Determination of Intimate Composition of Theranostic Polyplexes Based on (Co)Polymers of Poly(vinyl benzyl trimethylammonium chloride)

2017 ◽  
Vol 219 (4) ◽  
pp. 1700428 ◽  
Author(s):  
Emi Haladjova ◽  
Grigoris Mountrichas ◽  
Stergios Pispas ◽  
Stanislav Rangelov
Keyword(s):  
Trimethylammonium Chloride ◽  
Vinyl Benzyl

scholarly journals Poly[oligo(ethylene glycol) methacrylate]-b-poly[(vinyl benzyl trimethylammonium chloride)] Based Multifunctional Hybrid Nanostructures Encapsulating Magnetic Nanoparticles and DNA

Polymers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1283
Author(s):  
Angeliki Chroni ◽  
Aleksander Forys ◽  
Barbara Trzebicka ◽  
Adam Alemayehu ◽  
Vaclav Tyrpekl ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Ethylene Glycol ◽  
Hybrid Nanostructures ◽  
Trimethylammonium Chloride ◽  
Dna Nanostructures ◽  
Glycol Methacrylate ◽  
Transmission Electron ◽  
Oppositely Charged ◽  
Vinyl Benzyl

We report on the preparation of novel and multifunctional hybrid spherical-shaped nanostructures involving a double-hydrophilic block copolymer, namely the neutral cationic poly[oligo(ethylene glycol) methacrylate]-b-poly[(vinyl benzyl trimethylammonium chloride)] (POEGMA-b-PVBTMAC) diblock copolymer, initially complexed with hydrophilic anionic magnetic nanoparticles (MNPs), and subsequently, with short deoxyribonucleic acid (113 bases DNA). The POEGMA-b-PVBTMAC copolymer, the copolymer/MNPs and the copolymer/MNPs/DNA tricomponent hybrid electrostatic complexes were studied by dynamic/electrophoretic light scattering (DLS/ELS) and cryogenic transmission electron microscopy (cryo-TEM) techniques for the determination of their structure and solution properties. The MNPs were complexed efficiently with the oppositely charged diblock chains, leading to well-defined hybrid organic–inorganic spherical-shaped nanostructures. A significant aggregation tendency of the MNPs is noticed in cryo-TEM measurements after the electrostatic complexation of DNA, implying an accumulation of the DNA macromolecules on the surface of the hybrid tricomponent complexes. Magnetophoretic experiments verified that the MNPs maintain their magnetic properties after the complexation initially with the copolymer, and subsequently, within the block polyelectrolyte/MNPs/DNA nanostructures.

scholarly journals Small nanosized poly(vinyl benzyl trimethylammonium chloride) based polyplexes for siRNA delivery

2017 ◽  
Vol 525 (2) ◽  
pp. 388-396 ◽  
Author(s):  
Bo Lou ◽  
Nataliia Beztsinna ◽  
Grigoris Mountrichas ◽  
Joep B. van den Dikkenberg ◽  
Stergios Pispas ◽  
...  
Keyword(s):  
Sirna Delivery ◽  
Trimethylammonium Chloride ◽  
Vinyl Benzyl

Studies in the Alismataceae. V. Experimental modification of phyllotaxis in pseudostolons of Echinodorus tenellus by means of growth inhibitors

1974 ◽  
Vol 52 (5) ◽  
pp. 1131-1142 ◽  
Author(s):  
W. A. Charlton
Keyword(s):  
Stable State ◽  
Main Axis ◽  
Trimethylammonium Chloride ◽  
Succinamic Acid ◽  
Lateral Buds ◽  
Experimental Findings ◽  
High Degree ◽  
Vegetative Bud ◽  
Experimental Modification

Treatment with triiodobenzoic acid (TIBA), N-dimethylamino succinamic acid (B-995), and (2-chloroethyl)trimethylammonium chloride (CCC) can modify the pattern of development of the pseudostolons of Echinodorus tenellus. In particular, CCC and B-995 can with a high degree of success suppress the normal reversal of genetic spiral of the pseudostolon. Other aspects of phyllotaxis, including phyllotaxis of lateral buds, are not affected by CCC and B-995.The pseudostolons, as previously described, are modified inflorescences bearing bracts in false whorls of three, and a vegetative bud in the axil of the first bract of each set after the first. After passing through a brief stabilizing phase the pseudostolon enters a stable state in which the direction of genetic spiral of the main axis reverses with the initiation of the second bract of each set, and in which the genetic spiral of the lateral vegetative bud is quite consistently homodromous with that of the main axis at the time of initiation of the bud.The experimental findings are discussed in relation to the earlier descriptive and analytical studies of E. tenellus. Some previously erected hypotheses relating to control of phyllotaxis in this plant are invalidated. It is suggested that in E. tenellus determination of maintenance or reversal of genetic spiral occurs within a short span of development between the initiation of the first and second bract of each set, and that direction of genetic spiral in the pseudostolon and its vegetative laterals may be controlled by mechanisms independent of those controlling other aspects of phyllotaxis such as divergence angle.

Specific micro-radioimmunoassay for prednisolone in serum.

1983 ◽  
Vol 29 (7) ◽  
pp. 1358-1362 ◽  
Author(s):  
A Olivesi ◽  
D S Smith ◽  
G W White ◽  
M Pourfarzaneh
Keyword(s):  
Chromatographic Method ◽  
Trimethylammonium Chloride ◽  
Chemical Derivatization ◽  
Liquid Chromatographic Method ◽  
Selective Chemical ◽  
Girard’S Reagent ◽  
Liquid Chromatographic

Abstract Use of an 125I radioimmunoassay involving antiserum coupled to magnetizable cellulose, after prednisolone-21-phosphate interference is removed by dichloromethane extraction at pH 7.4 and endogenous glucocorticoid interference is removed by selective chemical derivatization with Girard's Reagent T [(carboxymethyl)trimethylammonium chloride hydrazide], allows determination of prednisolone in 10 microL of serum. Results correlate well with those of an established liquid-chromatographic method for separating prednisolone from its metabolites.

scholarly journals Size of Heparin-Imprinted Nanoparticles Reflects the Matched Interactions with the Target Molecule

Sensors ◽  
2019 ◽  
Vol 19 (10) ◽  
pp. 2415 ◽  
Author(s):  
Yasuo Yoshimi ◽  
Daichi Oino ◽  
Hirofumi Ohira ◽  
Hitoshi Muguruma ◽  
Ewa Moczko ◽  
...  
Keyword(s):  
High Selectivity ◽  
Specific Interaction ◽  
Trimethylammonium Chloride ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Target Molecule ◽  
Gate Effect ◽  
New Applications ◽  
Very High

It has been shown that the faradic current at an electrode grafted with molecularly imprinted polymer (MIP) is sensitive to the specific target molecule used as the template. This phenomenon is applicable to sensors with very high selectivity, but the sensing mechanism is still a black box. We investigated the size sensitivity of nanoparticles of molecularly imprinted polymers (MIP-NPs) to a specific interaction for determination of the mechanism of the gate effect and its feasibility for new applications. Nanoparticles of poly(methacryloxy ethyl trimethylammonium chloride-co-acrylamide-co-methylenebisacrylamide) imprinted with heparin immobilized on glass beads were synthesized. The diameter of the MIP-NPs of heparin was increased by the presence of the heparin template but was insensitive to chondroitin sulfate C (CSC), the analogue of heparin. The high selectivity of the MIP-NPs was consistent with the selectivity of electrodes grafted with a heparin-imprinted polymer in our previous studies. The quartz crystal microbalance probes immobilizing heparin or CSC were sensitive to MIP-NPs, which indicates that the binding ability of MIP-NP does not discriminate between the template and other glycosaminoglycans. These results indicate that the size of the MIP-NP is sensitive to the matched binding with the template through the imprinted cavity.

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