. Bonartsev ◽  
I. I. Zharkova ◽  
V. V. Voinova ◽  
E. S. Kuznetsova ◽  
V. A. Zhuikov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Effect On Growth
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