High‐Performance Sieving Electrophoresis for Single‐Nucleotide Polymorphisms with a Structuring Hydrogel Network

2019 ◽  
Vol 221 (2) ◽  
pp. 1900385
Author(s):  
Qingxiang You ◽  
Ping Wang ◽  
Dawei Zhang ◽  
Zhenqing Li ◽  
Yoshinori Yamaguchi
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
High Performance ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Hydrogel Network

Genotyping single nucleotide polymorphisms by primer extension and high performance liquid chromatography

1999 ◽  
Vol 104 (1) ◽  
pp. 89-93 ◽  
Author(s):  
Bastiaan Hoogendoorn ◽  
Michael J. Owen ◽  
Peter J. Oefner ◽  
Nigel Williams ◽  
Jehannine Austin ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Single Nucleotide Polymorphisms ◽  
High Performance ◽  
Primer Extension ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

scholarly journals Genotyping of BCL11A and HBS1L-MYB Single Nucleotide Polymorphisms in β-thalassemia/HbE and Homozygous HbE Subjects with Low and High Levels of HbF

2017 ◽  
Vol 15 (9) ◽  
pp. 627-636
Author(s):  
Watcharee PRASING ◽  
Chadia MEKKI ◽  
Patrinee TRAISATHIT ◽  
Serge PISSARD ◽  
Sakorn PORNPRASERT
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
High Performance ◽  
Hematological Parameters ◽  
Hematological Parameter ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Myb Gene ◽  
Genotype Frequencies ◽  
Hbf Level ◽  
Myb Genes

Whether multiple single nucleotide polymorphisms (SNPs) of BCL11A and HBS1L-MYB genes affect hemoglobin (Hb)F production and hematological parameter variation in β-thalassemia/HbE and homozygous HbE in Thai subjects with low and high HbF levels are still unclear. Three SNPs of BCL11A gene (rs1427407, rs10189857 and rs11886868) and 3 SNPs of HBS1L-MYB gene (rs4895441, rs9399137 and rs28384513) were analyzed in 45 β-thalassemia/HbE patients who had HbF levels lower and higher than 15 %, and in 50 homozygous HbE who had HbF levels lower and higher than 5 %. Their hematological parameters were measured using automated blood counter. The HbF level was analyzed using high performance liquid chromatography (HPLC). There were no statistical significant differences of allele and genotype frequencies of 3 SNPs in the BCL11A gene between the groups of β-thalassemia/HbE patients and homozygous HbE subjects with low and high HbF levels. Significant differences in the allele frequencies in HBS1L-MYB SNP rs4895441 (p = 0.041) and rs9399137 (p = 0.048) were observed in homozygous HbE subjects with HbF £ 5 % and > 5 %. Moreover, significant differences in MCV (p = 0.005) and trends towards significant differences in MCH (p = 0.057) and HbF levels (p = 0.051) were found in HBS1L-MYB SNP rs9399137 of homozygous HbE subjects. Therefore, the HBS11L-MYB SNPs especially rs9399137 had an effect on HbF production and the variation of hematological parameters in homozygous HbE subjects, but not in β-thalassemia/HbE patients.

Development of a simple method for the determination of single nucleotide polymorphisms

2010 ◽  
Vol 34 (8) ◽  
pp. S75-S75
Author(s):  
Weifeng Zhu ◽  
Zhuoqi Liu ◽  
Daya Luo ◽  
Xinyao Wu ◽  
Fusheng Wan
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Nucleotide Polymorphisms ◽  
Simple Method ◽  
Single Nucleotide
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