Hair regrowth through wound healing process after ablative fractional laser treatment in a murine model

2015 ◽  
Vol 47 (5) ◽  
pp. 433-440 ◽  
Author(s):  
Jung Min Bae ◽  
Han Mi Jung ◽  
Boncheol Goo ◽  
Young Min Park
Keyword(s):  
Wound Healing ◽  
Laser Treatment ◽  
Murine Model ◽  
Healing Process ◽  
Wound Healing Process ◽  
Fractional Laser ◽  
Hair Regrowth ◽  
Ablative Fractional Laser

scholarly journals Wound Healing Activity of the Essential Oil of Bursera morelensis, in Mice

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1795
Author(s):  
Judith Salas-Oropeza ◽  
Manuel Jimenez-Estrada ◽  
Armando Perez-Torres ◽  
Andres Eliu Castell-Rodriguez ◽  
Rodolfo Becerril-Millan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Essential Oil ◽  
Cell Viability ◽  
Murine Model ◽  
Biological Activities ◽  
Healing Process ◽  
In Vitro Tests ◽  
Wound Healing Process ◽  
Fibroblast Migration

Bursera morelensis is used in Mexican folk medicine to treat wounds on the skin. It is an endemic tree known as “aceitillo”, and the antibacterial and antifungal activity of its essential oil has been verified; it also acts as an anti-inflammatory. All of these reported biological activities make the essential oil of B. morelensis a candidate to accelerate the wound-healing process. The objective was to determine the wound-healing properties of B. morelensis’ essential oil on a murine model. The essential oil was obtained by hydro-distillation, and the chemical analysis was performed by gas chromatography-mass spectrometry (GC-MS). In the murine model, wound-healing efficacy (WHE) and wound contraction (WC) were evaluated. Cytotoxic activity was evaluated in vitro using peritoneal macrophages from BALB/c mice. The results showed that 18 terpenoid-type compounds were identified in the essential oil. The essential oil had remarkable WHE regardless of the dose and accelerated WC and was not cytotoxic. In vitro tests with fibroblasts showed that cell viability was dose-dependent; by adding 1 mg/mL of essential oil (EO) to the culture medium, cell viability decreased below 80%, while, at doses of 0.1 and 0.01 mg/mL, it remained around 90%; thus, EO did not intervene in fibroblast proliferation, but it did influence fibroblast migration when wound-like was done in monolayer cultures. The results of this study demonstrated that the essential oil was a pro-wound-healing agent because it had good healing effectiveness with scars with good tensile strength and accelerated repair. The probable mechanism of action of the EO of B. morelensis, during the healing process, is the promotion of the migration of fibroblasts to the site of the wound, making them active in the production of collagen and promoting the remodeling of this collagen.

scholarly journals Transcriptomic analysis of human skin wound healing and rejuvenation following ablative fractional laser treatment

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0260095
Author(s):  
Joseph D. Sherrill ◽  
Deborah Finlay ◽  
Robert L. Binder ◽  
Michael K. Robinson ◽  
Xingtao Wei ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wound Healing ◽  
Laser Treatment ◽  
Human Skin ◽  
Fractional Laser ◽  
Skin Rejuvenation ◽  
Skin Wound Healing ◽  
Age Related ◽  
Ablative Fractional Laser ◽  
Laser Treatments

Ablative fractional laser treatment is considered the gold standard for skin rejuvenation. In order to understand how fractional laser works to rejuvenate skin, we performed microarray profiling on skin biopsies to identify temporal and dose-response changes in gene expression following fractional laser treatment. The backs of 14 women were treated with ablative fractional laser (Fraxel®) and 4 mm punch biopsies were collected from an untreated site and at the treated sites 1, 3, 7, 14, 21 and 28 days after the single treatment. In addition, in order to understand the effect that multiple fractional laser treatments have on skin rejuvenation, several sites were treated sequentially with either 1, 2, 3, or 4 treatments (with 28 days between treatments) followed by the collection of 4 mm punch biopsies. RNA was extracted from the biopsies, analyzed using Affymetrix U219 chips and gene expression was compared between untreated and treated sites. We observed dramatic changes in gene expression as early as 1 day after fractional laser treatment with changes remaining elevated even after 1 month. Analysis of individual genes demonstrated significant and time related changes in inflammatory, epidermal, and dermal genes, with dermal genes linked to extracellular matrix formation changing at later time points following fractional laser treatment. When comparing the age-related changes in skin gene expression to those induced by fractional laser, it was observed that fractional laser treatment reverses many of the changes in the aging gene expression. Finally, multiple fractional laser treatments, which cover different regions of a treatment area, resulted in a sustained or increased dermal remodeling response, with many genes either differentially regulated or continuously upregulated, supporting previous observations that maximal skin rejuvenation requires multiple fractional laser treatments. In conclusion, fractional laser treatment of human skin activates a number of biological processes involved in wound healing and tissue regeneration.

scholarly journals Transcriptomic Analysis of Human Skin Wound Healing and Rejuvenation Following Ablative Fractional Laser Treatment

2021 ◽  
Author(s):  
Joseph D Sherrill ◽  
Deborah Finlay ◽  
Robert L Binder ◽  
Michael K Robinson ◽  
Xingtao Wei ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wound Healing ◽  
Laser Treatment ◽  
Skin Wound ◽  
Fractional Laser ◽  
Skin Rejuvenation ◽  
Skin Wound Healing ◽  
Age Related ◽  
Ablative Fractional Laser ◽  
Laser Treatments

Ablative fractional laser treatment is considered the gold standard for skin rejuvenation. In order to understand how fractional laser works to rejuvenate skin, we performed microarray profiling on skin biopsies to identify temporal and dose-response changes in gene expression following fractional laser treatment. The backs of 14 women were treated with ablative fractional laser (Fraxel) and 4 mm punch biopsies were collected from an untreated site and at the treated sites 1, 3, 7, 14, 21 and 28 days after the single treatment. In addition, in order to understand the effect that multiple fractional laser treatments have on skin rejuvenation, several sites were treated sequentially with either 1, 2, 3, or 4 treatments (with 28 days between treatments) followed by the collection of 4 mm punch biopsies. RNA was extracted from the biopsies, analyzed using Affymetrix U219 chips and gene expression was compared between untreated and treated sites. We observed dramatic changes in gene expression as early as 1 day after fractional laser treatment with changes remaining elevated even after 1 month. Analysis of individual genes demonstrated significant and time related changes in inflammatory, epidermal, and dermal genes, with dermal genes linked to extracellular matrix formation changing at later time points following fractional laser treatment. When comparing the age-related changes in skin gene expression to those induced by fractional laser, it was observed that fractional laser treatment reverses many of the changes in the aging gene expression. Finally, multiple fractional laser treatments resulted in continued changes in gene expression, with many genes either differentially regulated or continuously upregulated with increasing number of treatments, indicating that maximal skin rejuvenation requires multiple fractional laser treatments. In conclusion, fractional laser treatment of skin activates several biological processes involved in wound healing and tissue regeneration, all of which significantly contribute to the rejuvenating effect of fractional laser treatment on aged skin.

scholarly journals Monitoring of wound healing process of human skin after fractional laser treatments with optical coherence tomography

2013 ◽  
Vol 4 (11) ◽  
pp. 2362 ◽  
Author(s):  
Meng-Tsan Tsai ◽  
Chih-Hsun Yang ◽  
Su-Chin Shen ◽  
Ya-Ju Lee ◽  
Feng-Yu Chang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Optical Coherence Tomography ◽  
Human Skin ◽  
Healing Process ◽  
Wound Healing Process ◽  
Optical Coherence ◽  
Fractional Laser ◽  
Laser Treatments

scholarly journals Effect of Adjuvant Imiquimod 5% Cream on Sustained Clearance of Anogenital Warts Following Laser Treatment

2001 ◽  
Vol 10 (2) ◽  
pp. 79-88 ◽  
Author(s):  
U. B. Hoyme ◽  
M. Hagedorn ◽  
A.-E. Schindler ◽  
P. Schneede ◽  
W. Hopfenmüller ◽  
...  
Keyword(s):  
Wound Healing ◽  
Laser Treatment ◽  
Laser Therapy ◽  
Healing Process ◽  
Primary Objective ◽  
Treatment Modalities ◽  
Wound Healing Process ◽  
Anogenital Warts ◽  
Open Label ◽  
Number Of Patients

Objectives:Imiquimod is an immune response modifier that has demonstrated a good efficacy and relatively low recurrencerates in comparison to other genitalwart treatment modalities. The primary objective of this open-label study was to evaluate the effect on sustained clearance of treated lesions and the safety of patient-applied topical imiquimod after laser therapy of external anogenital warts.Methods:After laser treatment of visible external anogenital warts the ablated region(s) were treated with imiquimod 5% cream three times/week over 12 weeks beginningwhen the wound healing process was completed, followed by a six-month treatment-free observation period for the assessment of sustained clearance of treated lesions.Results:A total of 211 male and female patients was enrolled in the study. After 12 weeks of treatment, 65.4% of all patients showed sustained clearance. During the treatment period, 15 patients (7.1% of 211 patients) presented with recurrent warts in the treated areas, and 58 (27.5%) patients were excluded for other reasons. During the six-month follow-up period, ten additional patients (7.3% of 138 patients) developed wart recurrences. The application of imiquimod 5% cream was well tolerated. The number of patients with adverse events related to studymedication declined fromthe first month of treatment until the end of the third month.Most frequently, mild to moderate itching, burning, pain and erythema were reported.Conclusions:After laser therapy and sufficient wound healing, administration of imiquimod 5% cream three times/week appears to be safe and to reduce the incidence of wart recurrences.

scholarly journals The acute phase of inflammatory response involved in the wound-healing process after excimer laser treatment

2016 ◽  
pp. 993 ◽  
Author(s):  
Bojan Pajic ◽  
Mirko Resan ◽  
Miroslav Vukosavljevic ◽  
Brigitte Pajic-Eggspuehler ◽  
Danilo Vojvodic
Keyword(s):  
Wound Healing ◽  
Inflammatory Response ◽  
Laser Treatment ◽  
Excimer Laser ◽  
Acute Phase ◽  
Healing Process ◽  
Wound Healing Process

Promotion of wound healing through low-fluence ablative fractional laser treatment in diabetic mice

2018 ◽  
Vol 34 (2) ◽  
pp. 421-425 ◽  
Author(s):  
Han Na Lee ◽  
Jung Min Bae ◽  
Bon Cheol Leo Goo ◽  
Young Min Park
Keyword(s):  
Wound Healing ◽  
Laser Treatment ◽  
Diabetic Mice ◽  
Fractional Laser ◽  
Ablative Fractional Laser

scholarly journals Wound Healing Process in the Portio Vaginalis Uteri after CO2-Laser Treatment.

1993 ◽  
Vol 170 (3) ◽  
pp. 135-146 ◽  
Author(s):  
KAZUHIRO HOSHI ◽  
SHIZUKA HONDA ◽  
CHIKARA ENDO ◽  
AKIRA SATO ◽  
SHOJI SEKIMOTO
Keyword(s):  
Wound Healing ◽  
Laser Treatment ◽  
Co2 Laser ◽  
Healing Process ◽  
Wound Healing Process ◽  
Co2 Laser Treatment

HUBUNGAN MOBILISASI DINI TERHADAP PENYEMBUHAN LUKA POST OPERASI SECTIO CESARIA

2019 ◽  
Vol 2 (1) ◽  
pp. 14-17
Author(s):  
Nurul Aini Siagian ◽  
Syafira Nusaibah ◽  
Andayani Boang Manalu
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Early Mobilization ◽  
Sectio Caesarea ◽  
Wound Healing Process ◽  
P Value ◽  
Cross Sectional ◽  
Care Services ◽  
Healing Wound ◽  
Rapid Healing

Early mobilization includes factors that can affect the process of wound healing after surgery. Immediate mobilization in stages is very useful for the process of healing wounds and preventing infection and venous thrombosis. The purpose of this study was to determine whether there is a relationship between early mobilization and the process of healing wound post operative sectio caesarea at Sinar Husni General Hospital Medan Helvetia. The research design used was analytic survey with cross sectional approach. The sample in this study used the Consecutive Sampling method of data collection using a checklist sheet conducted on a sample of 19 respondents. The results of the study in this study are the majority of respondents who did early mobilization and who experienced rapid wound healing process as many as 4 people (21%) while the minority of respondents who did early mobilization and who experienced slow wound healing process were 1 person (5.3%). The majority of respondents who did not mobilize early and who experienced a slow wound healing process were 11 people (57.9%) and a minority who did not mobilize early and who experienced rapid healing as many as 4 people (21.1%). Statistical test results obtained p value = 0.046 <0.005. The conclusions of the results of this study indicate there is a relationship between early mobilization and the process of healing post operative sectio of caesarea. Suggestions The results of this study can be applied as a reference to improve nursing care services, especially in providing counseling and assistance to patients.

Hubungan Status Gizi Dengan Proses Penyembuhan Luka Di Rsud H. Andi Sulthan Daeng Radja Kabupaten Bulukumba

2017 ◽  
Vol 2 (1) ◽  
pp. 20-28
Author(s):  
Fitriani ◽  
Tenriwati
Keyword(s):  
Wound Healing ◽  
Nutritional Status ◽  
Healing Process ◽  
Data Retrieval ◽  
Observational Research ◽  
Wound Healing Process ◽  
P Value ◽  
Chi Square ◽  
Cross Sectional ◽  
Chi Square Test

At present, the incidence of injuries in Indonesia is quite high, as seen from data on traffic accidents in the general public. There are several factors that affect wound healing, one of which is nutritional status. Based on the preliminary data retrieval conducted by researchers in the seruni surgery room in the last 1 month namely in March, it was found that the number of wounded patients was 109 patients, where the number of men was 78 (71.56%) while the number of women was 31 ( 28.44%) The purpose of this study was to determine the relationship between nutritional status and the wound healing process in RSUD. H. Andi. Sulthan Daeng Radja Kab. Bulukumba. This research uses the type of design of this research is quantitative research. This type of research uses analytic observational research with cross sectional approach. The sample of this study were 41 respondents taken by purposive sampling method. Data analysis in this study used the chi-square test (chi square test). The results of the analysis used the chi-square statistical test with a confidence level (α = 0.05). Based on the results of this test, the p value is 0.001, thus p <α (0.001 <0.05), then Ho is rejected and Ha is accepted. The conclusion of this study is that there is a relationship between nutritional status and the process of wound healing in RSUD. H. Andi. Sulthan Daeng Radja Kab. Bulukumba. Researchers suggest that this study be used as a material consideration in the fulfillment of nutrition in wound care patients in RSUD.H.A. Sulthan Daeng Radja Kab. Bulukumba and this research can be continued by conducting research related to the wound healing process and linking it with other variables.

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