Translational Research in Hepatopulmonary Syndrome: Lessons Learned Despite Negative Results

2019 ◽  
Vol 25 (8) ◽  
pp. 1136-1137
Author(s):  
Karen L. Swanson
Keyword(s):  
Translational Research ◽  
Hepatopulmonary Syndrome ◽  
Lessons Learned ◽  
Negative Results

Bridging between academia and the workplace: lessons learned about translational research on work-family balance

2019 ◽  
Vol 22 (4) ◽  
pp. 465-477 ◽  
Author(s):  
Nathalie Houlfort ◽  
Mélanie Lefrançois ◽  
Stéphanie Bernstein ◽  
Karen Messing ◽  
Anne-Renée Gravel ◽  
...  
Keyword(s):  
Translational Research ◽  
Lessons Learned ◽  
Family Balance ◽  
Work Family Balance ◽  
Work Family

scholarly journals Towards Multitarget Testing in Molecular Microbiology

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Deborah Steensels ◽  
Anne Vankeerberghen ◽  
Hans De Beenhouwer
Keyword(s):  
False Negative ◽  
Lessons Learned ◽  
Patient Treatment ◽  
Culture Methods ◽  
Diagnostic Assays ◽  
Genome Variability ◽  
Negative Results ◽  
Conventional Culture ◽  
False Negative Results ◽  
Single Target

Advantages of PCR assays over more conventional culture-based diagnostics include significantly higher sensitivities and shorter turnaround times. They are particularly useful when patient treatment has already been initiated or for specimens that may contain microorganisms that are slow-growing, difficult to culture, or for which culture methods do not exist. However, due to genome variability, single target testing might lead to false-negative results. This paper focuses on examples from our own experiences and the literature to provide insight into the limitations of single target testing in molecular biology. Lessons learned from these experiences include the careful design of diagnostic assays, preferably multitargeted, the importance of investigating the incidence and epidemiology of infection in detail, the frequent participation in appropriate quality assurance schemes, and the importance of continuous attentiveness by investigators when confronted with inconsistent results. In conclusion, multitargeted testing in microbiological molecular assays should be a rule.

scholarly journals Lessons learned from developing and sustaining a community-research collaborative through translational research

2018 ◽  
Vol 2 (2) ◽  
pp. 79-85 ◽  
Author(s):  
Amy LeClair ◽  
Jean J. Lim ◽  
Carolyn Rubin
Keyword(s):  
Translational Research ◽  
Participatory Research ◽  
Community Based Participatory Research ◽  
Lessons Learned ◽  
Community Research ◽  
Structured Interviews ◽  
Community Based ◽  
Spheres Of Influence ◽  
Asian Populations ◽  
Current State

IntroductionThe goal of this project was to document the current state of a community-academic partnership, identifying early successes and lessons learned.MethodsWe employed qualitative methods, semi-structured interviews and document analysis, from 2 data sources to (1) show how the principles of community-based participatory research are enacted through the activities of Addressing Disparities in Asian Populations through Translational Research (ADAPT) and (2) elucidate the barriers and facilitators to adhering to those principles from the perspectives of the members themselves.ResultsIn addition to established community-based participatory research values, understanding individuals’ motivations for participation, the challenges aligning the priorities of community organizations and academic partners, and definitions of success are themes that emerged as key to the process of maintaining this partnership.ConclusionAs the emphasis on community-academic partnerships grows, there is potential for clinical and translational science awards to use community engagement to facilitate translational research beyond the traditional medical spheres of influence and to forge relationships with affected communities.

scholarly journals Response: Some lessons learned from negative results of a randomized controlled trial for bladder reinnervation with the Xiao procedure

2016 ◽  
Vol 18 (2) ◽  
pp. 148-149
Author(s):  
Gerald F. Tuite ◽  
Ethan G. Polsky ◽  
Yves Homsy ◽  
Margaret A. Reilly ◽  
Carolyn M. Carey ◽  
...  
Keyword(s):  
Randomized Controlled Trial ◽  
Controlled Trial ◽  
Lessons Learned ◽  
Randomized Controlled ◽  
Negative Results

Improving staff morale through authentic appreciation

2014 ◽  
Vol 28 (5) ◽  
pp. 17-20 ◽  
Author(s):  
Paul White
Keyword(s):  
Employee Engagement ◽  
Lessons Learned ◽  
Staff Morale ◽  
Content Type ◽  
Employee Recognition ◽  
Negative Results ◽  
Employee Values ◽  
Alternative Approach ◽  
Core Components ◽  
Traditional Approaches

Purpose – This paper aims to address the increasingly low levels of staff morale found in workplaces and the challenges managers have. Employees tend to view employee recognition programs cynically and the reasons for these reactions are explained, along with the negative results which follow. The concept of authentic appreciation is discussed, and the core components necessary for employees to feel truly valued and practical steps that can be taken are outlined. Design/methodology/approach – The paper reports lessons learned through the author’s experiences of applying the concepts to workplaces over the past several years. Findings – Job satisfaction and employee engagement are declining in spite of the proliferation of employee recognition programs. Employees perceive much employee recognition activity as being disingenuous, leading to apathy and sarcasm. There are structural issues that need to be corrected for employee recognition to be perceived as authentic – making recognition less generic, more individualized and communicated regularly in the manner that is valued by the recipients. Practical implications – Traditional approaches to employee recognition (awards and rewards) need to be re-evaluated. Continuing these activities may actually increase the negativity within a work environment. Learning what each individual employee values and then communicating appreciation to them in ways that are perceived as authentic is critical to having a positive result. Originality/value – The paper challenges the current (and growing) trend of impersonal employee recognition programs and examines the factors that contribute to recognition being perceived as inauthentic. The author then provides an alternative approach and methodology that facilitates the ability to communicate authentic appreciation.

scholarly journals Skin Swabbing for Staphylococcus aureus–Targeting Phages

2021 ◽  
Author(s):  
Christopher Duplessis ◽  
Thomas C Luke ◽  
Chase Watters ◽  
Yolanda Alcorta ◽  
Biswajit Biswas
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Pathogen ◽  
Soft Agar ◽  
Positive Control ◽  
Lessons Learned ◽  
Phage Library ◽  
Negative Results ◽  
Institutional Review ◽  
Anatomic Locations ◽  
Wound Effluent

ABSTRACT Introduction Staphylococcus aureus (SA) is a major human bacterial pathogen increasingly refractory to antibiotics. Given the dearth of novel antibiotics in the developmental pipeline, we require concerted efforts at optimizing novel antimicrobial approaches. One promising option is the utilization of bacteriophage (phage) therapy, which has been resurrected as a viable clinical therapeutic. Specifically, an expanded library of phages targeting SA is desired. We surmised that SA-targeting phages would be readily accessible as a major component of the cutaneous microbiome. Specifically, we sought to discern if easily accessible (convenient) and discrete anatomic locations, including the nares, axilla, fingernails, toenails, and web spaces, could provide intact phages via a noninvasive, expedient procedure involving swabbing. Methods One hundred subjects participated in systematic skin swab specimen collections. Pooled samples were subject to phage harvesting utilizing the soft agar overlay technique. The approval was secured from the Naval Medical Research Center Institutional Review Board (NMRC 2018.0004 FWA00000152). We utilized the same procedures from known samples containing SA-targeting phages. As another positive control, we employed the same swab and acquired samples from an active wound infection. Results As anticipated, there were no adverse events, and the procedure was successfully implemented within the projected 10-minute duration. No phages were identified exploiting this methodology. Positive controls from various environmental samples identified SA-targeting phages as did the wound effluent sample. Conclusions Skin swabbing at multiple anatomic sites from 100 adults yielded insufficient biomass for phage recovery. The negative results provide helpful information for future phage isolation attempts. The lessons learned on why this study failed to isolate phages can be easily utilized by others. With a desire to increase our SA-targeting phage library in pursuit of future clinical trials, and acknowledging the paucity of these phages accessible via traditional recovery from environmental sources, we will next acquire large volumes of wound effluent from confirmed infected wounds with SA to optimize the biomass for phage recovery.

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