scholarly journals Pulsed dye laser-induced inflammatory response and extracellular matrix turnover in rat vocal folds and vocal fold fibroblasts

2009 ◽  
Vol 41 (8) ◽  
pp. 585-594 ◽  
Author(s):  
Ya Lin ◽  
Masaru Yamashita ◽  
Jingxian Zhang ◽  
Changying Ling ◽  
Nathan V. Welham
Keyword(s):  
Extracellular Matrix ◽  
Inflammatory Response ◽  
Vocal Fold ◽  
Vocal Folds ◽  
Dye Laser ◽  
Pulsed Dye Laser

scholarly journals The Efficacy of Treatment for Vocal Fold Bowing with Pulsed Dye Laser

2012 ◽  
Vol 2 (1) ◽  
pp. 46-48
Author(s):  
Yong Cheol Koo ◽  
Hyo Jin Chung ◽  
Michelle J Suh ◽  
Hong-Shik Choi
Keyword(s):  
Quality Improvement ◽  
Vocal Cord ◽  
Vocal Fold ◽  
Speech Therapy ◽  
Voice Quality ◽  
Vocal Folds ◽  
Dye Laser ◽  
Liquid Silicon ◽  
Pulsed Dye Laser ◽  
Efficacy Of Treatment

ABSTRACT Bowing of the vocal folds can result from aging, atrophy or idiopathic causes, such as an injudicious vocal cord surgery. Bowing results in dysphonia due to inadequate approximation of the vocal folds. A number of treatments have been proposed for this condition. Intracordal injection of biological materials including liquid silicon and Teflon and various types of thyroplasty have been utilized. However, full voice recovery has never been fully achieved. We present a case involving a 64-year-old Asian man with dysphonia for 30 years. The patient's vocal fold bowing was examined on laryngoscopy. The disease was effectively treated with pulsed dye laser (PDL) followed by speech therapy. Observation of the patient over 1 year did not show any signs of recurrence. Our analysis revealed voice quality improvement. How to cite this article Koo YC, Chung HJ, Suh MJ, Choi HS. The Efficacy of Treatment for Vocal Fold Bowing with Pulsed Dye Laser. Int J Phonosurg Laryngol 2012;2(1):46-48.

scholarly journals The Protective Effect of Echinochrome A on Extracellular Matrix of Vocal Folds in Ovariectomized Rats

Marine Drugs ◽  
2020 ◽  
Vol 18 (2) ◽  
pp. 77
Author(s):  
Ji Min Kim ◽  
Jeong Hun Kim ◽  
Sung-Chan Shin ◽  
Gi Cheol Park ◽  
Hyung Sik Kim ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Vocal Fold ◽  
Sham Group ◽  
Vocal Folds ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Therapeutic Effects ◽  
Mrna Levels ◽  
Echinochrome A ◽  
Group I

Here, we investigated the effects of sex hormones on extracellular matrix (ECM)-related gene expression in the vocal fold lamina propria of ovariectomized (after ovary removal) rats and verified whether echinochrome A (ECH) exerts any therapeutic effects on ECM reconstitution after estrogen deficiency in ovariectomized rats. Sprague–Dawley female rats (9 weeks old) were acclimatized for a week and randomly divided into three groups (n = 15 each group) as follows: group I (sham-operated rats, SHAM), group II (ovariectomized rats, OVX), group III (ovariectomized rats treated with ECH, OVX + ECH). Rats from the OVX + ECH group were intraperitoneally injected with ECH at 10 mg/kg thrice a week after surgery for 6 weeks. And rats were sacrificed 6 weeks after ovariectomy. Estradiol levels decreased in OVX group compared with the SHAM group. ECH treatment had no effect on the levels of estradiol and expression of estrogen receptor β (ERβ). The evaluation of ECM components showed no significant changes in elastin and hyaluronic acid levels between the different groups. Collagen I and III levels were lower in OVX group than in SHAM group but increased in OVX + ECH group. The mRNA levels of matrix metalloproteinase (MMP)-1, -2, -8, and -9 were significantly higher in the OVX group than in the SHAM group, but decreased in the OVX + ECH group. Thus, changes were observed in ECM-related genes in the OVX group upon estradiol deficiency that were ameliorated by ECH administration. Thus, the vocal fold is an estradiol-sensitive target organ and ECH may have protective effects on the ECM of vocal folds in ovariectomized rats.

In Vivo Engineering of the Vocal Fold Extracellular Matrix with Injectable Hyaluronic Acid Hydrogels: Early Effects on Tissue Repair and Biomechanics in a Rabbit Model

2005 ◽  
Vol 114 (9) ◽  
pp. 662-670 ◽  
Author(s):  
Jennifer K. Hansen ◽  
Susan L. Thibeault ◽  
Jennifer F. Walsh ◽  
Xiao Zheng Shu ◽  
Glenn D. Prestwich
Keyword(s):  
Extracellular Matrix ◽  
Hyaluronic Acid ◽  
Vocal Fold ◽  
Wound Repair ◽  
Rabbit Model ◽  
Vocal Folds ◽  
Biomechanical Properties ◽  
Enzyme Linked Immunosorbent Assay ◽  
Early Effects

Objectives: A prospective, controlled animal study was performed to determine whether the use of injectable, chemically modified hyaluronic acid (HA) derivatives at the time of intentional vocal fold resection might facilitate wound repair and preserve the unique viscoelastic properties of the vocal fold extracellular matrix. Methods: We performed bilateral vocal fold biopsies on 33 rabbits. Two groups of rabbits were unilaterally treated with 2 different HA derivatives — Carbylan-SX and HA-DTPH-PEGDA — at the time of resection. Saline was injected as a control into the contralateral fold. The animals were painlessly sacrificed 3 weeks after biopsy and injection. The outcomes measured included histologic fibrosis level, tissue HA level, and tissue viscosity and elasticity. Results: The Carbylan-SX—treated vocal folds were found to have significantly less fibrosis than the saline-treated controls. The levels of HA in the treated vocal folds were not significantly different from those in the controls at 3 weeks as measured by enzyme-linked immunosorbent assay. The Carbylan-SX—treated vocal folds had significantly improved biomechanical properties of elasticity and viscosity. The HA-DTPH-PEGDA injections yielded significantly improved viscosity, but not elasticity. Conclusions: Prophylactic in vivo manipulation of the extracellular matrix with an injectable Carbylan-SX hydrogel appears to induce vocal fold tissue regeneration to yield optimal tissue composition and biomechanical properties favorable for phonation.

scholarly journals Vocal Fold Fibroblasts in Reinke’s Edema Show Alterations Involved in Extracellular Matrix Production, Cytokine Response and Cell Cycle Control

Biomedicines ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 735
Author(s):  
Magdalena Grill ◽  
Isaac Lazzeri ◽  
Andrijana Kirsch ◽  
Nina Steurer ◽  
Tanja Grossmann ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Extracellular Matrix ◽  
Differential Expression ◽  
Vocal Fold ◽  
Expression Profiles ◽  
Cell Cycle Control ◽  
Vocal Folds ◽  
Gene Set Enrichment Analysis ◽  
Current Therapy ◽  
Reinke’S Edema

The voice disorder Reinke’s edema (RE) is a smoking- and voice-abuse associated benign lesion of the vocal folds, defined by an edema of the Reinke’s space, accompanied by pathological microvasculature changes and immune cell infiltration. Vocal fold fibroblasts (VFF) are the main cell type of the lamina propria and play a key role in the disease progression. Current therapy is restricted to symptomatic treatment. Hence, there is an urgent need for a better understanding of the molecular causes of the disease. In the present study, we investigated differential expression profiles of RE and control VFF by means of RNA sequencing. In addition, fast gene set enrichment analysis (FGSEA) was performed in order to obtain involved biological processes, mRNA and protein levels of targets of interest were further evaluated. We identified 74 differentially regulated genes in total, 19 of which were upregulated and 55 downregulated. Differential expression analysis and FGSEA revealed upregulated genes and pathways involved in extracellular matrix (ECM) remodeling, inflammation and fibrosis. Downregulated genes and pathways were involved in ECM degradation, cell cycle control and proliferation. The current study addressed for the first time a direct comparison of VFF from RE to control and evaluated immediate functional consequences.

In-Office Laryngeal Surgery with the 585nm Pulsed Dye Laser (PDL)

2007 ◽  
Vol 137 (3) ◽  
pp. 477-481 ◽  
Author(s):  
Debbie Aviva Mouadeb ◽  
Peter C. Belafsky
Keyword(s):  
Vocal Fold ◽  
Common Factor ◽  
Recurrent Respiratory Papillomatosis ◽  
Dye Laser ◽  
Pulsed Dye Laser ◽  
Day Hospital ◽  
Clinical Database ◽  
Laryngeal Surgery ◽  
Promising Tool ◽  
Reinke’S Edema

OBJECTIVES: The 585nm pulsed dye laser (PDL) is a promising tool for in-office laryngeal surgery. Data with respect to the safety and efficacy of the PDL for office laryngeal use is sparse. The purpose of this study is to review our experience with unsedated office PDL surgery. METHODS: Records of individuals undergoing in-office PDL between September 1, 2004, and September 1, 2006 were abstracted from a clinical database. RESULTS: Forty-seven patients underwent 117 treatments. The most common indications were recurrent respiratory papillomatosis (RRP), Reinke's edema, and vocal fold polyps. One hundred and four of 117 procedures were felt to be a success ablating all disease. Thirteen treatments requred early termination. The most common factor responsible for termination was an inability to achieve a comfortable level of anesthesia. One patient with Reinke's edema developed postprocedure stridor that required a 3-day hospital admission for observation and corticosteroids. There was no incidence of any vocal fold scarring, web formation, or other complications. CONCLUSIONS: The 585nm PDL is a promising tool for in-office treatment of various laryngeal disorders. Complications are rare.

585-NM Pulsed Dye Laser Treatment of Glottal Papillomatosis

2002 ◽  
Vol 111 (6) ◽  
pp. 486-492 ◽  
Author(s):  
Ramon A. Franco ◽  
William A. Farinelli ◽  
Steven M. Zeitels ◽  
R. Rox Anderson
Keyword(s):  
Anterior Commissure ◽  
Vocal Folds ◽  
Spot Size ◽  
Recurrent Respiratory Papillomatosis ◽  
Dye Laser ◽  
Pulsed Dye Laser ◽  
Depth Of Penetration ◽  
Efficacious Treatment ◽  
Superficial Lamina

Treatment of recurrent respiratory papillomatosis of the glottis is often challenging. The surgeon and patient must cooperatively balance decisions regarding airway safety, effects of multiple general anesthesias, employment disturbance, and vocal dysfunction. A pilot study was done in 41 adult cases (23 patients; 78 vocal folds) without complication to evaluate the effectiveness of a 585-nm pulsed dye laser (PDL; 450-μs pulse width; fluence of 38 to 255 J/cm2; 1- to 2-mm spot size) in the treatment of this disorder. Thirty-seven of the 41 cases (90%) were bilateral disease. Twenty-six of the 41 cases (63%; including 20 cases with involvement of the anterior commissure) were treated by bilateral photocoagulation of the lesions' microcirculation without microflap resection of tissue. Clinical observation revealed that irradiated but unresected disease involuted without development of an anterior commissure web. In the initial 13 of the 41 cases (32%), PDL treatment was followed by cold instrument microflap resection. The PDL enhanced the epithelial excision by improving hemostasis and by creating an optimal dissection plane between the basement membrane and the underlying superficial lamina propria. The PDL at 585 nm was less effective in the management of exophytic lesions because of its limited depth of penetration (approximately 2 mm). In this initial trial, the PDL was a relatively safe and efficacious treatment for glottal recurrent respiratory papillomatosis. Since the lesions involute without complete resection of the diseased epithelium, the anterior commissure can be treated to minimize the number of procedures. To study patterns of recurrence will require longer follow-up.

Flashlamp pulsed dye laser (PDL) suppression of keloid proliferation through down-regulation of TGF-?1 expression and extracellular matrix expression

2004 ◽  
Vol 34 (2) ◽  
pp. 104-108 ◽  
Author(s):  
Yur-Ren Kuo ◽  
Seng-Feng Jeng ◽  
Feng-Sheng Wang ◽  
Tien-Hsing Chen ◽  
Hui-Chen Huang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Dye Laser ◽  
Pulsed Dye Laser ◽  
Down Regulation ◽  
Matrix Expression

scholarly journals Treatment of Hemorrhagic Vocal Polyps by Pulsed Dye Laser-Assisted Laryngomicrosurgery

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Hyung Kwon Byeon ◽  
Ji Hyuk Han ◽  
Byeong Il Choi ◽  
Hye Jin Hwang ◽  
Ji-Hoon Kim ◽  
...  
Keyword(s):  
Surgical Technique ◽  
Vocal Fold ◽  
Surgical Techniques ◽  
Dye Laser ◽  
Pulsed Dye Laser ◽  
Vocal Polyp ◽  
Promising Treatment ◽  
Surgical Field ◽  
Professional Voice Users ◽  
Professional Voice

Objective. Conventional surgical techniques of laryngomicrosurgery (LMS) on hemorrhagic vocal polyps are often difficult due to obscuration of the surgical field by inadvertent bleeding from the lesion, and there are often significant amounts of mucosal epithelium loss. Here, we introduce our surgical technique using pulsed dye laser (PDL), which can effectively resect the polyp with vocal fold mucosa preservation.Methods. Patients who were diagnosed with hemorrhagic vocal polyp and who were surgically managed using PDL from March 2013 to October 2014 were retrospectively reviewed. Preoperative and postoperative clinical outcomes and surgical findings were evaluated.Results. A total of 39 patients were treated with PDL-assisted enucleation LMS. The average age was 43.7 years (range 20–73), and there were 20 males and 19 females (17 professional voice users). In all cases, the hemorrhagic polyp was successfully enucleated after application of PDL, thereby preserving the overlying epithelium. Postoperative voice outcomes were favorable with clear preservation of the vocal fold mucosal wave.Conclusion. PDL-assisted enucleation LMS for the treatment of hemorrhagic vocal polyps can be a safe and effective surgical technique. It can be considered a promising treatment option for hemorrhagic vocal polyps.

Sign in / Sign up

Export Citation Format

Share Document