A Convenient Method for the Synthesis of the Amphiphilic Triblock Copolymer Poly(L -lactic acid)-block- Poly(L -lysine)-block- Poly(ethylene glycol) Monomethyl Ether

2011 ◽  
Vol 212 (6) ◽  
pp. 563-573 ◽  
Author(s):  
Li Xiang ◽  
Lin-Jing Shen ◽  
Feng Long ◽  
Ke Yang ◽  
Jun-Bing Fan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Ethylene Glycol ◽  
Triblock Copolymer ◽  
Monomethyl Ether ◽  
Poly Ethylene Glycol ◽  
Ethylene Glycol Monomethyl Ether ◽  
Glycol Monomethyl Ether ◽  
Amphiphilic Triblock Copolymer ◽  
Poly Ethylene ◽  
Copolymer Poly

Preparation and Properties of a Hydrolytically Stable Cyclooctyne-Containing Polymer

Synlett ◽  
2018 ◽  
Vol 29 (19) ◽  
pp. 2535-2541
Author(s):  
Alex Adronov ◽  
Kelvin Li ◽  
Stuart McNelles
Keyword(s):  
Molecular Weight ◽  
Ethylene Glycol ◽  
Monomethyl Ether ◽  
Phenylene Vinylene ◽  
Cross Linking ◽  
Poly Ethylene Glycol ◽  
Ethylene Glycol Monomethyl Ether ◽  
Glycol Monomethyl Ether ◽  
Poly Ethylene

A poly[(phenylene vinylene)-co-dibenzocyclooctyne] polymer prepared by Wittig polymerization chemistry between dibenzocyclooctyne bisaldehyde [DIBO-(CHO)2] and bis(triethyleneglycol)phenylbis(tributylphosphonium) dibromide is reported. The resulting polymer exhibits moderate molecular weight (Mn: 10.5 kDa, Mw: 21.3 kDa, Ð: 2.02) and is fluorescent. It could be readily functionalized by strain-promoted alkyne-azide cycloadditon with different azides, and fluorescence of the polymer was preserved after functionalization. Grafting azide-terminated 5 kDa poly(ethylene glycol) monomethyl ether chains drastically affected the solubility of the polymer. Cross-linking the polymer with poly(ethylene glycol) that was terminated at both ends with azide groups gave access to a fluorescent organogel that could be dried and reswollen with water to form a hydrogel.

scholarly journals rAAV-Mediated Overexpression of SOX9 and TGF-β via Carbon Dot-Guided Vector Delivery Enhances the Biological Activities in Human Bone Marrow-Derived Mesenchymal Stromal Cells

Nanomaterials ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 855 ◽  
Author(s):  
Weikun Meng ◽  
Ana Rey-Rico ◽  
Mickaël Claudel ◽  
Gertrud Schmitt ◽  
Susanne Speicher-Mentges ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Citric Acid ◽  
Ethylene Glycol ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Monomethyl Ether ◽  
Poly Ethylene Glycol ◽  
Ethylene Glycol Monomethyl Ether ◽  
Glycol Monomethyl Ether ◽  
Poly Ethylene

Scaffold-assisted gene therapy is a highly promising tool to treat articular cartilage lesions upon direct delivery of chondrogenic candidate sequences. The goal of this study was to examine the feasibility and benefits of providing highly chondroreparative agents, the cartilage-specific sex-determining region Y-type high-mobility group 9 (SOX9) transcription factor or the transforming growth factor beta (TGF-β), to human bone marrow-derived mesenchymal stromal cells (hMSCs) via clinically adapted, independent recombinant adeno-associated virus (rAAV) vectors formulated with carbon dots (CDs), a novel class of carbon-dominated nanomaterials. Effective complexation and release of a reporter rAAV-lacZ vector was achieved using four different CDs elaborated from 1-citric acid and pentaethylenehexamine (CD-1); 2-citric acid, poly(ethylene glycol) monomethyl ether (MW 550 Da), and N,N-dimethylethylenediamine (CD-2); 3-citric acid, branched poly(ethylenimine) (MW 600 Da), and poly(ethylene glycol) monomethyl ether (MW 2 kDa) (CD-3); and 4-citric acid and branched poly(ethylenimine) (MW 600 Da) (CD-4), allowing for the genetic modification of hMSCs. Among the nanoparticles, CD-2 showed an optimal ability for rAAV delivery (up to 2.2-fold increase in lacZ expression relative to free vector treatment with 100% cell viability for at least 10 days, the longest time point examined). Administration of therapeutic (SOX9, TGF-β) rAAV vectors in hMSCs via CD-2 led to the effective overexpression of each independent transgene, promoting enhanced cell proliferation (TGF-β) and cartilage matrix deposition (glycosaminoglycans, type-II collagen) for at least 21 days relative to control treatments (CD-2 lacking rAAV or associated to rAAV-lacZ), while advantageously restricting undesirable type-I and -X collagen deposition. These results reveal the potential of CD-guided rAAV gene administration in hMSCs as safe, non-invasive systems for translational strategies to enhance cartilage repair.

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