In Situ Crosslinking of Highly Porous Chitosan Scaffolds for Bone Regeneration: Production Parameters and In Vitro Characterization

2017 ◽  
Vol 302 (10) ◽  
pp. 1700147 ◽  
Author(s):  
Benjamin Kruppke ◽  
Jana Farack ◽  
Freya Sommer ◽  
Simy Weil ◽  
Eliahu David Aflalo ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Production Parameters ◽  
In Vitro Characterization ◽  
Porous Chitosan ◽  
Chitosan Scaffolds ◽  
In Situ Crosslinking ◽  
Highly Porous

In vitro characterization of an injectable in situ forming composite system for bone reconstruction

2015 ◽  
Vol 119 ◽  
pp. 151-158 ◽  
Author(s):  
R. Dorati ◽  
C. Colonna ◽  
I. Genta ◽  
A. De Trizio ◽  
T. Modena ◽  
...  
Keyword(s):  
Composite System ◽  
Bone Reconstruction ◽  
In Situ Forming ◽  
In Vitro Characterization

scholarly journals Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Takeshi Ikeda ◽  
Kahori Ikeda ◽  
Kouhei Yamamoto ◽  
Hidetaka Ishizaki ◽  
Yuu Yoshizawa ◽  
...  
Keyword(s):  
Tensile Strength ◽  
Porous Scaffold ◽  
Chitosan Solution ◽  
Spongiform Encephalopathy ◽  
Porous Chitosan ◽  
Chitosan Scaffolds ◽  
Controlled Freezing ◽  
The Relationship ◽  
Releasing Time

Cells, growth factors, and scaffolds are the three main factors required to create a tissue-engineered construct. After the appearance of bovine spongiform encephalopathy (BSE), considerable attention has therefore been focused on nonbovine materials. In this study, we examined the properties of a chitosan porous scaffold. A porous chitosan sponge was prepared by the controlled freezing and lyophilization of different concentrations of chitosan solutions. The materials were examined by scanning electron microscopy, and the porosity, tensile strength, and basic fibroblast growth factor (bFGF) release profiles from chitosan sponge were examined in vitro. The morphology of the chitosan scaffolds presented a typical microporous structure, with the pore size ranging from 50 to 200 μm. The porosity of chitosan scaffolds with different concentrations was approximately 75–85%. A decreasing tendency for porosity was observed as the concentration of the chitosan increased. The relationship between the tensile properties and chitosan concentration indicated that the ultimate tensile strength for the sponge increased with a higher concentration. The in vitro bFGF release study showed that the higher the concentration of chitosan solution became, the longer the releasing time of the bFGF from the chitosan sponge was.

scholarly journals Development of a Mucoadhesive and In Situ Gelling Formulation Based on κ-Carrageenan for Application on Oral Mucosa and Esophagus Walls. I. A Functional In Vitro Characterization

Marine Drugs ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. 112 ◽  
Author(s):  
Barbara Vigani ◽  
Angela Faccendini ◽  
Silvia Rossi ◽  
Giuseppina Sandri ◽  
Maria Bonferoni ◽  
...  
Keyword(s):  
Oral Mucositis ◽  
Protective Action ◽  
Rheological Characterization ◽  
Low Viscosity ◽  
In Vitro Characterization ◽  
Biological Substrates ◽  
Clinically Significant ◽  
In Situ Gelling

Oral mucositis and esophagitis represent the most frequent and clinically significant complications of cytoreductive chemotherapy and radiotherapy, which severely compromise the patient quality of life. The local application of polymeric gels could protect the injured tissues, alleviating the most painful symptoms. The present work aims at developing in situ gelling formulations for the treatment of oral mucositis and esophagitis. To reach these targets, κ-carrageenan (κ-CG) was selected as a polymer having wound healing properties and able to gelify in the presence of saliva ions, while hydroxypropyl cellulose (HPC) was used to improve the mucoadhesive properties of the formulations. CaCl2 was identified as a salt able to enhance the interaction between κ-CG and saliva ions. Different salt and polymer concentrations were investigated in order to obtain a formulation having the following features: (i) low viscosity at room temperature to facilitate administration, (ii) marked elastic properties at 37 °C, functional to a protective action towards damaged tissues, and (iii) mucoadhesive properties. Prototypes characterized by different κ-CG, HPC, and CaCl2 concentrations were subjected to a thorough rheological characterization and to in vitro mucoadhesion and washability tests. The overall results pointed out the ability of the developed formulations to produce a gel able to interact with saliva ions and to adhere to the biological substrates.

Preparation and in vitro characterization of electrospun PVA scaffolds coated with bioactive glass for bone regeneration

2012 ◽  
Vol 100A (5) ◽  
pp. 1324-1334 ◽  
Author(s):  
Chunxia Gao ◽  
Qiang Gao ◽  
Yadong Li ◽  
Mohamed N. Rahaman ◽  
Akira Teramoto ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Bone Regeneration ◽  
In Vitro Characterization

Identification and in situ and in vitro characterization of secreted proteins produced by plant-parasitic nematodes

Parasitology ◽  
1996 ◽  
Vol 113 (6) ◽  
pp. 589-597 ◽  
Author(s):  
R. H. C. Curtis
Keyword(s):  
Parasitic Nematodes ◽  
Plant Parasitic Nematodes ◽  
Root Cells ◽  
Feeding Site ◽  
In Vitro Characterization ◽  
Plant Nematode ◽  
Plant Parasitic

SUMMARYSecretions of plant-parasitic nematodes which are released into plant tissue may play critical roles in plant-nematode interactions. The identification and characterization of these molecules are of fundamental importance and may help to facilitate the development of novel strategies to interfere with nematode infection of plants and thereby decrease nematode-induced damage to crops. An antibody-based approach was used to isolate molecules present on the nematode surface and in nematode secretions. Monoclonal antibodies (MAbs) were produced to secretions and to whole Heterodera avenue 2nd-stage juveniles; several of these MAbs recognized molecules present in nematode secretions produced in vitro. Three of these molecules have been partly characterized in H. avenae, Globodera rostochiensis, G. pallida and Meloidogyne incognita. A MAb reacting with the surfaces of these nematodes recognized antigens of different molecular weight in each of the species tested. This difference in antigenicity might be related to specific functions in these nematodes. Preliminary results show that this antibody also localized the antigen in root cells surrounding the feeding site induced by M. incognita in Arabidopsis thaliana.

Sign in / Sign up

Export Citation Format

Share Document