scholarly journals Rapid, large-scale formation of porcine hepatocyte spheroids in a novel spheroid reservoir bioartificial liver

2005 ◽  
Vol 11 (8) ◽  
pp. 901-910 ◽  
Author(s):  
Scott L. Nyberg ◽  
Joseph Hardin ◽  
Bruce Amiot ◽  
Upendra A. Argikar ◽  
Rory P. Remmel ◽  
...  
Keyword(s):  
Large Scale ◽  
Bioartificial Liver ◽  
Scale Formation ◽  
Porcine Hepatocyte ◽  
Hepatocyte Spheroids

Comparisons of Porcine Hepatocyte Spheroids and Single Hepatocytes in the Non-Woven Fabric Bioartificial Liver Module

1996 ◽  
Vol 19 (10) ◽  
pp. 605-609 ◽  
Author(s):  
K. Naruse ◽  
Y. Sakai ◽  
I. Nagashima ◽  
G.X. Jiang ◽  
M. Suzuki ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Bioartificial Liver ◽  
Woven Fabric ◽  
Culture Vessel ◽  
Lower Efficiency ◽  
Pig Liver ◽  
Cell Basis ◽  
Porcine Hepatocyte ◽  
Hepatocyte Spheroids

We previously developed a new bioreactor of the bioartificial liver composed of non-woven fabric. We have also experimented with hepatocyte spheroids, with the aim of improving the efficiency of this NWF bioreactor. In this study, we compared the efficiencies of NWF bioreactors employing hepatocyte spheroids versus single hepatocytes. Hepatocytes were isolated from a whole pig liver by Seglen's method. 1.0 × 1010 single hepatocytes were immobilized in the NWF bioreactor. Another 1.0 × 1010 hepatocytes were allowed to form spheroids by 24 hr suspension culture in a 4-L culture vessel, before being immobilized in the bioreactor. Hepatocyte spheroids were found to be functionally superior, on a per-cell basis, to single hepatocytes in the NWF bioreactor. However, the NWF bioreactor employing hepatocyte spheroids exhibited lower efficiency than that employing single hepatocytes, because the total number of the hepatocytes had decreased during the 24 hr suspension culture.

scholarly journals Cold storage of porcine hepatocyte spheroids for spheroid bioartificial liver

2019 ◽  
Vol 26 (4) ◽  
Author(s):  
Yi Li ◽  
Harvey S. Chen ◽  
Mohammed Shaheen ◽  
Dong Jin Joo ◽  
Bruce P. Amiot ◽  
...  
Keyword(s):  
Cold Storage ◽  
Bioartificial Liver ◽  
Porcine Hepatocyte ◽  
Hepatocyte Spheroids

Hollow fiber bioartificial liver utilizing collagen-entrapped porcine hepatocyte spheroids

1996 ◽  
Vol 52 (1) ◽  
pp. 34-44 ◽  
Author(s):  
Florence J. Wu ◽  
Julie R. Friend ◽  
Arye Lazar ◽  
Henry J. Mann ◽  
Rory P. Remmel ◽  
...  
Keyword(s):  
Hollow Fiber ◽  
Bioartificial Liver ◽  
Porcine Hepatocyte ◽  
Hepatocyte Spheroids

Ammonium Detoxification Performed by Porcine Hepatocyte Spheroids in a Bioartificial Liver for Pediatric Use: Preliminary Report

2003 ◽  
Vol 27 (7) ◽  
pp. 665-670 ◽  
Author(s):  
Alicia Lorenti ◽  
Mariana Barbich ◽  
Martin de Santibanes ◽  
Marcelo Ielpi ◽  
Juan Carlos Vazquez ◽  
...  
Keyword(s):  
Preliminary Report ◽  
Bioartificial Liver ◽  
Porcine Hepatocyte ◽  
Pediatric Use ◽  
Hepatocyte Spheroids

Formation of Porcine Hepatocyte Spheroids for use in a Bioartificial Liver

1995 ◽  
Vol 4 (3) ◽  
pp. 259-268 ◽  
Author(s):  
Arye Lazar ◽  
Madhusudan V. Peshwa ◽  
Florence J. Wu ◽  
Chung-Ming Chi ◽  
Frank B. Cerra ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scaling Up ◽  
Bioartificial Liver ◽  
Multicellular Spheroids ◽  
Morphological Studies ◽  
Porcine Hepatocyte ◽  
Defined Culture ◽  
Hepatocyte Spheroids ◽  
Bioartificial Liver Device

Xenogeneic hepatocytes have recently been used in a bioartificial liver device as a potential short-term extracorporeal support of acute liver failure. Scaling up the system requires large quantities of viable and highly active cells. Hepatocytes grown as spheroids manifest higher metabolic activities for longer time periods as compared to those in monolayer cultures. Use of hepatocyte spheroids for application in a bioartificial liver can possibly alleviate the need of scaling up. Porcine hepatocytes when cultured under stirred conditions, form multicellular spheroids in a defined culture medium. Spheroids were formed 24 h after cell inoculation with an efficiency of 80-90% and a mean diameter of about 135 μm. Scanning electron microscopy revealed numerous microvilli projecting from the entire surface of the spheroids. Transmission electron microscopy revealed differentiated hepatocytes which displayed well-developed cytoplasmic structures separated by bile canaliculus-like structures. The morphological studies show a resemblance between cells in the spheroids and in the liver in vivo. Ureagenesis by spheroids was twice as active and was sustained for a longer culture period than that by hepatocytes cultured as monolayers. Preparation of porcine hepatocyte spheroids in an agitated vessel is simple efficient and reproducible. It will allow for preparation of large quantities of spheroids to be employed in a bioartificial liver device as well as in liver metabolism studies.

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