Gelation Rate Modulation of anα-Cyclodextrin and Poly(ethylene glycol)-Grafted Hyaluronic Acid Solution System by Inclusion Complexation of a Microphase-Separated Structure

2004 ◽  
Vol 25 (6) ◽  
pp. 739-742 ◽  
Author(s):  
Tsuyoshi Nakama ◽  
Tooru Ooya ◽  
Nobuhiko Yui
Keyword(s):  
Hyaluronic Acid ◽  
Ethylene Glycol ◽  
Acid Solution ◽  
Inclusion Complexation ◽  
Poly Ethylene Glycol ◽  
Solution System ◽  
Rate Modulation ◽  
Gelation Rate ◽  
Poly Ethylene

A bioactive hyaluronic acid–based hydrogel cross-linked by Diels–Alder reaction for promoting neurite outgrowth of PC12 cells

2017 ◽  
Vol 32 (4) ◽  
pp. 382-396 ◽  
Author(s):  
Dongming Xing ◽  
Lie Ma ◽  
Changyou Gao
Keyword(s):  
Hyaluronic Acid ◽  
Ethylene Glycol ◽  
Neurite Outgrowth ◽  
Pc12 Cells ◽  
Alder Reaction ◽  
Poly Ethylene Glycol ◽  
Substitution Degree ◽  
Poly Ethylene

In order to improve neurite outgrowth on the in situ formed hyaluronic acid–based hydrogel, furan and methacrylate groups were grafted on hyaluronic acid successively. Furthermore, a laminin-derived peptide CQAASIKVAV was covalently immobilized via the Michael addition. The furan- and peptide-modified hyaluronic acid was then cross-linked in situ by mixing with bismaleimide poly(ethylene glycol) at 37 °C to obtain a bioactive hyaluronic acid–based hydrogel. The hyaluronic acid derivatives were characterized by 1H NMR and Fourier transform infrared spectroscopy. The gelation, swelling, and mechanical property of the hydrogels were analyzed. The modulus of the hydrogel could be tuned by changing furan substitution degree, while the peptide concentration could be changed by the ratio of furan- and peptide-modified hyaluronic acid with hyaluronic acid–furan. In vitro culture of PC12 cells showed that the longest neurite outgrowth appeared on the hyaluronic acid–poly(ethylene glycol) hydrogel with the highest peptide content (the substitution degree of peptide in furan- and peptide-modified hyaluronic acid was 23 %) and a lower threshold modulus of 4.5 kPa. The furan and methacrylate-functionalized hyaluronic acid provides a versatile platform for diverse functionalization and can be used for modulation of other cell behaviors as well.

scholarly journals Hydrogels assembled by inclusion complexation of poly(ethylene glycol) with alpha-cyclodextrin

2009 ◽  
Vol 4 (5) ◽  
pp. 544-550 ◽  
Author(s):  
Jie Wang ◽  
Li Li ◽  
Yunying Zhu ◽  
Peng Liu ◽  
Xuhong Guo
Keyword(s):  
Ethylene Glycol ◽  
Inclusion Complexation ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene
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