Degradation of Poly(methyl methacrylate) Model Compounds at Constant Elevated Temperature Studied via High Resolution Electrospray Ionization Mass Spectrometry (ESI-MS)

2007 ◽  
Vol 28 (16) ◽  
pp. 1593-1600 ◽  
Author(s):  
Francesca Bennet ◽  
Tara M. Lovestead ◽  
Philip J. Barker ◽  
Thomas P. Davis ◽  
Martina H. Stenzel ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Elevated Temperature ◽  
High Resolution ◽  
Methyl Methacrylate ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Model Compounds ◽  
Esi Ms

Degradation of poly(butyl methacrylate) model compounds studied via high-resolution electrospray ionization mass spectrometry

2010 ◽  
Vol 49 (4) ◽  
pp. 848-861 ◽  
Author(s):  
Alexander H. Soeriyadi ◽  
Francesca Bennet ◽  
Michael R. Whittaker ◽  
Philip J. Barker ◽  
Christopher Barner-Kowollik ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Ionization Mass Spectrometry ◽  
Butyl Methacrylate ◽  
Ionization Mass ◽  
Model Compounds

scholarly journals Analysis of Inositol Phosphate Metabolism by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry (CE-ESI-MS)

2020 ◽  
Author(s):  
Danye Qiu ◽  
Miranda S. Wilson ◽  
Verena B. Eisenbeis ◽  
Robert K. Harmel ◽  
Esther Riemer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Electrospray Ionization ◽  
Electrospray Ionization Mass Spectrometry ◽  
Inositol Phosphate ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Phosphate Metabolism ◽  
Esi Ms ◽  
Inositol Phosphate Metabolism

AbstractThe analysis of myo-inositol phosphates (InsPs) and myo-inositol pyrophosphates (PP-InsPs) is a daunting challenge due to the large number of possible isomers, the absence of a chromophore, the high charge density, the low abundance, and the instability of the esters and anhydrides. Given their importance in biology, an analytical approach to follow and understand this complex signaling hub is highly desirable. Here, capillary electrophoresis (CE) coupled to electrospray ionization mass spectrometry (ESI-MS) is implemented to analyze complex mixtures of InsPs and PP-InsPs with high sensitivity. Stable isotope labeled (SIL) internal standards allow for matrix-independent quantitative assignment. The method is validated in wild-type and knockout mammalian cell lines and in model organisms. SIL-CE-ESI-MS enables for the first time the accurate monitoring of InsPs and PP-InsPs arising from compartmentalized cellular synthesis pathways, by feeding cells with either [13C6]-myo-inositol or [13C6]-D-glucose. In doing so, we uncover that there must be unknown inositol synthesis pathways in mammals, highlighting the unique potential of this method to dissect inositol phosphate metabolism and signalling.

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