scholarly journals The role of laser fluence in cell viability, proliferation, and membrane integrity of wounded human skin fibroblasts following helium-neon laser irradiation

2006 ◽  
Vol 38 (1) ◽  
pp. 74-83 ◽  
Author(s):  
Denise H. Hawkins ◽  
Heidi Abrahamse
Keyword(s):  
Cell Viability ◽  
Laser Irradiation ◽  
Human Skin ◽  
Laser Fluence ◽  
Membrane Integrity ◽  
Human Skin Fibroblasts ◽  
Neon Laser ◽  
Helium Neon Laser ◽  
Helium Neon

scholarly journals Changes in Cell Viability of Wounded Fibroblasts following Laser Irradiation in Broad-Spectrum or Infrared Light

2007 ◽  
Vol 2007 ◽  
pp. 1-10 ◽  
Author(s):  
Denise Hawkins ◽  
Heidi Abrahamse
Keyword(s):  
Cell Viability ◽  
Broad Spectrum ◽  
Caspase 3 ◽  
Cell Function ◽  
Membrane Integrity ◽  
Infrared Light ◽  
Neon Laser ◽  
Helium Neon Laser ◽  
Helium Neon ◽  
Ir Light

Objective. This study aimed to establish if broad-spectrum or infrared (IR) light in combination with laser therapy can assist phototherapy to improve the cell function of wounded cells. Background. The effect of laser light may be partly or completely reduced by broad-spectrum light. Methods. Wounded human skin fibroblasts were irradiated with 5 J/cm2 using a helium-neon laser, a diode laser, or an Nd:YAG laser in the dark, in the light, or in IR. Changes in cell viability were evaluated by cell morphology, ATP cell viability, LDH membrane integrity, and caspase 3/7 as an early marker of apoptosis. Results. Wounded cells exposed to 5 J/cm2 using 632.8 nm in the dark or 830 nm in the light or 1064 nm in the dark showed an increase in ATP viability, an increase in cytokine expression, and a decrease in LDH cytotoxicity indicating that the metabolic activity of the wounded cells was stimulated. Conclusion. Wounded cells irradiated in IR light showed an undesirable thermal effect that was proportional to the duration of exposure.

Low-Energy Helium-Neon Laser Irradiation Increases the Motility of Cultured Human Keratinocytes

1990 ◽  
Vol 94 (6) ◽  
pp. 822-826 ◽  
Author(s):  
Ann F. Haas ◽  
R. Rivkah Isseroff ◽  
Ronald G. Wheeland ◽  
Pamela A. Rood ◽  
Phillip J. Graves
Keyword(s):  
Laser Irradiation ◽  
Human Keratinocytes ◽  
Low Energy ◽  
Neon Laser ◽  
Helium Neon Laser ◽  
Helium Neon

Helium-neon laser irradiation induces effects on cytokine production at the protein and the mRNA level

1993 ◽  
Vol 2 (2) ◽  
pp. 75-83 ◽  
Author(s):  
Jens Oliver Funk ◽  
Andrea Kruse ◽  
Petra Neustock ◽  
Holger Kirchner
Keyword(s):  
Laser Irradiation ◽  
Cytokine Production ◽  
Mrna Level ◽  
Neon Laser ◽  
Helium Neon Laser ◽  
Helium Neon

Helium–Neon Laser Irradiation: Effect on the Experimental Pain Threshold

2000 ◽  
Vol 15 (3) ◽  
pp. 169-173 ◽  
Author(s):  
O. Schuhfried ◽  
M. Korpan ◽  
V. Fialka-Moser
Keyword(s):  
Laser Irradiation ◽  
Pain Threshold ◽  
Experimental Pain ◽  
Irradiation Effect ◽  
Neon Laser ◽  
Helium Neon Laser ◽  
Helium Neon

scholarly journals CHANGES IN FIBROBLAST PROLIFERATION AND METABOLISM FOLLOWING IN VITRO HELIUM-NEON LASER IRRADIATION

LASER THERAPY ◽  
1991 ◽  
Vol 3 (1) ◽  
pp. 25-33 ◽  
Author(s):  
J. Rigau ◽  
M.A. Trelles ◽  
R.G. Calderhead ◽  
E. Mayayo
Keyword(s):  
Laser Irradiation ◽  
Fibroblast Proliferation ◽  
Neon Laser ◽  
Helium Neon Laser ◽  
Helium Neon

Low-energy helium-neon laser irradiation and the tensile strength of incisional wounds in the rat

1995 ◽  
Vol 3 (4) ◽  
pp. 512-517 ◽  
Author(s):  
Caroline Broadley ◽  
Kenneth N. Broadley ◽  
Gerald Disimone ◽  
Lou Reinisch ◽  
Jeffrey M. Davidson
Keyword(s):  
Tensile Strength ◽  
Laser Irradiation ◽  
Low Energy ◽  
Neon Laser ◽  
Helium Neon Laser ◽  
Helium Neon
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